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Altered dermatan sulfate proteoglycan synthesis in fibroblast cultures established from skin of patients with systemic sclerosis

Westergren-Thorsson, G LU ; Cöster, Lars; Akesson, A LU and Wollheim, F A LU (1996) In Journal of Rheumatology 23(8). p.406-1398
Abstract

OBJECTIVE: To study whether changes in the properties of skin from patients with systemic sclerosis (SSc) are the result of altered metabolism of dermatan sulfate proteoglycans.

METHODS: Fibroblast cultures were established from skin of healthy controls, and from affected and unaffected skin of patients with SSc. Synthesized proteoglycans were labeled with 3H glucosamine and 35S sulfate. The amount of mRNA of the different dermatan sulfate proteoglycans was determined by hybridization with the corresponding cDNA probes.

RESULTS: A 2-fold increase in secretion of total proteoglycans was found in cell cultures from affected and normal appearing skin from patients with SSc. The production of 2 different dermatan sulfate... (More)

OBJECTIVE: To study whether changes in the properties of skin from patients with systemic sclerosis (SSc) are the result of altered metabolism of dermatan sulfate proteoglycans.

METHODS: Fibroblast cultures were established from skin of healthy controls, and from affected and unaffected skin of patients with SSc. Synthesized proteoglycans were labeled with 3H glucosamine and 35S sulfate. The amount of mRNA of the different dermatan sulfate proteoglycans was determined by hybridization with the corresponding cDNA probes.

RESULTS: A 2-fold increase in secretion of total proteoglycans was found in cell cultures from affected and normal appearing skin from patients with SSc. The production of 2 different dermatan sulfate proteoglycans was increased. Aggrecan/versican increased 4-fold and decorin 2-fold in cultures of affected skin from patients with SSc. The mRNA for decorin increased 3-fold, while the mRNA level for versican increased only slightly. Similar but less marked changes were noted in cultures from normal appearing skin. In contrast, the biglycan mRNA level decreased and the product could only be found in very small amounts in SSc cultures.

CONCLUSION: This marked alteration of dermatan sulfate proteoglycan metabolism distinguishes not only affected skin but also normal appearing SSc skin from that of controls. The altered proteoglycan production may affect organization of matrix fibers and thereby the fibrotic process observed in patients with SSc.

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keywords
Adult, Aged, Aged, 80 and over, Biopsy, Cells, Cultured, Chondroitin Sulfate Proteoglycans, Chromatography, Gel, Connective Tissue, Dermatan Sulfate, Female, Fibroblasts, Humans, Male, Middle Aged, Proteoglycans, RNA, Messenger, Scleroderma, Systemic, Journal Article, Research Support, Non-U.S. Gov't
in
Journal of Rheumatology
volume
23
issue
8
pages
406 - 1398
publisher
J Rheumatol Publ Co
external identifiers
  • scopus:0029758544
ISSN
0315-162X
language
English
LU publication?
yes
id
5da41441-3ebb-4188-84cd-36bb5218c19e
date added to LUP
2017-06-27 14:18:53
date last changed
2017-07-02 05:03:55
@article{5da41441-3ebb-4188-84cd-36bb5218c19e,
  abstract     = {<p>OBJECTIVE: To study whether changes in the properties of skin from patients with systemic sclerosis (SSc) are the result of altered metabolism of dermatan sulfate proteoglycans.</p><p>METHODS: Fibroblast cultures were established from skin of healthy controls, and from affected and unaffected skin of patients with SSc. Synthesized proteoglycans were labeled with 3H glucosamine and 35S sulfate. The amount of mRNA of the different dermatan sulfate proteoglycans was determined by hybridization with the corresponding cDNA probes.</p><p>RESULTS: A 2-fold increase in secretion of total proteoglycans was found in cell cultures from affected and normal appearing skin from patients with SSc. The production of 2 different dermatan sulfate proteoglycans was increased. Aggrecan/versican increased 4-fold and decorin 2-fold in cultures of affected skin from patients with SSc. The mRNA for decorin increased 3-fold, while the mRNA level for versican increased only slightly. Similar but less marked changes were noted in cultures from normal appearing skin. In contrast, the biglycan mRNA level decreased and the product could only be found in very small amounts in SSc cultures.</p><p>CONCLUSION: This marked alteration of dermatan sulfate proteoglycan metabolism distinguishes not only affected skin but also normal appearing SSc skin from that of controls. The altered proteoglycan production may affect organization of matrix fibers and thereby the fibrotic process observed in patients with SSc.</p>},
  author       = {Westergren-Thorsson, G and Cöster, Lars and Akesson, A and Wollheim, F A},
  issn         = {0315-162X},
  keyword      = {Adult,Aged,Aged, 80 and over,Biopsy,Cells, Cultured,Chondroitin Sulfate Proteoglycans,Chromatography, Gel,Connective Tissue,Dermatan Sulfate,Female,Fibroblasts,Humans,Male,Middle Aged,Proteoglycans,RNA, Messenger,Scleroderma, Systemic,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {8},
  pages        = {406--1398},
  publisher    = {J Rheumatol Publ Co},
  series       = {Journal of Rheumatology},
  title        = {Altered dermatan sulfate proteoglycan synthesis in fibroblast cultures established from skin of patients with systemic sclerosis},
  volume       = {23},
  year         = {1996},
}