Nuclear localization of {gamma}-tubulin affects E2F transcriptional activity and S-phase progression.
(2011) In FASEB Journal 25(11). p.3815-3827- Abstract
- We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus... (More)
- We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus encodes a DNA-binding domain that interacts with E2F-regulated promoters, resulting in γ-tubulin-mediated transient activation of E2Fs. Thus, we report a novel mechanism regulating the activity of E2Fs, which can help explain how these proteins affect cell cycle progression in mammalian cells.-Höög, G., Zarrizi, R., von Stedingk, K., Jonsson, K., Alvarado-Kristensson, M. Nuclear localization of γ-tubulin affects E2F transcriptional activity and S-phase progression. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/2151810
- author
- Eklund, Greta LU ; Zarrizi, Reihaneh LU ; von Stedingk, Kristoffer LU ; Jönsson, Kristina and Alvarado-Kristensson, Maria LU
- organization
- publishing date
- 2011
- type
- Contribution to journal
- publication status
- published
- subject
- in
- FASEB Journal
- volume
- 25
- issue
- 11
- pages
- 3815 - 3827
- publisher
- Wiley
- external identifiers
-
- wos:000296723100007
- pmid:21788450
- scopus:80355148509
- pmid:21788450
- ISSN
- 1530-6860
- DOI
- 10.1096/fj.11-187484
- language
- English
- LU publication?
- yes
- additional info
- Department affilation moved from v1000583 (Molecular Tumour Biology) to v1000562 (Department of Translational Medicine) on 2016-01-18 14:41:46.
- id
- 5a9e4c46-1b94-4181-be2f-eb0cef6ac397 (old id 2151810)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21788450?dopt=Abstract
- date added to LUP
- 2016-04-01 09:54:33
- date last changed
- 2024-02-20 23:33:01
@article{5a9e4c46-1b94-4181-be2f-eb0cef6ac397, abstract = {{We show that the centrosome- and microtubule-regulating protein γ-tubulin interacts with E2 promoter binding factors (E2Fs) to modulate E2F transcriptional activity and thereby control cell cycle progression. γ-Tubulin contains a C-terminal signal that results in its translocation to the nucleus during late G(1) to early S phase. γ-Tubulin mutants showed that the C terminus interacts with the transcription factor E2F1 and that the E2F1-γ-tubulin complex is formed during the G(1)/S transition, when E2F1 is transcriptionally active. Furthermore, E2F transcriptional activity is altered by reduced expression of γ-tubulin or by complex formation between γ-tubulin and E2F1, E2F2, or E2F3, but not E2F6. In addition, the γ-tubulin C terminus encodes a DNA-binding domain that interacts with E2F-regulated promoters, resulting in γ-tubulin-mediated transient activation of E2Fs. Thus, we report a novel mechanism regulating the activity of E2Fs, which can help explain how these proteins affect cell cycle progression in mammalian cells.-Höög, G., Zarrizi, R., von Stedingk, K., Jonsson, K., Alvarado-Kristensson, M. Nuclear localization of γ-tubulin affects E2F transcriptional activity and S-phase progression.}}, author = {{Eklund, Greta and Zarrizi, Reihaneh and von Stedingk, Kristoffer and Jönsson, Kristina and Alvarado-Kristensson, Maria}}, issn = {{1530-6860}}, language = {{eng}}, number = {{11}}, pages = {{3815--3827}}, publisher = {{Wiley}}, series = {{FASEB Journal}}, title = {{Nuclear localization of {gamma}-tubulin affects E2F transcriptional activity and S-phase progression.}}, url = {{http://dx.doi.org/10.1096/fj.11-187484}}, doi = {{10.1096/fj.11-187484}}, volume = {{25}}, year = {{2011}}, }