High density supercritical carbon dioxide for the extraction of pesticide residues in onion with multivariate response surface methodology
Tolcha, Teshome ; Gemechu, Tura ; Al‐Hamimi, Said LU ; Megersa, Negussie LU and Turner, Charlotta LU
(2020)
In Molecules
25(4).
- Abstract
The excessive use of pesticides is a serious health problem due to their toxicity and bioaccumulation through the food chain. Due to the complexity of foods, the analysis of pesticides is challenging often giving large matrix effects and co‐extracted compounds. To overcome this problem, a selective and “green” supercritical fluid extraction method was developed, using neat carbon dioxide as a solvent at pressures of up to 800 bars. A Box–Behnken response surface experimental design was used, with the independent variables of density (0.70–1.0 g mL–1), temperature (40–70 °C), and volume (10–40 mL) of solvent, and the dependent variable of extracted amount of pesticides. The optimum extraction condition was found at the use of... (More)
The excessive use of pesticides is a serious health problem due to their toxicity and bioaccumulation through the food chain. Due to the complexity of foods, the analysis of pesticides is challenging often giving large matrix effects and co‐extracted compounds. To overcome this problem, a selective and “green” supercritical fluid extraction method was developed, using neat carbon dioxide as a solvent at pressures of up to 800 bars. A Box–Behnken response surface experimental design was used, with the independent variables of density (0.70–1.0 g mL–1), temperature (40–70 °C), and volume (10–40 mL) of solvent, and the dependent variable of extracted amount of pesticides. The optimum extraction condition was found at the use of 29 mL of supercritical CO2 at 0.90 g mL–1 and 53°C (corresponding to 372 bars of pressure). It was observed that increasing the density of CO2 significantly increased the extraction recovery of endrin and 2,4′-dichlorodiphenyldichloroethane. Matrix‐matched calibration curves showed satisfactory linearity (R2 ≥ 0.994), and LODs ranged from 0.2 to 2.0 ng g–1. Precision was lower than 11% and recoveries between 80%–103%. Thus, the developed method could efficiently be used for trace analysis of pesticides in complex food matrices without the use of organic solvents.
(Less)
- author
- Tolcha, Teshome
; Gemechu, Tura
; Al‐Hamimi, Said
LU
; Megersa, Negussie
LU
and Turner, Charlotta
LU
- organization
- publishing date
- 2020-02-24
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Density, Onion, Organochlorine, Pesticide residues, Supercritical fluid extraction, S‐triazine, Ultrahigh pressure
- in
- Molecules
- volume
- 25
- issue
- 4
- article number
- 1012
- publisher
- MDPI AG
- external identifiers
-
- scopus:85079863323
- pmid:32102410
- ISSN
- 1420-3049
- DOI
- 10.3390/molecules25041012
- language
- English
- LU publication?
- yes
- id
- 5ea67694-e65d-43d3-97b3-575ed4e5c2cc
- date added to LUP
- 2020-03-11 11:15:19
- date last changed
- 2024-06-26 12:12:18
@article{5ea67694-e65d-43d3-97b3-575ed4e5c2cc,
abstract = {{<p>The excessive use of pesticides is a serious health problem due to their toxicity and bioaccumulation through the food chain. Due to the complexity of foods, the analysis of pesticides is challenging often giving large matrix effects and co‐extracted compounds. To overcome this problem, a selective and “green” supercritical fluid extraction method was developed, using neat carbon dioxide as a solvent at pressures of up to 800 bars. A Box–Behnken response surface experimental design was used, with the independent variables of density (0.70–1.0 g mL–<sup>1</sup>), temperature (40–70 °C), and volume (10–40 mL) of solvent, and the dependent variable of extracted amount of pesticides. The optimum extraction condition was found at the use of 29 mL of supercritical CO2 at 0.90 g mL–1 and 53°C (corresponding to 372 bars of pressure). It was observed that increasing the density of CO2 significantly increased the extraction recovery of endrin and 2,4′-dichlorodiphenyldichloroethane. Matrix‐matched calibration curves showed satisfactory linearity (R<sup>2</sup> ≥ 0.994), and LODs ranged from 0.2 to 2.0 ng g<sup>–1</sup>. Precision was lower than 11% and recoveries between 80%–103%. Thus, the developed method could efficiently be used for trace analysis of pesticides in complex food matrices without the use of organic solvents.</p>}},
author = {{Tolcha, Teshome and Gemechu, Tura and Al‐Hamimi, Said and Megersa, Negussie and Turner, Charlotta}},
issn = {{1420-3049}},
keywords = {{Density; Onion; Organochlorine; Pesticide residues; Supercritical fluid extraction; S‐triazine; Ultrahigh pressure}},
language = {{eng}},
month = {{02}},
number = {{4}},
publisher = {{MDPI AG}},
series = {{Molecules}},
title = {{High density supercritical carbon dioxide for the extraction of pesticide residues in onion with multivariate response surface methodology}},
url = {{http://dx.doi.org/10.3390/molecules25041012}},
doi = {{10.3390/molecules25041012}},
volume = {{25}},
year = {{2020}},
}