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Coexpression of neurotrophins and their receptors in neurons of the central nervous system

Kokaia, Z LU orcid ; Bengzon, J LU ; Metsis, M ; Kokaia, M LU ; Persson, H and Lindvall, O LU (1993) In Proceedings of the National Academy of Sciences of the United States of America 90(14). p.5-6711
Abstract

Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only... (More)

Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.

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author
; ; ; ; and
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Animals, Brain/cytology, Brain Chemistry, Brain-Derived Neurotrophic Factor, Cerebral Cortex/chemistry, Hippocampus/chemistry, In Situ Hybridization, Male, Membrane Proteins/isolation & purification, Nerve Growth Factors/isolation & purification, Nerve Tissue Proteins/isolation & purification, Neurons/chemistry, RNA, Messenger/isolation & purification, Rats, Rats, Sprague-Dawley, Receptor, Ciliary Neurotrophic Factor, Seizures
in
Proceedings of the National Academy of Sciences of the United States of America
volume
90
issue
14
pages
5 pages
publisher
National Academy of Sciences
external identifiers
  • pmid:8341689
  • scopus:0027292245
ISSN
0027-8424
DOI
10.1073/pnas.90.14.6711
language
English
LU publication?
no
id
5d6f622a-1d28-43a3-b395-fa4fa4213e06
date added to LUP
2019-06-25 10:41:59
date last changed
2024-01-01 13:06:48
@article{5d6f622a-1d28-43a3-b395-fa4fa4213e06,
  abstract     = {{<p>Nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are neuronal survival molecules which utilize the Trk family of tyrosine kinase receptors. Using double-label in situ hybridization, we demonstrate that mRNAs for BDNF and its high-affinity receptor TrkB are coexpressed in hippocampal and cortical neurons. Also, a large number of neurons in these areas coexpress NGF and BDNF mRNAs. Epileptic seizures lead to increased levels of both BDNF/TrkB and NGF/BDNF mRNAs in double-labeled cells. Our results show that individual neurons of the central nervous system can coexpress neurotrophins and their receptors and produce two neurotrophic factors. These factors could support neuronal survival after brain insults, not only via retrograde transport but also through autocrine mechanisms.</p>}},
  author       = {{Kokaia, Z and Bengzon, J and Metsis, M and Kokaia, M and Persson, H and Lindvall, O}},
  issn         = {{0027-8424}},
  keywords     = {{Animals; Brain/cytology; Brain Chemistry; Brain-Derived Neurotrophic Factor; Cerebral Cortex/chemistry; Hippocampus/chemistry; In Situ Hybridization; Male; Membrane Proteins/isolation & purification; Nerve Growth Factors/isolation & purification; Nerve Tissue Proteins/isolation & purification; Neurons/chemistry; RNA, Messenger/isolation & purification; Rats; Rats, Sprague-Dawley; Receptor, Ciliary Neurotrophic Factor; Seizures}},
  language     = {{eng}},
  month        = {{07}},
  number       = {{14}},
  pages        = {{5--6711}},
  publisher    = {{National Academy of Sciences}},
  series       = {{Proceedings of the National Academy of Sciences of the United States of America}},
  title        = {{Coexpression of neurotrophins and their receptors in neurons of the central nervous system}},
  url          = {{http://dx.doi.org/10.1073/pnas.90.14.6711}},
  doi          = {{10.1073/pnas.90.14.6711}},
  volume       = {{90}},
  year         = {{1993}},
}