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Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts

Bornman, Janet LU ; Bornman, C H and Björn, Lars Olof LU orcid (1982) In Zeitschrift für Pflanzenphysiologie 105(4). p.297-306
Abstract
Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B... (More)
Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged. (Less)
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publication status
published
subject
in
Zeitschrift für Pflanzenphysiologie
volume
105
issue
4
pages
297 - 306
publisher
Gustav Fischer Verlag
ISSN
0044-328X
language
English
LU publication?
yes
id
5bf3b6c7-efb6-4f12-bc66-2b5480bf4563 (old id 134623)
date added to LUP
2016-04-01 15:54:29
date last changed
2018-11-21 20:37:17
@article{5bf3b6c7-efb6-4f12-bc66-2b5480bf4563,
  abstract     = {{Estimates of viability as measured by vital staining with fluorescein diacetate (FDA) were carried out on freshly isolated and partially aged (16-h-old) B. vulgaris and H. vulgare mesophyll protoplasts following irradiation with UV-B. Damage to the photosynthetic system by UV-B was determined by delayed light emission (DLE). In the case of freshly isolated protoplasts Beta was .apprx. 30% more susceptible than Hordeum following 3 h irradiation, with viability decreasing from 90% to 40%. After storage of protoplasts on ice for 16 h UV-B radiation markedly depressed viability in both species, but in the case of Hordeum there was a substantial initial loss of nearly 70% in viability over the 1st hour of irradiation. The first 10 min of UV-B radiation decreased the intensity of DLE by 40% without appreciably affecting the decay rate. Longer treatment times did not give a proportional effect so that even after 60 min of UV-B the inhibition did not exceed 60%. This suggested that although the enzyme system responsible for FDA hydrolysis may be partially inactivated (viability was 75-80% as compared with 90% in the control), the UV-B did not penetrate the innermost parts of the chloroplasts, but left some thylakoids undamaged.}},
  author       = {{Bornman, Janet and Bornman, C H and Björn, Lars Olof}},
  issn         = {{0044-328X}},
  language     = {{eng}},
  number       = {{4}},
  pages        = {{297--306}},
  publisher    = {{Gustav Fischer Verlag}},
  series       = {{Zeitschrift für Pflanzenphysiologie}},
  title        = {{Effects of ultraviolet-radiation on viability of isolated beta-vulgaris and hordeum-vulgare protoplasts}},
  volume       = {{105}},
  year         = {{1982}},
}