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Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging

Svensson, Jenny LU ; Garofalakis, A; Meyer, H; Forster, Florian LU ; Ripoll, J and Andersson-Engels, Stefan LU (2005) Photon Migration and Diffuse-Light Imaging II In Proceedings of the SPIE - The International Society for Optical Engineering 5859(1). p.1-585906
Abstract
A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a... (More)
A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
2 cm, yellow fluorescence, fluorophores, red fluorescence, phantom measurements, optical tomography, fluorescent light detection, fluorescent probe, deep-lying tumors, fluorescent object, tissue imaging, depth resolution, fluorescence spectroscopy, tissue phantoms
in
Proceedings of the SPIE - The International Society for Optical Engineering
volume
5859
issue
1
pages
1 - 585906
publisher
SPIE
conference name
Photon Migration and Diffuse-Light Imaging II
external identifiers
  • scopus:28844440135
ISSN
0277-786X
1996-756X
DOI
10.1117/12.632826
language
English
LU publication?
yes
id
64e293bd-fb3e-4e38-9b4f-40a1806c949f (old id 616409)
date added to LUP
2007-11-25 17:12:12
date last changed
2017-11-20 15:06:03
@inproceedings{64e293bd-fb3e-4e38-9b4f-40a1806c949f,
  abstract     = {A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object},
  author       = {Svensson, Jenny and Garofalakis, A and Meyer, H and Forster, Florian and Ripoll, J and Andersson-Engels, Stefan},
  booktitle    = {Proceedings of the SPIE - The International Society for Optical Engineering},
  issn         = {0277-786X},
  keyword      = {2 cm,yellow fluorescence,fluorophores,red fluorescence,phantom measurements,optical tomography,fluorescent light detection,fluorescent probe,deep-lying tumors,fluorescent object,tissue imaging,depth resolution,fluorescence spectroscopy,tissue phantoms},
  language     = {eng},
  number       = {1},
  pages        = {1--585906},
  publisher    = {SPIE},
  title        = {Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging},
  url          = {http://dx.doi.org/10.1117/12.632826},
  volume       = {5859},
  year         = {2005},
}