Increased interleukin 2 transcription in murine lymphocytes by ciprofloxacin
Riesbeck, Kristian LU
and Forsgren, Arne
LU
(1994)
In Immunopharmacology
27(2).
p.155-164
- Abstract
The fluoroquinolone antibiotic, ciprofloxacin (cipro), induces hyperproduction of interleukin 2 (IL-2) and interferon-γ (IFN-γ) in stimulated human peripheral blood lymphocytes. In this investigation an enhanced and prolonged IL-2 and IL-2 mRNA response was also detected in both stimulated (T cell mitogens or alloantigens) murine splenocytes and in the stimulated murine T cell line EL-4 in the presence of ciprofloxacin (5-80 μg/ml) as compared to control cells without antibiotics. However, in contrast to human lymphocytes, IFN-γ production was inhibited and IFN-γ mRNA levels were unaffected at 24 h and only slightly upregulated at 48 and 72 h of culture in murine splenocytes incubated with cipro (20 μg/ml). EL-4 cells were transfected... (More)
The fluoroquinolone antibiotic, ciprofloxacin (cipro), induces hyperproduction of interleukin 2 (IL-2) and interferon-γ (IFN-γ) in stimulated human peripheral blood lymphocytes. In this investigation an enhanced and prolonged IL-2 and IL-2 mRNA response was also detected in both stimulated (T cell mitogens or alloantigens) murine splenocytes and in the stimulated murine T cell line EL-4 in the presence of ciprofloxacin (5-80 μg/ml) as compared to control cells without antibiotics. However, in contrast to human lymphocytes, IFN-γ production was inhibited and IFN-γ mRNA levels were unaffected at 24 h and only slightly upregulated at 48 and 72 h of culture in murine splenocytes incubated with cipro (20 μg/ml). EL-4 cells were transfected with a plasmid containing the IL-2 promoter and enhancer region linked to the chloramphenicol acetyltransferase (CAT) reporter gene. Analysis of CAT activity revealed that cipro enhanced IL-2 gene induction. In addition, EL-4 cells incubated with ciprofloxacin showed an early peak and more activated nuclear factor of activated T cells (NFAT-1) as compared to control cells without antibiotics. Cipro did not affect the nuclear transcription factors AP-1 or NFIL-2A. Taken together, cipro inhibited IFN-γ synthesis, but enhanced IL-2 production in murine lymphocytes by means of influencing NFAT-1 and causing an increased IL-2 transcription.
(Less)
- author
- Riesbeck, Kristian
LU
and Forsgren, Arne
LU
- organization
- publishing date
- 1994-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Ciprofloxacin, Interferon-γ, Interleukin 2, Murine lymphocyte, Transcription
- in
- Immunopharmacology
- volume
- 27
- issue
- 2
- pages
- 155 - 164
- publisher
- Elsevier
- external identifiers
-
- scopus:0028211374
- pmid:8014029
- ISSN
- 0162-3109
- DOI
- 10.1016/0162-3109(94)90050-7
- language
- English
- LU publication?
- yes
- id
- 63399a05-b2f6-4f2b-b727-66bac5abf9fc
- date added to LUP
- 2019-03-08 15:41:25
- date last changed
- 2024-01-15 15:35:46
@article{63399a05-b2f6-4f2b-b727-66bac5abf9fc,
abstract = {{<p>The fluoroquinolone antibiotic, ciprofloxacin (cipro), induces hyperproduction of interleukin 2 (IL-2) and interferon-γ (IFN-γ) in stimulated human peripheral blood lymphocytes. In this investigation an enhanced and prolonged IL-2 and IL-2 mRNA response was also detected in both stimulated (T cell mitogens or alloantigens) murine splenocytes and in the stimulated murine T cell line EL-4 in the presence of ciprofloxacin (5-80 μg/ml) as compared to control cells without antibiotics. However, in contrast to human lymphocytes, IFN-γ production was inhibited and IFN-γ mRNA levels were unaffected at 24 h and only slightly upregulated at 48 and 72 h of culture in murine splenocytes incubated with cipro (20 μg/ml). EL-4 cells were transfected with a plasmid containing the IL-2 promoter and enhancer region linked to the chloramphenicol acetyltransferase (CAT) reporter gene. Analysis of CAT activity revealed that cipro enhanced IL-2 gene induction. In addition, EL-4 cells incubated with ciprofloxacin showed an early peak and more activated nuclear factor of activated T cells (NFAT-1) as compared to control cells without antibiotics. Cipro did not affect the nuclear transcription factors AP-1 or NFIL-2A. Taken together, cipro inhibited IFN-γ synthesis, but enhanced IL-2 production in murine lymphocytes by means of influencing NFAT-1 and causing an increased IL-2 transcription.</p>}},
author = {{Riesbeck, Kristian and Forsgren, Arne}},
issn = {{0162-3109}},
keywords = {{Ciprofloxacin; Interferon-γ; Interleukin 2; Murine lymphocyte; Transcription}},
language = {{eng}},
month = {{01}},
number = {{2}},
pages = {{155--164}},
publisher = {{Elsevier}},
series = {{Immunopharmacology}},
title = {{Increased interleukin 2 transcription in murine lymphocytes by ciprofloxacin}},
url = {{http://dx.doi.org/10.1016/0162-3109(94)90050-7}},
doi = {{10.1016/0162-3109(94)90050-7}},
volume = {{27}},
year = {{1994}},
}