Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Impact of staining on cell acoustic properties

Wang, Qing LU ; Verma, Taru ; Edthofer, Alexander LU ; Baasch, Thierry LU ; Laurell, Thomas LU ; Sand, Karina K. ; Lenshof, Andreas LU orcid and Qiu, Wei LU orcid (2025) In Biophysical Journal
Abstract
Cell staining techniques are essential for cell visualization and bioanalysis, providing valuable insights into cellular structure, function, and behavior. Despite their significance, the impact of staining processes, such as dye penetration, adsorption, or hydrogen bonding, on the physical properties of cells remains largely unexplored. Acoustic methods have proven effective in noninvasively probing various cell properties. In this study, we examine how different staining methods affect the acoustic properties of live DU145, MCF-7, Jurkat, and BV2 cell lines by comparing their acoustic impedance with and without staining. Our results show that calcein staining reduces the acoustic impedance of MCF-7 cells by 2.7%, whereas no noticeable... (More)
Cell staining techniques are essential for cell visualization and bioanalysis, providing valuable insights into cellular structure, function, and behavior. Despite their significance, the impact of staining processes, such as dye penetration, adsorption, or hydrogen bonding, on the physical properties of cells remains largely unexplored. Acoustic methods have proven effective in noninvasively probing various cell properties. In this study, we examine how different staining methods affect the acoustic properties of live DU145, MCF-7, Jurkat, and BV2 cell lines by comparing their acoustic impedance with and without staining. Our results show that calcein staining reduces the acoustic impedance of MCF-7 cells by 2.7%, whereas no noticeable changes are observed in other cases. This reduction, unique to MCF-7 cells, is most consistent with a modest increase in cell compressibility, potentially reflecting subtle alterations in membrane or cytoplasmic mechanics during dye and low-percent DMSO exposure. (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
pages
9 pages
publisher
Cell Press
external identifiers
  • pmid:41215532
ISSN
1542-0086
DOI
10.1016/j.bpj.2025.11.007
language
English
LU publication?
yes
id
649ed2e5-2e85-41ba-ba85-053101aeb0d9
date added to LUP
2025-12-02 23:30:45
date last changed
2025-12-08 13:19:41
@article{649ed2e5-2e85-41ba-ba85-053101aeb0d9,
  abstract     = {{Cell staining techniques are essential for cell visualization and bioanalysis, providing valuable insights into cellular structure, function, and behavior. Despite their significance, the impact of staining processes, such as dye penetration, adsorption, or hydrogen bonding, on the physical properties of cells remains largely unexplored. Acoustic methods have proven effective in noninvasively probing various cell properties. In this study, we examine how different staining methods affect the acoustic properties of live DU145, MCF-7, Jurkat, and BV2 cell lines by comparing their acoustic impedance with and without staining. Our results show that calcein staining reduces the acoustic impedance of MCF-7 cells by 2.7%, whereas no noticeable changes are observed in other cases. This reduction, unique to MCF-7 cells, is most consistent with a modest increase in cell compressibility, potentially reflecting subtle alterations in membrane or cytoplasmic mechanics during dye and low-percent DMSO exposure.}},
  author       = {{Wang, Qing and Verma, Taru and Edthofer, Alexander and Baasch, Thierry and Laurell, Thomas and Sand, Karina K. and Lenshof, Andreas and Qiu, Wei}},
  issn         = {{1542-0086}},
  language     = {{eng}},
  month        = {{11}},
  publisher    = {{Cell Press}},
  series       = {{Biophysical Journal}},
  title        = {{Impact of staining on cell acoustic properties}},
  url          = {{http://dx.doi.org/10.1016/j.bpj.2025.11.007}},
  doi          = {{10.1016/j.bpj.2025.11.007}},
  year         = {{2025}},
}