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Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise

Soedersten, Fredrik; Ekman, Stina; Niehoff, Anja; Zaucke, Frank; Heinegård, Dick LU and Hultenby, Kjell (2007) In Connective Tissue Research 48(5). p.254-262
Abstract
Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the... (More)
Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
TSP-4, tendon, rodent, COMP, exercise
in
Connective Tissue Research
volume
48
issue
5
pages
254 - 262
publisher
Taylor & Francis
external identifiers
  • wos:000249539800005
  • scopus:34648843343
ISSN
1607-8438
DOI
10.1080/03008200701587505
language
English
LU publication?
yes
id
6ae503e4-0193-444b-933c-4b6d5724d69e (old id 656676)
date added to LUP
2007-12-14 16:44:01
date last changed
2017-01-01 04:44:03
@article{6ae503e4-0193-444b-933c-4b6d5724d69e,
  abstract     = {Fourteen 3-week-old Sprague-Dawley rats were housed in pairs in standard cages (5 controls) and in individual cages with a running wheel. Four of these rats had run 27-36 km/week (low training-LT) and 5 had run 56-92 km/week (high training-HT). After 4 weeks, the rats were euthanized and Achilles tendons were fixed for electron microscopy. The ultrastructural distribution of cartilage oligomeric matrix protein (COMP) and thrombospondin (TSP)-4 and collagen fibril thickness in two different extracellular compartments were studied. The immunolabeling of COMP decreased with longer running distance and was significantly lower in both the pericellular (p = 0.009) and interterritorial (p = 0.03) compartments of the HT rats compared with the controls. TSP-4 immunolabeling was higher in the pericellular compared with the interterritorial compartments in all rats (p = 0.013) but was not correlated with COMP immunolabeling. No alterations in collagen fibril size were found in relation to running; however, the gold markers representing COMP and TSP-4 were mostly found at the dark bands, representing the gap region of the fibril.},
  author       = {Soedersten, Fredrik and Ekman, Stina and Niehoff, Anja and Zaucke, Frank and Heinegård, Dick and Hultenby, Kjell},
  issn         = {1607-8438},
  keyword      = {TSP-4,tendon,rodent,COMP,exercise},
  language     = {eng},
  number       = {5},
  pages        = {254--262},
  publisher    = {Taylor & Francis},
  series       = {Connective Tissue Research},
  title        = {Ultrastructural immunolocalization of cartilage oligomeric matrix protein, thrombospondin-4, and collagen fibril size in rodent Achilles tendon in relation to exercise},
  url          = {http://dx.doi.org/10.1080/03008200701587505},
  volume       = {48},
  year         = {2007},
}