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Prevention of pleural adhesions by bioactive polypeptides - a pilot study.

Åkerberg, Daniel LU ; Bauden, Monika LU ; Isaksson, Karolin LU ; Andersson, Roland LU and Tingstedt, Bobby LU (2013) In International Journal of Medical Sciences 10(12). p.1720-1726
Abstract
Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All... (More)
Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All animals underwent primary pleural surgery, the controls receiving saline in the pleural cavity and the experimental group the PL+PG solution administered by spray. The animals were evaluated on day 7. Macroscopic appearance of adhesions was evaluated by a scoring system. Histology slides of the adhesions and pleural biopsies for evaluation of PAI-1 and TGFb1 were taken on day 7. Results: A significant reduction of adhesions in the PL+PG group (p<0.05) was noted at day 7 both regarding the length and severity of adhesions. There were no significant differences in the concentration of PAI-1 and TGFb1 when comparing the two groups. Conclusions: PL+PG may be used to prevent pleural adhesions. The process of fibrinolysis, and fibrosis was though not affected after PLPG administration. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
International Journal of Medical Sciences
volume
10
issue
12
pages
1720 - 1726
publisher
Ivyspring International Publisher
external identifiers
  • wos:000326099500014
  • pmid:24151443
  • scopus:84885454796
ISSN
1449-1907
DOI
10.7150/ijms.6651
language
English
LU publication?
yes
id
5a240c93-0084-49be-9b0f-25d5fc02c679 (old id 4143078)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24151443?dopt=Abstract
date added to LUP
2013-11-04 22:42:33
date last changed
2019-02-10 04:14:22
@article{5a240c93-0084-49be-9b0f-25d5fc02c679,
  abstract     = {Objective: Postoperative pleural adhesions lead to major problems in repeated thoracic surgery. To date, no antiadhesive product has been proven clinically effective. Previous studies of differently charged polypeptides, poly-L-lysine (PL) and poly-L-glutamate (PG) have shown promising results reducing postoperative abdominal adhesions in experimental settings. This pilot study examined the possible pleural adhesion prevention by using the PL+PG concept after pleural surgery and its possible effect on key parameters; plasmin activator inhibitor-1 (PAI-1) and tissue growth factor beta 1 (TGFb) in the fibrinolytic process. Methods: A total of 22 male rats were used in the study, one control group (n=10) and one experimental group (n=12). All animals underwent primary pleural surgery, the controls receiving saline in the pleural cavity and the experimental group the PL+PG solution administered by spray. The animals were evaluated on day 7. Macroscopic appearance of adhesions was evaluated by a scoring system. Histology slides of the adhesions and pleural biopsies for evaluation of PAI-1 and TGFb1 were taken on day 7. Results: A significant reduction of adhesions in the PL+PG group (p&lt;0.05) was noted at day 7 both regarding the length and severity of adhesions. There were no significant differences in the concentration of PAI-1 and TGFb1 when comparing the two groups. Conclusions: PL+PG may be used to prevent pleural adhesions. The process of fibrinolysis, and fibrosis was though not affected after PLPG administration.},
  author       = {Åkerberg, Daniel and Bauden, Monika and Isaksson, Karolin and Andersson, Roland and Tingstedt, Bobby},
  issn         = {1449-1907},
  language     = {eng},
  number       = {12},
  pages        = {1720--1726},
  publisher    = {Ivyspring International Publisher},
  series       = {International Journal of Medical Sciences},
  title        = {Prevention of pleural adhesions by bioactive polypeptides - a pilot study.},
  url          = {http://dx.doi.org/10.7150/ijms.6651},
  volume       = {10},
  year         = {2013},
}