Prevention of pleural adhesions by bioactive polypeptides - a pilot study.
(2013) In International Journal of Medical Sciences 10(12). p.1720-1726- Abstract
- Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All... (More)
- Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All animals underwent primary pleural surgery, the controls receiving saline in the pleural cavity and the experimental group the PL+PG solution administered by spray. The animals were evaluated on day 7. Macroscopic appearance of adhesions was evaluated by a scoring system. Histology slides of the adhesions and pleural biopsies for evaluation of PAI-1 and TGFb1 were taken on day 7. Results: A significant reduction of adhesions in the PL+PG group (p<0.05) was noted at day 7 both regarding the length and severity of adhesions. There were no significant differences in the concentration of PAI-1 and TGFb1 when comparing the two groups. Conclusions: PL+PG may be used to prevent pleural adhesions. The process of fibrinolysis, and fibrosis was though not affected after PLPG administration. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4143078
- author
- Åkerberg, Daniel LU ; Bauden, Monika LU ; Isaksson, Karolin LU ; Andersson, Roland LU and Tingstedt, Bobby LU
- organization
- publishing date
- 2013
- type
- Contribution to journal
- publication status
- published
- subject
- in
- International Journal of Medical Sciences
- volume
- 10
- issue
- 12
- pages
- 1720 - 1726
- publisher
- Ivyspring International Publisher
- external identifiers
-
- wos:000326099500014
- pmid:24151443
- scopus:84885454796
- pmid:24151443
- ISSN
- 1449-1907
- DOI
- 10.7150/ijms.6651
- language
- English
- LU publication?
- yes
- id
- 5a240c93-0084-49be-9b0f-25d5fc02c679 (old id 4143078)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24151443?dopt=Abstract
- date added to LUP
- 2016-04-01 15:02:17
- date last changed
- 2022-01-28 03:48:35
@article{5a240c93-0084-49be-9b0f-25d5fc02c679, abstract = {{Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All animals underwent primary pleural surgery, the controls receiving saline in the pleural cavity and the experimental group the PL+PG solution administered by spray. The animals were evaluated on day 7. Macroscopic appearance of adhesions was evaluated by a scoring system. Histology slides of the adhesions and pleural biopsies for evaluation of PAI-1 and TGFb1 were taken on day 7. Results: A significant reduction of adhesions in the PL+PG group (p<0.05) was noted at day 7 both regarding the length and severity of adhesions. There were no significant differences in the concentration of PAI-1 and TGFb1 when comparing the two groups. Conclusions: PL+PG may be used to prevent pleural adhesions. The process of fibrinolysis, and fibrosis was though not affected after PLPG administration.}}, author = {{Åkerberg, Daniel and Bauden, Monika and Isaksson, Karolin and Andersson, Roland and Tingstedt, Bobby}}, issn = {{1449-1907}}, language = {{eng}}, number = {{12}}, pages = {{1720--1726}}, publisher = {{Ivyspring International Publisher}}, series = {{International Journal of Medical Sciences}}, title = {{Prevention of pleural adhesions by bioactive polypeptides - a pilot study.}}, url = {{https://lup.lub.lu.se/search/files/4308596/4255000}}, doi = {{10.7150/ijms.6651}}, volume = {{10}}, year = {{2013}}, }