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Bioartificial nerve graft for bridging extended nerve defects in rat sciatic nerve based on resorbable guiding filaments

Arai, Takeru ; Lundborg, Göran LU and Dahlin, Lars B. LU (2000) In Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery 34(2). p.101-108
Abstract

A long defect (15 mm) in rat sciatic nerve was repaired with a bioartificial nerve graft composed of a silicone tube and seven synthetic filaments of five types (polyamide, catgut, polydioxanone, and two types of polyglactin, normal and quickly-absorbed) inserted longitudinally into the tube. In all cases in which filaments were used a regenerating bridge was obtained in the tube after three months in contrast to empty silicone tubes, in which no structure was observed. There was a 6% ~ 46% recovery of isometric muscle contractility of the anterior tibial and gastrocnemius muscles with positive pinch reflex test in most cases. Myelinated axons were seen in the regenerating tissue between the filaments but not directly in contact with... (More)

A long defect (15 mm) in rat sciatic nerve was repaired with a bioartificial nerve graft composed of a silicone tube and seven synthetic filaments of five types (polyamide, catgut, polydioxanone, and two types of polyglactin, normal and quickly-absorbed) inserted longitudinally into the tube. In all cases in which filaments were used a regenerating bridge was obtained in the tube after three months in contrast to empty silicone tubes, in which no structure was observed. There was a 6% ~ 46% recovery of isometric muscle contractility of the anterior tibial and gastrocnemius muscles with positive pinch reflex test in most cases. Myelinated axons were seen in the regenerating tissue between the filaments but not directly in contact with them, and there were varying numbers of macrophages close to the filaments. Silicone tubes with filaments, regardless of type of filament, induced nerve tissue to regenerate and resulted in functional recovery through a 15 mm nerve gap not achieved with empty tubes. Nerve promoting factors may be applied to the filaments and the model is a valuable tool for further development of artificial nerve grafts.

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Axonal growth, Biomaterial, Nerve graft, Nerve regeneration, Resorbable filaments, Silicone chamber
in
Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery
volume
34
issue
2
pages
8 pages
publisher
Taylor & Francis
external identifiers
  • pmid:10900623
  • scopus:0033915598
ISSN
0284-4311
DOI
10.1080/02844310050159936
language
English
LU publication?
yes
id
5cea6a49-b7aa-464e-8ef9-cb9803b8b13d
date added to LUP
2019-06-17 13:56:09
date last changed
2021-05-18 04:19:36
@article{5cea6a49-b7aa-464e-8ef9-cb9803b8b13d,
  abstract     = {<p>A long defect (15 mm) in rat sciatic nerve was repaired with a bioartificial nerve graft composed of a silicone tube and seven synthetic filaments of five types (polyamide, catgut, polydioxanone, and two types of polyglactin, normal and quickly-absorbed) inserted longitudinally into the tube. In all cases in which filaments were used a regenerating bridge was obtained in the tube after three months in contrast to empty silicone tubes, in which no structure was observed. There was a 6% ~ 46% recovery of isometric muscle contractility of the anterior tibial and gastrocnemius muscles with positive pinch reflex test in most cases. Myelinated axons were seen in the regenerating tissue between the filaments but not directly in contact with them, and there were varying numbers of macrophages close to the filaments. Silicone tubes with filaments, regardless of type of filament, induced nerve tissue to regenerate and resulted in functional recovery through a 15 mm nerve gap not achieved with empty tubes. Nerve promoting factors may be applied to the filaments and the model is a valuable tool for further development of artificial nerve grafts.</p>},
  author       = {Arai, Takeru and Lundborg, Göran and Dahlin, Lars B.},
  issn         = {0284-4311},
  language     = {eng},
  month        = {08},
  number       = {2},
  pages        = {101--108},
  publisher    = {Taylor & Francis},
  series       = {Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery},
  title        = {Bioartificial nerve graft for bridging extended nerve defects in rat sciatic nerve based on resorbable guiding filaments},
  url          = {http://dx.doi.org/10.1080/02844310050159936},
  doi          = {10.1080/02844310050159936},
  volume       = {34},
  year         = {2000},
}