Advanced

Studies of the separation and characterisation of mixtures of starch and cellulose derivatives by use of chromatography and mass spectrometry

Nilsson, Carina LU ; Asplund, Anna; Schagerlöf, Herje LU ; Melander, Claes LU ; Andersen, Anne; Tjerneld, Folke LU ; Cohen, Arieh LU and Gorton, Lo LU (2007) In Analytical and Bioanalytical Chemistry 387(6). p.2045-2055
Abstract
In this work a method was developed for characterisation of commercially available polymers consisting of mixtures of substituted cellulose and starch. Selective hydrolysis with specific enzymes was used to achieve separation of the two polymers in the mixture. Enzymes hydrolysing (1 -> 4)-alpha-D and (1 -> 6)-alpha-D-glycosidic bonds were used for the starch part and enzymes hydrolysing (1 -> 4)-beta-D-glycosidic bonds for the cellulose part. The hydrolysed fraction was separated from the unhydrolysed fraction and characterised by use of size-exclusion chromatography (SEC), to confirm that enzyme hydrolysis of the different polymers had occurred. High-performance anion-exchange chromatography (HPAEC) was performed to determine... (More)
In this work a method was developed for characterisation of commercially available polymers consisting of mixtures of substituted cellulose and starch. Selective hydrolysis with specific enzymes was used to achieve separation of the two polymers in the mixture. Enzymes hydrolysing (1 -> 4)-alpha-D and (1 -> 6)-alpha-D-glycosidic bonds were used for the starch part and enzymes hydrolysing (1 -> 4)-beta-D-glycosidic bonds for the cellulose part. The hydrolysed fraction was separated from the unhydrolysed fraction and characterised by use of size-exclusion chromatography (SEC), to confirm that enzyme hydrolysis of the different polymers had occurred. High-performance anion-exchange chromatography (HPAEC) was performed to determine the amount of unmodified glucose units (UGU) in the fractions. Electrospray ionisation mass spectrometry (ESIMS) was used for determination of the substituents. All products were converted to monomers by acid hydrolysis to simplify mass spectral identification of the substituents. The monomers were further subjected to acetylation with acetic acid anhydride to facilitate identification of the substituents. By combining the results from the different analytical techniques a picture of the samples was obtained. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
size-exclusion chromatography, anion-exchange chromatography, mass spectrometry, enzyme hydrolysis, carbohydrates
in
Analytical and Bioanalytical Chemistry
volume
387
issue
6
pages
2045 - 2055
publisher
Springer
external identifiers
  • wos:000244684100012
  • scopus:33847672318
ISSN
1618-2642
DOI
10.1007/s00216-006-1094-x
language
English
LU publication?
yes
id
e856e88a-a0cf-405a-908a-13565f6f63c4 (old id 637539)
date added to LUP
2007-11-30 11:02:47
date last changed
2017-04-23 03:26:56
@article{e856e88a-a0cf-405a-908a-13565f6f63c4,
  abstract     = {In this work a method was developed for characterisation of commercially available polymers consisting of mixtures of substituted cellulose and starch. Selective hydrolysis with specific enzymes was used to achieve separation of the two polymers in the mixture. Enzymes hydrolysing (1 -> 4)-alpha-D and (1 -> 6)-alpha-D-glycosidic bonds were used for the starch part and enzymes hydrolysing (1 -> 4)-beta-D-glycosidic bonds for the cellulose part. The hydrolysed fraction was separated from the unhydrolysed fraction and characterised by use of size-exclusion chromatography (SEC), to confirm that enzyme hydrolysis of the different polymers had occurred. High-performance anion-exchange chromatography (HPAEC) was performed to determine the amount of unmodified glucose units (UGU) in the fractions. Electrospray ionisation mass spectrometry (ESIMS) was used for determination of the substituents. All products were converted to monomers by acid hydrolysis to simplify mass spectral identification of the substituents. The monomers were further subjected to acetylation with acetic acid anhydride to facilitate identification of the substituents. By combining the results from the different analytical techniques a picture of the samples was obtained.},
  author       = {Nilsson, Carina and Asplund, Anna and Schagerlöf, Herje and Melander, Claes and Andersen, Anne and Tjerneld, Folke and Cohen, Arieh and Gorton, Lo},
  issn         = {1618-2642},
  keyword      = {size-exclusion chromatography,anion-exchange chromatography,mass spectrometry,enzyme hydrolysis,carbohydrates},
  language     = {eng},
  number       = {6},
  pages        = {2045--2055},
  publisher    = {Springer},
  series       = {Analytical and Bioanalytical Chemistry},
  title        = {Studies of the separation and characterisation of mixtures of starch and cellulose derivatives by use of chromatography and mass spectrometry},
  url          = {http://dx.doi.org/10.1007/s00216-006-1094-x},
  volume       = {387},
  year         = {2007},
}