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Transcription of human endogenous retroviral sequences related to mouse mammary tumor virus in human breast and placenta : similar pattern in most malignant and nonmalignant breast tissues

Yin, H; Medstrand, P LU ; Andersson, M L LU ; Borg, Åke LU ; Olsson, Håkan LU and Blomberg, J (1997) In AIDS Research and Human Retroviruses 13(6). p.507-516
Abstract

The human genome contains a large variety of sequences related to the mouse mammary tumor virus (MMTV). We have investigated the range of expression of human endogenous retroviral sequences (HERVs) related to MMTV (human MMTV-like; HML) as RNA in 60 breast cancers, 8 nonmalignant breast tissues, and 9 placentas. This was monitored using HML group-specific oligonucleotide probes in hybridizations toward PCR amplificates of HML pol sequences and internal control. The degree of expression of five HML groups varied between individuals and between tissues. On average, all HML groups were less expressed in breast tissues than in placenta. The hybridization signals of some HML RNAs were strongly correlated, indicating a nonstochastic mechanism... (More)

The human genome contains a large variety of sequences related to the mouse mammary tumor virus (MMTV). We have investigated the range of expression of human endogenous retroviral sequences (HERVs) related to MMTV (human MMTV-like; HML) as RNA in 60 breast cancers, 8 nonmalignant breast tissues, and 9 placentas. This was monitored using HML group-specific oligonucleotide probes in hybridizations toward PCR amplificates of HML pol sequences and internal control. The degree of expression of five HML groups varied between individuals and between tissues. On average, all HML groups were less expressed in breast tissues than in placenta. The hybridization signals of some HML RNAs were strongly correlated, indicating a nonstochastic mechanism and a concerted regulation of their expression. The PCR product from one breast cancer (BC 6), which gave an exceptionally high expression with probe hml-6, with a 20 times stronger signal than the rest of the cancers, was cloned and sequenced. The HML-6 transcript sequences were homogeneous in BC 6. The most predominant clone derived from the cancer was used as a probe in Southern hybridizations. The same restriction fragments were detected in human breast tissues, PBMCs (peripheral blood mononuclear cells), and breast cancer cell lines, except for one of the breast cancers and one of the nonmalignant breast tissues, which gave different banding patterns. A comparison of HML expression in normal and malignant breast tissue from the same individual would have been more precise than our comparison of samples from different persons. Bearing this limitation in mind, with a single exception, human MMTV-like sequences were not more actively expressed in malignant than in nonmalignant breast tissues. Nevertheless, an interesting diversity in their expression, especially between individuals, was found.

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keywords
Adult, Aged, Aged, 80 and over, Amino Acid Sequence, Animals, Base Sequence, Breast, Breast Neoplasms, Cell Line, DNA, Complementary, DNA, Viral, Female, Gene Expression, Genome, Viral, Humans, Mammary Tumor Virus, Mouse, Mice, Middle Aged, Molecular Sequence Data, Placenta, Retroviridae, Sequence Homology, Nucleic Acid, Transcription, Genetic
in
AIDS Research and Human Retroviruses
volume
13
issue
6
pages
507 - 516
publisher
Mary Ann Liebert, Inc.
external identifiers
  • Scopus:0030890916
ISSN
0889-2229
DOI
10.1089/aid.1997.13.507
language
English
LU publication?
yes
id
5cf83501-bc4c-4be6-8057-8fb097451f4f
date added to LUP
2016-09-18 12:47:03
date last changed
2017-01-01 08:34:09
@article{5cf83501-bc4c-4be6-8057-8fb097451f4f,
  abstract     = {<p>The human genome contains a large variety of sequences related to the mouse mammary tumor virus (MMTV). We have investigated the range of expression of human endogenous retroviral sequences (HERVs) related to MMTV (human MMTV-like; HML) as RNA in 60 breast cancers, 8 nonmalignant breast tissues, and 9 placentas. This was monitored using HML group-specific oligonucleotide probes in hybridizations toward PCR amplificates of HML pol sequences and internal control. The degree of expression of five HML groups varied between individuals and between tissues. On average, all HML groups were less expressed in breast tissues than in placenta. The hybridization signals of some HML RNAs were strongly correlated, indicating a nonstochastic mechanism and a concerted regulation of their expression. The PCR product from one breast cancer (BC 6), which gave an exceptionally high expression with probe hml-6, with a 20 times stronger signal than the rest of the cancers, was cloned and sequenced. The HML-6 transcript sequences were homogeneous in BC 6. The most predominant clone derived from the cancer was used as a probe in Southern hybridizations. The same restriction fragments were detected in human breast tissues, PBMCs (peripheral blood mononuclear cells), and breast cancer cell lines, except for one of the breast cancers and one of the nonmalignant breast tissues, which gave different banding patterns. A comparison of HML expression in normal and malignant breast tissue from the same individual would have been more precise than our comparison of samples from different persons. Bearing this limitation in mind, with a single exception, human MMTV-like sequences were not more actively expressed in malignant than in nonmalignant breast tissues. Nevertheless, an interesting diversity in their expression, especially between individuals, was found.</p>},
  author       = {Yin, H and Medstrand, P and Andersson, M L and Borg, Åke and Olsson, Håkan and Blomberg, J},
  issn         = {0889-2229},
  keyword      = {Adult,Aged,Aged, 80 and over,Amino Acid Sequence,Animals,Base Sequence,Breast,Breast Neoplasms,Cell Line,DNA, Complementary,DNA, Viral,Female,Gene Expression,Genome, Viral,Humans,Mammary Tumor Virus, Mouse,Mice,Middle Aged,Molecular Sequence Data,Placenta,Retroviridae,Sequence Homology, Nucleic Acid,Transcription, Genetic},
  language     = {eng},
  month        = {04},
  number       = {6},
  pages        = {507--516},
  publisher    = {Mary Ann Liebert, Inc.},
  series       = {AIDS Research and Human Retroviruses},
  title        = {Transcription of human endogenous retroviral sequences related to mouse mammary tumor virus in human breast and placenta : similar pattern in most malignant and nonmalignant breast tissues},
  url          = {http://dx.doi.org/10.1089/aid.1997.13.507},
  volume       = {13},
  year         = {1997},
}