Genes from oxidative phosphorylation complexes II-V and two dual-function subunits of complex I are transcribed in Viscum album despite absence of the entire mitochondrial holo-complex I
(2022) In Mitochondrion 62. p.1-12- Abstract
Mistletoes (Viscum) and close relatives are unique among flowering plants in having a drastically altered electron transport chain. Lack of complex I genes has previously been reported for the mitochondrial genome, and here we report an almost complete absence of nuclear-encoded complex I genes in the transcriptome of Viscum album. Compared to Arabidopsis with approximately 40 nuclear complex I genes, we recover only transcripts of two dual-function genes: gamma carbonic anhydrase and L-galactono-1,4-lactone dehydrogenase. The complement of genes belonging to complexes II–V of the oxidative phosphorylation pathway appears to be in accordance with other vascular plants. Additionally, transcripts encoding alternative NAD(P)H... (More)
Mistletoes (Viscum) and close relatives are unique among flowering plants in having a drastically altered electron transport chain. Lack of complex I genes has previously been reported for the mitochondrial genome, and here we report an almost complete absence of nuclear-encoded complex I genes in the transcriptome of Viscum album. Compared to Arabidopsis with approximately 40 nuclear complex I genes, we recover only transcripts of two dual-function genes: gamma carbonic anhydrase and L-galactono-1,4-lactone dehydrogenase. The complement of genes belonging to complexes II–V of the oxidative phosphorylation pathway appears to be in accordance with other vascular plants. Additionally, transcripts encoding alternative NAD(P)H dehydrogenases and alternative oxidase were found. Despite sequence divergence, structural modeling suggests that the encoded proteins are structurally conserved. Complex I loss is a special feature in Viscum species and relatives, as all other parasitic flowering plants investigated to date seem to have a complete OXPHOS system. Hence, Viscum offers a unique system for specifically investigating molecular consequences of complex I absence, such as the role of complex I subunits involved in secondary functions.
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- author
- Petersen, Gitte ; Shyama Prasad Rao, R. ; Anderson, Benjamin ; Zervas, Athanasios ; Seberg, Ole ; Rasmusson, Allan G. LU and Max Møller, Ian
- organization
- publishing date
- 2022-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Complex I, Gamma carbonic anhydrase, Gene expression, Mitochondrial processing protease, Mitochondrial respiratory complexes, Oxidative phosphorylation proteins, Structural modeling, Viscum album
- in
- Mitochondrion
- volume
- 62
- pages
- 12 pages
- publisher
- Elsevier
- external identifiers
-
- pmid:34740863
- scopus:85118538061
- ISSN
- 1567-7249
- DOI
- 10.1016/j.mito.2021.10.006
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2021 The Authors
- id
- 64028eef-b53b-49a2-92e1-1e5479a2571a
- date added to LUP
- 2021-11-22 10:38:48
- date last changed
- 2024-04-20 16:12:09
@article{64028eef-b53b-49a2-92e1-1e5479a2571a,
abstract = {{<p>Mistletoes (Viscum) and close relatives are unique among flowering plants in having a drastically altered electron transport chain. Lack of complex I genes has previously been reported for the mitochondrial genome, and here we report an almost complete absence of nuclear-encoded complex I genes in the transcriptome of Viscum album. Compared to Arabidopsis with approximately 40 nuclear complex I genes, we recover only transcripts of two dual-function genes: gamma carbonic anhydrase and L-galactono-1,4-lactone dehydrogenase. The complement of genes belonging to complexes II–V of the oxidative phosphorylation pathway appears to be in accordance with other vascular plants. Additionally, transcripts encoding alternative NAD(P)H dehydrogenases and alternative oxidase were found. Despite sequence divergence, structural modeling suggests that the encoded proteins are structurally conserved. Complex I loss is a special feature in Viscum species and relatives, as all other parasitic flowering plants investigated to date seem to have a complete OXPHOS system. Hence, Viscum offers a unique system for specifically investigating molecular consequences of complex I absence, such as the role of complex I subunits involved in secondary functions.</p>}},
author = {{Petersen, Gitte and Shyama Prasad Rao, R. and Anderson, Benjamin and Zervas, Athanasios and Seberg, Ole and Rasmusson, Allan G. and Max Møller, Ian}},
issn = {{1567-7249}},
keywords = {{Complex I; Gamma carbonic anhydrase; Gene expression; Mitochondrial processing protease; Mitochondrial respiratory complexes; Oxidative phosphorylation proteins; Structural modeling; Viscum album}},
language = {{eng}},
pages = {{1--12}},
publisher = {{Elsevier}},
series = {{Mitochondrion}},
title = {{Genes from oxidative phosphorylation complexes II-V and two dual-function subunits of complex I are transcribed in Viscum album despite absence of the entire mitochondrial holo-complex I}},
url = {{http://dx.doi.org/10.1016/j.mito.2021.10.006}},
doi = {{10.1016/j.mito.2021.10.006}},
volume = {{62}},
year = {{2022}},
}