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Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging

Svensson, Jenny LU ; Garofalakis, A ; Meyer, H ; Forster, Florian LU ; Ripoll, J and Andersson-Engels, Stefan LU (2005) Photon Migration and Diffuse-Light Imaging II 5859(1). p.1-585906
Abstract
A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a... (More)
A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object (Less)
Please use this url to cite or link to this publication:
author
; ; ; ; and
organization
publishing date
type
Chapter in Book/Report/Conference proceeding
publication status
published
subject
keywords
2 cm, yellow fluorescence, fluorophores, red fluorescence, phantom measurements, optical tomography, fluorescent light detection, fluorescent probe, deep-lying tumors, fluorescent object, tissue imaging, depth resolution, fluorescence spectroscopy, tissue phantoms
host publication
Proceedings of the SPIE - The International Society for Optical Engineering
volume
5859
issue
1
pages
1 - 585906
publisher
SPIE
conference name
Photon Migration and Diffuse-Light Imaging II
conference location
Munich, Germany
conference dates
2005-06-12
external identifiers
  • scopus:28844440135
ISSN
0277-786X
1996-756X
DOI
10.1117/12.632826
language
English
LU publication?
yes
id
64e293bd-fb3e-4e38-9b4f-40a1806c949f (old id 616409)
date added to LUP
2016-04-01 12:17:16
date last changed
2024-01-08 15:05:46
@inproceedings{64e293bd-fb3e-4e38-9b4f-40a1806c949f,
  abstract     = {{A way to determine the depth of an embedded fluorescent object, for example deep-lying tumors marked with a fluorescent probe, is to detect fluorescent light that has propagated through the medium at two different wavelength bands. A ratio can then be calculated between the corresponding intensities. The wavelength regions should be chosen such that there is a difference in the absorption in the medium. This spectral information could be used as a complement in other methods, for example in tomography, due to its straightforward implementation. In this study we have performed phantom measurements to determine the depth of a fluorescent object, filled with fluorophores. The transmission of yellow and red fluorescence was measured and a ratio of yellow to red fluorescence was calculated for several depths in tissue with a thickness of 2 cm. The ratio showed a clear dependence on the depth of the object}},
  author       = {{Svensson, Jenny and Garofalakis, A and Meyer, H and Forster, Florian and Ripoll, J and Andersson-Engels, Stefan}},
  booktitle    = {{Proceedings of the SPIE - The International Society for Optical Engineering}},
  issn         = {{0277-786X}},
  keywords     = {{2 cm; yellow fluorescence; fluorophores; red fluorescence; phantom measurements; optical tomography; fluorescent light detection; fluorescent probe; deep-lying tumors; fluorescent object; tissue imaging; depth resolution; fluorescence spectroscopy; tissue phantoms}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{1--585906}},
  publisher    = {{SPIE}},
  title        = {{Fluorescence spectroscopy in tissue phantoms for improved depth resolution in tissue imaging}},
  url          = {{http://dx.doi.org/10.1117/12.632826}},
  doi          = {{10.1117/12.632826}},
  volume       = {{5859}},
  year         = {{2005}},
}