Skip to main content

Lund University Publications

LUND UNIVERSITY LIBRARIES

Impaired differentiation of chronic obstructive pulmonary disease bronchial epithelial cells grown on bronchial scaffolds

Hedström, Ulf LU ; Öberg, Lisa ; Vaarala, Outi ; Dellgren, Göran ; Silverborn, Martin ; Bjermer, Leif LU ; Westergren-Thorsson, Gunilla LU ; Hallgren, Oskar LU and Zhou, Xiaohong (2021) In American Journal of Respiratory Cell and Molecular Biology 65(2). p.201-213
Abstract

Chronic obstructive pulmonary disease (COPD) is characterized by airway inflammation, small airway remodeling, and emphysema. Airway remodeling in patients with COPD involves both the airway epithelium and the subepithelial extracellular matrix (ECM). However, it is currently unknown how epithelial remodeling in COPD airways depends on the relative influence from inherent defects in the epithelial cells and alterations in the ECM. To address this, we analyzed global gene expression in COPD human bronchial epithelial cells (HBEC) and normal HBEC after repopulation on decellularized bronchial scaffolds derived from patients with COPD or donors without COPD. COPD HBEC grown on bronchial scaffolds showed an impaired ability to initiate... (More)

Chronic obstructive pulmonary disease (COPD) is characterized by airway inflammation, small airway remodeling, and emphysema. Airway remodeling in patients with COPD involves both the airway epithelium and the subepithelial extracellular matrix (ECM). However, it is currently unknown how epithelial remodeling in COPD airways depends on the relative influence from inherent defects in the epithelial cells and alterations in the ECM. To address this, we analyzed global gene expression in COPD human bronchial epithelial cells (HBEC) and normal HBEC after repopulation on decellularized bronchial scaffolds derived from patients with COPD or donors without COPD. COPD HBEC grown on bronchial scaffolds showed an impaired ability to initiate ciliated-cell differentiation, which was evident on all scaffolds regardless of their origin. In addition, although normal HBEC were less affected by the disease state of the bronchial scaffolds, COPD HBEC showed a gene expression pattern indicating increased proliferation and a retained basal-cell phenotype when grown on COPD bronchial scaffolds compared with normal bronchial scaffolds. By using mass spectrometry, we identified 13 matrisome proteins as being differentially abundant between COPD bronchial scaffolds and normal bronchial scaffolds. These observations are consistent with COPD pathology and suggest that both epithelial cells and the ECM contribute to epithelial-cell remodeling in COPD airways.

(Less)
Please use this url to cite or link to this publication:
author
; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Airway, Chronic obstructive pulmonary disease, Epithelium, Extracellular matrix, Remodeling
in
American Journal of Respiratory Cell and Molecular Biology
volume
65
issue
2
pages
13 pages
publisher
American Thoracic Society
external identifiers
  • pmid:33882260
  • scopus:85111769385
ISSN
1044-1549
DOI
10.1165/rcmb.2019-0395OC
language
English
LU publication?
yes
id
65415b31-d0d3-4f68-abfd-c6229e77e26e
date added to LUP
2021-08-26 15:07:08
date last changed
2024-06-15 15:09:49
@article{65415b31-d0d3-4f68-abfd-c6229e77e26e,
  abstract     = {{<p>Chronic obstructive pulmonary disease (COPD) is characterized by airway inflammation, small airway remodeling, and emphysema. Airway remodeling in patients with COPD involves both the airway epithelium and the subepithelial extracellular matrix (ECM). However, it is currently unknown how epithelial remodeling in COPD airways depends on the relative influence from inherent defects in the epithelial cells and alterations in the ECM. To address this, we analyzed global gene expression in COPD human bronchial epithelial cells (HBEC) and normal HBEC after repopulation on decellularized bronchial scaffolds derived from patients with COPD or donors without COPD. COPD HBEC grown on bronchial scaffolds showed an impaired ability to initiate ciliated-cell differentiation, which was evident on all scaffolds regardless of their origin. In addition, although normal HBEC were less affected by the disease state of the bronchial scaffolds, COPD HBEC showed a gene expression pattern indicating increased proliferation and a retained basal-cell phenotype when grown on COPD bronchial scaffolds compared with normal bronchial scaffolds. By using mass spectrometry, we identified 13 matrisome proteins as being differentially abundant between COPD bronchial scaffolds and normal bronchial scaffolds. These observations are consistent with COPD pathology and suggest that both epithelial cells and the ECM contribute to epithelial-cell remodeling in COPD airways. </p>}},
  author       = {{Hedström, Ulf and Öberg, Lisa and Vaarala, Outi and Dellgren, Göran and Silverborn, Martin and Bjermer, Leif and Westergren-Thorsson, Gunilla and Hallgren, Oskar and Zhou, Xiaohong}},
  issn         = {{1044-1549}},
  keywords     = {{Airway; Chronic obstructive pulmonary disease; Epithelium; Extracellular matrix; Remodeling}},
  language     = {{eng}},
  number       = {{2}},
  pages        = {{201--213}},
  publisher    = {{American Thoracic Society}},
  series       = {{American Journal of Respiratory Cell and Molecular Biology}},
  title        = {{Impaired differentiation of chronic obstructive pulmonary disease bronchial epithelial cells grown on bronchial scaffolds}},
  url          = {{http://dx.doi.org/10.1165/rcmb.2019-0395OC}},
  doi          = {{10.1165/rcmb.2019-0395OC}},
  volume       = {{65}},
  year         = {{2021}},
}