Critical Role of P-Selectin and Lymphocyte Function Antigen-1 in Radiation-Induced Leukocyte-Endothelial Cell Interactions in the Colon.
(2007) In Diseases of the Colon & Rectum 50(12). p.2194-2202- Abstract
- Purpose Radiation therapy is frequently used in treating different types of tumors, although associated with serious side effects, such as fibrosis and complicated diarrhea. This study was designed to define the adhesive mechanisms behind radiotherapy-induced leukocyte recruitment in the colon.
Methods All mice, except control animals, were radiated with a single dose of 20 Gy. Mice were pretreated with an isotype-matched control antibody or a monoclonal antibody directed against P-selectin. In separate experiments, lymphocyte function antigen-1–deficient animals were used. Leukocyte rolling and firm adhesion were determined by use of inverted intravital fluorescence microscopy 16 hours after radiation.
Results It was... (More) - Purpose Radiation therapy is frequently used in treating different types of tumors, although associated with serious side effects, such as fibrosis and complicated diarrhea. This study was designed to define the adhesive mechanisms behind radiotherapy-induced leukocyte recruitment in the colon.
Methods All mice, except control animals, were radiated with a single dose of 20 Gy. Mice were pretreated with an isotype-matched control antibody or a monoclonal antibody directed against P-selectin. In separate experiments, lymphocyte function antigen-1–deficient animals were used. Leukocyte rolling and firm adhesion were determined by use of inverted intravital fluorescence microscopy 16 hours after radiation.
Results It was found that immunoneutralization of P-selectin reduced leukocyte rolling by 83 percent and adhesion by 87 percent in radiated mice. Moreover, radiation-induced leukocyte adhesion in LFA-1-deficient mice was decreased by 94 percent compared with wild-type animals.
Conclusions This study demonstrates that leukocyte rolling is mediated by P-selectin and that firm leukocyte adhesion is supported by lymphocyte function antigen-1 in radiation-induced enteritis. Moreover, P-selectin-dependent leukocyte rolling is a precondition for subsequent leukocyte adhesion in radiation-induced intestinal injury. Thus, targeting P-selectin and/or lymphocyte function antigen-1 may protect against pathologic inflammation in the colon induced by radiotherapy. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/607851
- author
- Röme, Andrada LU ; Thornberg, Charlotte LU ; Mattsson, Sören LU ; Wang, Yusheng LU ; Jeppsson, Bengt LU and Thorlacius, Henrik LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Diseases of the Colon & Rectum
- volume
- 50
- issue
- 12
- pages
- 2194 - 2202
- publisher
- Springer
- external identifiers
-
- wos:000251641700027
- scopus:37249027788
- pmid:17851717
- ISSN
- 0012-3706
- DOI
- 10.1007/s10350-007-9065-7
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Emergency medicine/Medicine/Surgery (013240200), Medical Radiation Physics, Malmö (013243210), Surgery Research Unit (013242220)
- id
- 48da384a-a98c-4c81-88e4-d1bc156c9e46 (old id 607851)
- alternative location
- http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17851717&dopt=Abstract
- date added to LUP
- 2016-04-01 12:15:06
- date last changed
- 2022-01-27 01:00:56
@article{48da384a-a98c-4c81-88e4-d1bc156c9e46,
abstract = {{Purpose Radiation therapy is frequently used in treating different types of tumors, although associated with serious side effects, such as fibrosis and complicated diarrhea. This study was designed to define the adhesive mechanisms behind radiotherapy-induced leukocyte recruitment in the colon.<br/><br>
Methods All mice, except control animals, were radiated with a single dose of 20 Gy. Mice were pretreated with an isotype-matched control antibody or a monoclonal antibody directed against P-selectin. In separate experiments, lymphocyte function antigen-1–deficient animals were used. Leukocyte rolling and firm adhesion were determined by use of inverted intravital fluorescence microscopy 16 hours after radiation.<br/><br>
Results It was found that immunoneutralization of P-selectin reduced leukocyte rolling by 83 percent and adhesion by 87 percent in radiated mice. Moreover, radiation-induced leukocyte adhesion in LFA-1-deficient mice was decreased by 94 percent compared with wild-type animals.<br/><br>
Conclusions This study demonstrates that leukocyte rolling is mediated by P-selectin and that firm leukocyte adhesion is supported by lymphocyte function antigen-1 in radiation-induced enteritis. Moreover, P-selectin-dependent leukocyte rolling is a precondition for subsequent leukocyte adhesion in radiation-induced intestinal injury. Thus, targeting P-selectin and/or lymphocyte function antigen-1 may protect against pathologic inflammation in the colon induced by radiotherapy.}},
author = {{Röme, Andrada and Thornberg, Charlotte and Mattsson, Sören and Wang, Yusheng and Jeppsson, Bengt and Thorlacius, Henrik}},
issn = {{0012-3706}},
language = {{eng}},
number = {{12}},
pages = {{2194--2202}},
publisher = {{Springer}},
series = {{Diseases of the Colon & Rectum}},
title = {{Critical Role of P-Selectin and Lymphocyte Function Antigen-1 in Radiation-Induced Leukocyte-Endothelial Cell Interactions in the Colon.}},
url = {{http://dx.doi.org/10.1007/s10350-007-9065-7}},
doi = {{10.1007/s10350-007-9065-7}},
volume = {{50}},
year = {{2007}},
}