Advanced

The MAP kinase substrate MKS1 is a regulator of plant defence responces

Andreasson, Erik LU ; Jenkins, Thomas; Brodersen, Peter; Thorgrimsen, Stephan; Petersen, Nikolaj HT; Zhu, Shijiang; Qiu, Jin-Long; Qiu, Jin-Long; Micheelsen, Pernille and Rocher, Anne, et al. (2005) In EMBO Journal 24(14). p.2579-2589
Abstract
Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the... (More)
Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors. (Less)
Please use this url to cite or link to this publication:
author
, et al. (More)
(Less)
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
protein kinase, phosphoserine, disease resistance
in
EMBO Journal
volume
24
issue
14
pages
2579 - 2589
publisher
Oxford University Press
external identifiers
  • scopus:23044508877
ISSN
1460-2075
DOI
10.1038/sj.emboj.7600737
language
English
LU publication?
yes
id
43fb4b47-b4dd-41a4-9ff6-f3e6e918031e (old id 639690)
date added to LUP
2008-01-09 12:41:43
date last changed
2017-10-29 03:31:45
@article{43fb4b47-b4dd-41a4-9ff6-f3e6e918031e,
  abstract     = {Arabidopsis MAP kinase 4 (MPK4) functions as a regulator of pathogen defense responses, because it is required for both repression of salicylic acid (SA)-dependent resistance and for activation of jasmonate (JA)-dependent defense gene expression. To understand MPK4 signaling mechanisms, we used yeast two-hybrid screening to identify the MPK4 substrate MKS1. Analyses of transgenic plants and genome-wide transcript profiling indicated that MKS1 is required for full SA-dependent resistance in mpk4 mutants, and that overexpression of MKS1 in wild-type plants is sufficient to activate SA-dependent resistance, but does not interfere with induction of a defense gene by JA. Further yeast two-hybrid screening revealed that MKS1 interacts with the WRKY transcription factors WRKY25 and WRKY33. WRKY25 and WRKY33 were shown to be in vitro substrates of MPK4, and a wrky33 knockout mutant was found to exhibit increased expression of the SA-related defense gene PR1. MKS1 may therefore contribute to MPK4-regulated defense activation by coupling the kinase to specific WRKY transcription factors.},
  author       = {Andreasson, Erik and Jenkins, Thomas and Brodersen, Peter and Thorgrimsen, Stephan and Petersen, Nikolaj HT and Zhu, Shijiang and Qiu, Jin-Long and Qiu, Jin-Long and Micheelsen, Pernille and Rocher, Anne and Petersen, Morten and Newman, Mari-Anne and Nielsen, Henrik Bjørn and Hirt, Heribert and Somssich, Imre and Mattsson, Ole and Mundy, John},
  issn         = {1460-2075},
  keyword      = {protein kinase,phosphoserine,disease resistance},
  language     = {eng},
  number       = {14},
  pages        = {2579--2589},
  publisher    = {Oxford University Press},
  series       = {EMBO Journal},
  title        = {The MAP kinase substrate MKS1 is a regulator of plant defence responces},
  url          = {http://dx.doi.org/10.1038/sj.emboj.7600737},
  volume       = {24},
  year         = {2005},
}