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Estimation of depth of fluorescing lesions in tissue from changes in fluorescence spectra

Swartling, Johannes LU ; Bengtsson, D; Terike, K; Svensson, Jenny LU and Andersson-Engels, Stefan LU (2005) Optical tomography and spectroscopy of tissue VI, 2005 In Proceedings of the SPIE - The International Society for Optical Engineering 5693(1). p.225-231
Abstract
We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the... (More)
We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the lesion. We have performed Monte Carlo simulations and measurements on an Intralipid phantom in the wavelength range 850 - 1000 nm. By taking the ratio between the signals at the wavelengths 875 and 930 nm the depth of a fluorescing layer could be determined with 0.8 mm accuracy down at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types with various composition. The results indicate that depth estimation of a lesion is possible with no assumptions made about the optical properties for a wide range of tissues (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
intralipid phantom, spectroscopy, photon migration, turbid media, 850 to 1000 nm, 10 mm, Monte Carlo simulation, fluorophore, tissue optical property, light absorption, light scattering, excitation light irradiation, fluorescence signal measurement, biological tissue, fluorescent lesion, depth estimation, fluorescence spectra
in
Proceedings of the SPIE - The International Society for Optical Engineering
volume
5693
issue
1
pages
225 - 231
publisher
SPIE
conference name
Optical tomography and spectroscopy of tissue VI, 2005
external identifiers
  • wos:000229735700029
  • scopus:23244458633
ISSN
1996-756X
0277-786X
DOI
10.1117/12.590145
language
English
LU publication?
yes
id
3365e487-15ab-4dfc-8b80-780b7403d758 (old id 615946)
date added to LUP
2007-11-25 17:13:51
date last changed
2017-11-20 15:06:00
@inproceedings{3365e487-15ab-4dfc-8b80-780b7403d758,
  abstract     = {We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the lesion. We have performed Monte Carlo simulations and measurements on an Intralipid phantom in the wavelength range 850 - 1000 nm. By taking the ratio between the signals at the wavelengths 875 and 930 nm the depth of a fluorescing layer could be determined with 0.8 mm accuracy down at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types with various composition. The results indicate that depth estimation of a lesion is possible with no assumptions made about the optical properties for a wide range of tissues},
  author       = {Swartling, Johannes and Bengtsson, D and Terike, K and Svensson, Jenny and Andersson-Engels, Stefan},
  booktitle    = {Proceedings of the SPIE - The International Society for Optical Engineering},
  issn         = {1996-756X},
  keyword      = {intralipid phantom,spectroscopy,photon migration,turbid media,850 to 1000 nm,10 mm,Monte Carlo simulation,fluorophore,tissue optical property,light absorption,light scattering,excitation light irradiation,fluorescence signal measurement,biological tissue,fluorescent lesion,depth estimation,fluorescence spectra},
  language     = {eng},
  number       = {1},
  pages        = {225--231},
  publisher    = {SPIE},
  title        = {Estimation of depth of fluorescing lesions in tissue from changes in fluorescence spectra},
  url          = {http://dx.doi.org/10.1117/12.590145},
  volume       = {5693},
  year         = {2005},
}