Competition between transglycosylation and hydrolysis in almond beta-glucosidase-catalyzed conversion of p-nitrophenyl-beta-D-glucoside in monophasic water/alcohol mixtures
Mladenoska, Irina LU ; Grey, Carl LU ; Winkelhausen, Eleonora ; Kuzmanova, Slobodanka and Adlercreutz, Patrick LU
(2007)
In Biocatalysis and Biotransformation
25(5).
p.382-385
- Abstract
- Almond beta-glucosidase was used to catalyze the transglycosylation of p-nitrophenyl-beta-D-glucoside to alkyl glucosides, with hydrolysis to glucose as a side reaction. The conversions were carried out in alcohols with varying water contents below water saturation. Both the total reaction rate and the ratio between the transglycosylation and hydrolysis increased with increasing water activity, and at a fixed water activity in the different alcohols, rate and transglycosylation/hydrolysis ratio increased in the following order: 1-octanol < 1-hexanol < 1-butanol. Synthesis of alkyl glucosides by transglycosylation in monophasic alcohol media is thus most favorable for short chain alcohols, and should be carried out at high water... (More)
- Almond beta-glucosidase was used to catalyze the transglycosylation of p-nitrophenyl-beta-D-glucoside to alkyl glucosides, with hydrolysis to glucose as a side reaction. The conversions were carried out in alcohols with varying water contents below water saturation. Both the total reaction rate and the ratio between the transglycosylation and hydrolysis increased with increasing water activity, and at a fixed water activity in the different alcohols, rate and transglycosylation/hydrolysis ratio increased in the following order: 1-octanol < 1-hexanol < 1-butanol. Synthesis of alkyl glucosides by transglycosylation in monophasic alcohol media is thus most favorable for short chain alcohols, and should be carried out at high water content. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/655799
- author
- Mladenoska, Irina
LU
; Grey, Carl
LU
; Winkelhausen, Eleonora
; Kuzmanova, Slobodanka
and Adlercreutz, Patrick
LU
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- almond beta-glucosidase, alkyl, monophasic alcohol media, glucosides, hydrolysis, transglycosylation
- in
- Biocatalysis and Biotransformation
- volume
- 25
- issue
- 5
- pages
- 382 - 385
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000249922800003
- scopus:35148886797
- ISSN
- 1024-2422
- DOI
- 10.1080/10242420701510494
- language
- English
- LU publication?
- yes
- id
- a65cfb1e-1e07-4404-b741-94fdd762a055 (old id 655799)
- date added to LUP
- 2016-04-01 11:38:05
- date last changed
- 2022-02-25 19:02:09
@article{a65cfb1e-1e07-4404-b741-94fdd762a055,
abstract = {{Almond beta-glucosidase was used to catalyze the transglycosylation of p-nitrophenyl-beta-D-glucoside to alkyl glucosides, with hydrolysis to glucose as a side reaction. The conversions were carried out in alcohols with varying water contents below water saturation. Both the total reaction rate and the ratio between the transglycosylation and hydrolysis increased with increasing water activity, and at a fixed water activity in the different alcohols, rate and transglycosylation/hydrolysis ratio increased in the following order: 1-octanol < 1-hexanol < 1-butanol. Synthesis of alkyl glucosides by transglycosylation in monophasic alcohol media is thus most favorable for short chain alcohols, and should be carried out at high water content.}},
author = {{Mladenoska, Irina and Grey, Carl and Winkelhausen, Eleonora and Kuzmanova, Slobodanka and Adlercreutz, Patrick}},
issn = {{1024-2422}},
keywords = {{almond beta-glucosidase; alkyl; monophasic alcohol media; glucosides; hydrolysis; transglycosylation}},
language = {{eng}},
number = {{5}},
pages = {{382--385}},
publisher = {{Taylor & Francis}},
series = {{Biocatalysis and Biotransformation}},
title = {{Competition between transglycosylation and hydrolysis in almond beta-glucosidase-catalyzed conversion of p-nitrophenyl-beta-D-glucoside in monophasic water/alcohol mixtures}},
url = {{http://dx.doi.org/10.1080/10242420701510494}},
doi = {{10.1080/10242420701510494}},
volume = {{25}},
year = {{2007}},
}