Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise
(2007) In Connective Tissue Research 48(5). p.254-262- Abstract
- Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the... (More)
- Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/656676
- author
- Soedersten, Fredrik ; Ekman, Stina ; Niehoff, Anja ; Zaucke, Frank ; Heinegård, Dick LU and Hultenby, Kjell
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- TSP-4, tendon, rodent, COMP, exercise
- in
- Connective Tissue Research
- volume
- 48
- issue
- 5
- pages
- 254 - 262
- publisher
- Taylor & Francis
- external identifiers
-
- wos:000249539800005
- scopus:34648843343
- ISSN
- 1607-8438
- DOI
- 10.1080/03008200701587505
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Connective Tissue Biology (013230151)
- id
- 6ae503e4-0193-444b-933c-4b6d5724d69e (old id 656676)
- date added to LUP
- 2016-04-01 11:59:31
- date last changed
- 2022-01-26 21:11:49
@article{6ae503e4-0193-444b-933c-4b6d5724d69e, abstract = {{Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril.}}, author = {{Soedersten, Fredrik and Ekman, Stina and Niehoff, Anja and Zaucke, Frank and Heinegård, Dick and Hultenby, Kjell}}, issn = {{1607-8438}}, keywords = {{TSP-4; tendon; rodent; COMP; exercise}}, language = {{eng}}, number = {{5}}, pages = {{254--262}}, publisher = {{Taylor & Francis}}, series = {{Connective Tissue Research}}, title = {{Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise}}, url = {{http://dx.doi.org/10.1080/03008200701587505}}, doi = {{10.1080/03008200701587505}}, volume = {{48}}, year = {{2007}}, }