Proteoglycan production in disomic and trisomy 7-carrying human synovial cells.
Eklund, Erik LU ; Broberg, Karin LU
; Westergren-Thorsson, Gunilla
LU
; Bjärdahlen, Anette
LU
; Hedlund, Maria
LU
and Malmström, Anders
LU
(2002)
In Matrix Biology
21(4).
p.325-335
- Abstract
- To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell... (More)
- To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell function, we extended our study to cultures that had been sub-cloned to contain high amounts of trisomy 7-carrying cells. These cell cultures had approximately four times more versican than their disomic counterparts in the cell culture medium, indicating that versican may be a mediator in the processes of joint destructive disorders. To find an explanation for this increase in versican, we investigated the expression/secretion of PDGF-AA and IL-6, cytokines with their genes located to chromosome 7. Indeed, both these cytokines were increased in the cultures with high frequencies of trisomy 7. We then added the two cytokines to cell cultures of disomic synovial cells, but only cells treated with IL-6 displayed an increased amount of versican. Thus, we suggest that the increased amount of versican in cultures of trisomy 7-carrying cells relates to an autocrine loop involving an increased IL-6 production. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/109464
- author
- Eklund, Erik
LU
; Broberg, Karin
LU
; Westergren-Thorsson, Gunilla
LU
; Bjärdahlen, Anette
LU
; Hedlund, Maria
LU
and Malmström, Anders
LU
- organization
- publishing date
- 2002
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Trisomy 7, Versican, IL-6, Decorin, Synovial cells
- in
- Matrix Biology
- volume
- 21
- issue
- 4
- pages
- 325 - 335
- publisher
- Elsevier
- external identifiers
-
- wos:000177237000003
- pmid:12128070
- scopus:0036069645
- ISSN
- 1569-1802
- DOI
- 10.1016/S0945-053X(02)00012-4
- language
- English
- LU publication?
- yes
- id
- 61833325-e006-4dd4-a554-23349b617d24 (old id 109464)
- alternative location
- http://www.ncbi.nlm.nih.gov:80/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=12128070&dopt=Abstract
- date added to LUP
- 2016-04-01 16:41:27
- date last changed
- 2024-01-26 10:36:21
@article{61833325-e006-4dd4-a554-23349b617d24,
abstract = {{To gain further insight into the synthesis and structure of the synovial matrix of joints, we have established cell cultures from synovial specimens and elaborated their production of hyaluronan and proteoglycans. The cultures secreted mainly the small proteoglycan decorin, but also considerable amounts of the related biglycan and the large proteoglycan versican. Only minor amounts of heparan sulfate proteoglycans were found. All cultures also had a high production of hyaluronan, which highlights the important role for normal joint function of these cells. In joint diseases, a common feature is the presence of an extra chromosome 7 (trisomy 7) in the synovial cells. To study the possible consequences of trisomy 7 on the synovial cell function, we extended our study to cultures that had been sub-cloned to contain high amounts of trisomy 7-carrying cells. These cell cultures had approximately four times more versican than their disomic counterparts in the cell culture medium, indicating that versican may be a mediator in the processes of joint destructive disorders. To find an explanation for this increase in versican, we investigated the expression/secretion of PDGF-AA and IL-6, cytokines with their genes located to chromosome 7. Indeed, both these cytokines were increased in the cultures with high frequencies of trisomy 7. We then added the two cytokines to cell cultures of disomic synovial cells, but only cells treated with IL-6 displayed an increased amount of versican. Thus, we suggest that the increased amount of versican in cultures of trisomy 7-carrying cells relates to an autocrine loop involving an increased IL-6 production.}},
author = {{Eklund, Erik and Broberg, Karin and Westergren-Thorsson, Gunilla and Bjärdahlen, Anette and Hedlund, Maria and Malmström, Anders}},
issn = {{1569-1802}},
keywords = {{Trisomy 7; Versican; IL-6; Decorin; Synovial cells}},
language = {{eng}},
number = {{4}},
pages = {{325--335}},
publisher = {{Elsevier}},
series = {{Matrix Biology}},
title = {{Proteoglycan production in disomic and trisomy 7-carrying human synovial cells.}},
url = {{http://dx.doi.org/10.1016/S0945-053X(02)00012-4}},
doi = {{10.1016/S0945-053X(02)00012-4}},
volume = {{21}},
year = {{2002}},
}