Natural anti-GBM antibodies from normal human sera recognize alpha 3(IV)NC1 restrictively and recognize the same epitopes as anti-GBM antibodies from patients with anti-GBM disease
Yang, Rui ; Cui, Zhao ; Hellmark, Thomas LU
; Segelmark, Marten
; Zhao, Ming-hui
and Wang, Hai-yan
(2007)
In Clinical Immunology
124(2).
p.207-212
- Abstract
- Anti-GBM disease is a rare autoimmune condition characterized by autoantibodies targeting the alpha 3 chain non-collagen 1 domain of type IV collagen (alpha 3(IV)NC1). Recently, we isolated IgG reacting with alpha 3(IV)NC1 from normal healthy human sera. The current study examined the antigen and epitope specificity of these natural autoantibodies (NAA) using recombinant human alpha 1, 3, 5(IV)NC1 and three constructs expressing, previously defined epitope regions designated E-A, E-B and S2, in the alpha 1(IV)NC1 background. The NAA preparations reacted with recombinant human alpha 3(IV) NC1 to the same extent as with purified bovine alpha(IV)NC1, but not with recombinant human alpha 1 and alpha 5 (IV)NC1. NAA preparations recognized the... (More)
- Anti-GBM disease is a rare autoimmune condition characterized by autoantibodies targeting the alpha 3 chain non-collagen 1 domain of type IV collagen (alpha 3(IV)NC1). Recently, we isolated IgG reacting with alpha 3(IV)NC1 from normal healthy human sera. The current study examined the antigen and epitope specificity of these natural autoantibodies (NAA) using recombinant human alpha 1, 3, 5(IV)NC1 and three constructs expressing, previously defined epitope regions designated E-A, E-B and S2, in the alpha 1(IV)NC1 background. The NAA preparations reacted with recombinant human alpha 3(IV) NC1 to the same extent as with purified bovine alpha(IV)NC1, but not with recombinant human alpha 1 and alpha 5 (IV)NC1. NAA preparations recognized the three chimeric proteins (E-A, E-B and S2) yielding similar absorbance values. We conclude that anti-GBM NAA recognize the same major epitopes as anti-GBM antibodies from patients with Goodpasture's disease. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/647830
- author
- Yang, Rui
; Cui, Zhao
; Hellmark, Thomas
LU
; Segelmark, Marten
; Zhao, Ming-hui
and Wang, Hai-yan
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- natural anti-GBM antibody, antigen specificity, epitope mapping
- in
- Clinical Immunology
- volume
- 124
- issue
- 2
- pages
- 207 - 212
- publisher
- Elsevier
- external identifiers
-
- wos:000248371100012
- scopus:34447544275
- ISSN
- 1521-6616
- DOI
- 10.1016/j.clim.2007.05.001
- language
- English
- LU publication?
- yes
- id
- 16eced75-427e-4ab0-95ec-da08a8354f9e (old id 647830)
- date added to LUP
- 2016-04-01 11:56:48
- date last changed
- 2022-04-05 07:23:04
@article{16eced75-427e-4ab0-95ec-da08a8354f9e,
abstract = {{Anti-GBM disease is a rare autoimmune condition characterized by autoantibodies targeting the alpha 3 chain non-collagen 1 domain of type IV collagen (alpha 3(IV)NC1). Recently, we isolated IgG reacting with alpha 3(IV)NC1 from normal healthy human sera. The current study examined the antigen and epitope specificity of these natural autoantibodies (NAA) using recombinant human alpha 1, 3, 5(IV)NC1 and three constructs expressing, previously defined epitope regions designated E-A, E-B and S2, in the alpha 1(IV)NC1 background. The NAA preparations reacted with recombinant human alpha 3(IV) NC1 to the same extent as with purified bovine alpha(IV)NC1, but not with recombinant human alpha 1 and alpha 5 (IV)NC1. NAA preparations recognized the three chimeric proteins (E-A, E-B and S2) yielding similar absorbance values. We conclude that anti-GBM NAA recognize the same major epitopes as anti-GBM antibodies from patients with Goodpasture's disease.}},
author = {{Yang, Rui and Cui, Zhao and Hellmark, Thomas and Segelmark, Marten and Zhao, Ming-hui and Wang, Hai-yan}},
issn = {{1521-6616}},
keywords = {{natural anti-GBM antibody; antigen specificity; epitope mapping}},
language = {{eng}},
number = {{2}},
pages = {{207--212}},
publisher = {{Elsevier}},
series = {{Clinical Immunology}},
title = {{Natural anti-GBM antibodies from normal human sera recognize alpha 3(IV)NC1 restrictively and recognize the same epitopes as anti-GBM antibodies from patients with anti-GBM disease}},
url = {{http://dx.doi.org/10.1016/j.clim.2007.05.001}},
doi = {{10.1016/j.clim.2007.05.001}},
volume = {{124}},
year = {{2007}},
}