Estimation of depth of fluorescing lesions in tissue from changes in fluorescence spectra
(2005) Optical tomography and spectroscopy of tissue VI, 2005 5693(1). p.225-231- Abstract
- We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the... (More)
- We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the lesion. We have performed Monte Carlo simulations and measurements on an Intralipid phantom in the wavelength range 850 - 1000 nm. By taking the ratio between the signals at the wavelengths 875 and 930 nm the depth of a fluorescing layer could be determined with 0.8 mm accuracy down at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types with various composition. The results indicate that depth estimation of a lesion is possible with no assumptions made about the optical properties for a wide range of tissues (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/615946
- author
- Swartling, Johannes LU ; Bengtsson, D ; Terike, K ; Svensson, Jenny LU and Andersson-Engels, Stefan LU
- organization
- publishing date
- 2005
- type
- Chapter in Book/Report/Conference proceeding
- publication status
- published
- subject
- keywords
- intralipid phantom, spectroscopy, photon migration, turbid media, 850 to 1000 nm, 10 mm, Monte Carlo simulation, fluorophore, tissue optical property, light absorption, light scattering, excitation light irradiation, fluorescence signal measurement, biological tissue, fluorescent lesion, depth estimation, fluorescence spectra
- host publication
- Proceedings of the SPIE - The International Society for Optical Engineering
- volume
- 5693
- issue
- 1
- pages
- 225 - 231
- publisher
- SPIE
- conference name
- Optical tomography and spectroscopy of tissue VI, 2005
- conference location
- San Jose, California, United States
- conference dates
- 2005-01-23 - 2005-01-26
- external identifiers
-
- wos:000229735700029
- scopus:23244458633
- ISSN
- 0277-786X
- 1996-756X
- DOI
- 10.1117/12.590145
- language
- English
- LU publication?
- yes
- id
- 3365e487-15ab-4dfc-8b80-780b7403d758 (old id 615946)
- date added to LUP
- 2016-04-01 11:58:50
- date last changed
- 2024-01-08 03:44:34
@inproceedings{3365e487-15ab-4dfc-8b80-780b7403d758, abstract = {{We present a novel method for estimating the depth of a fluorescent lesion in tissue based on measurements of the fluorescence signal in different wavelength bands. The measured fluorescence spectrum following irradiation by excitation light at the surface is a function of several parameters, because the fluorescence light has to pass through tissue with characteristic scattering and absorption properties. Thus, the intrinsic fluorescence spectrum will be altered, in a way determined by the tissue optical properties, the depth of the fluorophore, and also by the geometry of the light irradiation and the detection system. By analyzing the ratio between the signals at two wavelengths we show that it is possible to estimate the depth of the lesion. We have performed Monte Carlo simulations and measurements on an Intralipid phantom in the wavelength range 850 - 1000 nm. By taking the ratio between the signals at the wavelengths 875 and 930 nm the depth of a fluorescing layer could be determined with 0.8 mm accuracy down at least a depth of 10 mm. Monte Carlo simulations were also performed for different tissue types with various composition. The results indicate that depth estimation of a lesion is possible with no assumptions made about the optical properties for a wide range of tissues}}, author = {{Swartling, Johannes and Bengtsson, D and Terike, K and Svensson, Jenny and Andersson-Engels, Stefan}}, booktitle = {{Proceedings of the SPIE - The International Society for Optical Engineering}}, issn = {{0277-786X}}, keywords = {{intralipid phantom; spectroscopy; photon migration; turbid media; 850 to 1000 nm; 10 mm; Monte Carlo simulation; fluorophore; tissue optical property; light absorption; light scattering; excitation light irradiation; fluorescence signal measurement; biological tissue; fluorescent lesion; depth estimation; fluorescence spectra}}, language = {{eng}}, number = {{1}}, pages = {{225--231}}, publisher = {{SPIE}}, title = {{Estimation of depth of fluorescing lesions in tissue from changes in fluorescence spectra}}, url = {{https://lup.lub.lu.se/search/files/2729167/2370816.pdf}}, doi = {{10.1117/12.590145}}, volume = {{5693}}, year = {{2005}}, }