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Long-term neuroblast migration along blood vessels in an area with transient angiogenesis and increased vascularization after stroke

Thored, Pär LU ; Wood, James LU ; Arvidsson, Andreas LU ; Cammenga, Jörg LU ; Kokaia, Zaal LU and Lindvall, Olle LU (2007) In Stroke: a journal of cerebral circulation 38(11). p.3032-3039
Abstract
Background and Purpose - Stroke induced by middle cerebral artery occlusion (MCAO) causes long-term formation of new striatal neurons from stem/progenitor cells in the subventricular zone (SVZ). We explored whether MCAO leads to hypoxia, changes in vessel density, and angiogenesis in the ipsilateral SVZ and adjacent striatum, and determined the relation between the migrating neuroblasts and the vasculature. Methods - Adult rats were subjected to 2 hours of MCAO. Hypoxia was studied by injecting Hypoxyprobe-1 during MCAO or 6 weeks later. Vessel density and length was estimated using stereology. New cells were labeled with 5'-bromo-2' deoxyuridine (BrdU) during weeks 1 and 2 or 7 and 8 after MCAO, and angiogenesis was assessed... (More)
Background and Purpose - Stroke induced by middle cerebral artery occlusion (MCAO) causes long-term formation of new striatal neurons from stem/progenitor cells in the subventricular zone (SVZ). We explored whether MCAO leads to hypoxia, changes in vessel density, and angiogenesis in the ipsilateral SVZ and adjacent striatum, and determined the relation between the migrating neuroblasts and the vasculature. Methods - Adult rats were subjected to 2 hours of MCAO. Hypoxia was studied by injecting Hypoxyprobe-1 during MCAO or 6 weeks later. Vessel density and length was estimated using stereology. New cells were labeled with 5'-bromo-2' deoxyuridine (BrdU) during weeks 1 and 2 or 7 and 8 after MCAO, and angiogenesis was assessed immunohistochemically with antibodies against BrdU and endothelial cell markers. Distance from neuroblasts to nearest vessel was measured using confocal microscopy. Results - The ischemic insult caused transient hypoxia and early, low-grade angiogenesis, but no damage or increase of vascular density in the SVZ. Angiogenesis was detected during the first 2 weeks in the dorsomedial striatum adjacent to the SVZ, which also showed long-lasting increase of vascularization. At 2, 6, and 16 weeks after MCAO, the majority of neuroblasts migrated through this area toward the damage, closely associated with blood vessels. Conclusions - The vasculature plays an important role for long-term striatal neurogenesis after stroke. During several months, neuroblasts migrate close to blood vessels through an area exhibiting early vascular remodeling and persistently increased vessel density. Optimizing vascularization should be an important strategy to promote neurogenesis and repair after stroke. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
hypoxia, neurogenesis, angiogenesis, stroke
in
Stroke: a journal of cerebral circulation
volume
38
issue
11
pages
3032 - 3039
publisher
American Heart Association
external identifiers
  • wos:000250518300026
  • scopus:35648934511
ISSN
1524-4628
DOI
10.1161/STROKEAHA.107.488445
language
English
LU publication?
yes
id
5b379330-b79e-40fc-9f31-d14930ae402b (old id 651766)
date added to LUP
2007-12-19 09:12:03
date last changed
2017-11-12 03:58:34
@article{5b379330-b79e-40fc-9f31-d14930ae402b,
  abstract     = {Background and Purpose - Stroke induced by middle cerebral artery occlusion (MCAO) causes long-term formation of new striatal neurons from stem/progenitor cells in the subventricular zone (SVZ). We explored whether MCAO leads to hypoxia, changes in vessel density, and angiogenesis in the ipsilateral SVZ and adjacent striatum, and determined the relation between the migrating neuroblasts and the vasculature. Methods - Adult rats were subjected to 2 hours of MCAO. Hypoxia was studied by injecting Hypoxyprobe-1 during MCAO or 6 weeks later. Vessel density and length was estimated using stereology. New cells were labeled with 5'-bromo-2' deoxyuridine (BrdU) during weeks 1 and 2 or 7 and 8 after MCAO, and angiogenesis was assessed immunohistochemically with antibodies against BrdU and endothelial cell markers. Distance from neuroblasts to nearest vessel was measured using confocal microscopy. Results - The ischemic insult caused transient hypoxia and early, low-grade angiogenesis, but no damage or increase of vascular density in the SVZ. Angiogenesis was detected during the first 2 weeks in the dorsomedial striatum adjacent to the SVZ, which also showed long-lasting increase of vascularization. At 2, 6, and 16 weeks after MCAO, the majority of neuroblasts migrated through this area toward the damage, closely associated with blood vessels. Conclusions - The vasculature plays an important role for long-term striatal neurogenesis after stroke. During several months, neuroblasts migrate close to blood vessels through an area exhibiting early vascular remodeling and persistently increased vessel density. Optimizing vascularization should be an important strategy to promote neurogenesis and repair after stroke.},
  author       = {Thored, Pär and Wood, James and Arvidsson, Andreas and Cammenga, Jörg and Kokaia, Zaal and Lindvall, Olle},
  issn         = {1524-4628},
  keyword      = {hypoxia,neurogenesis,angiogenesis,stroke},
  language     = {eng},
  number       = {11},
  pages        = {3032--3039},
  publisher    = {American Heart Association},
  series       = { Stroke: a journal of cerebral circulation},
  title        = {Long-term neuroblast migration along blood vessels in an area with transient angiogenesis and increased vascularization after stroke},
  url          = {http://dx.doi.org/10.1161/STROKEAHA.107.488445},
  volume       = {38},
  year         = {2007},
}