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Curcumin decreases acid sphingomyelinase activity in colon cancer caco-2 cells

Cheng, Yajun LU ; Kozubek, Agnieszka; Ohlsson, Lena LU ; Sternby, Berit LU and Duan, Rui-Dong LU (2007) In Planta Medica 73(8). p.725-730
Abstract
Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular... (More)
Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular sphingomyelin in the cells. Analysis of the hydrolytic products revealed that the activity was derived from acid sphingomyelinase not from phospholipase D. The curcumin-induced reduction of acid SMase required more than 8 h stimulation. Western blotting showed reduced acid sphingomyelinase protein after curcumin stimulation. The inhibitory effect was more potent in monolayer cells than in polarised cells. No changes of other sphingomyelinases were identified. In the concentrations inhibiting acid sphingomyelinase, curcumin inhibited DNA synthesis and induced cell death. In conclusion, curcumin inhibits acid sphingomyelinase and the effect might be involved in its anti proliferative property against colon cancer cells. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
cell proliferation, curcumin, sphingomyelinase, colon cancer
in
Planta Medica
volume
73
issue
8
pages
725 - 730
publisher
Georg Thieme Verlag
external identifiers
  • wos:000248437500003
  • scopus:34547818199
ISSN
0032-0943
DOI
10.1055/s-2007-981540
language
English
LU publication?
yes
id
2435a289-2aaa-4871-ab77-7bbef6da2a1a (old id 656986)
date added to LUP
2007-12-11 10:55:21
date last changed
2017-01-01 04:21:01
@article{2435a289-2aaa-4871-ab77-7bbef6da2a1a,
  abstract     = {Curcumin has been shown to inhibit cell growth and induce apoptosis in colon cancer cells. The metabolism of sphingomyelin has implications in the development of colon cancer. We examined whether curcumin affects the enzymes that hydrolyse sphingomyelin in Caco-2 cells. The cells were cultured in both monolayer and polarized conditions and stimulated with curcumin. The activities of sphingomyelinases were determined. Sphingomyelin and its hydrolytic products were analysed by thin layer chromatography. The changes of acid sphingomyelinase protein were examined by Western blotting. We found that curcumin reduced the hydrolytic capacity of the cells against choline-labelled sphingomyelin, associated with a mild increase of cellular sphingomyelin in the cells. Analysis of the hydrolytic products revealed that the activity was derived from acid sphingomyelinase not from phospholipase D. The curcumin-induced reduction of acid SMase required more than 8 h stimulation. Western blotting showed reduced acid sphingomyelinase protein after curcumin stimulation. The inhibitory effect was more potent in monolayer cells than in polarised cells. No changes of other sphingomyelinases were identified. In the concentrations inhibiting acid sphingomyelinase, curcumin inhibited DNA synthesis and induced cell death. In conclusion, curcumin inhibits acid sphingomyelinase and the effect might be involved in its anti proliferative property against colon cancer cells.},
  author       = {Cheng, Yajun and Kozubek, Agnieszka and Ohlsson, Lena and Sternby, Berit and Duan, Rui-Dong},
  issn         = {0032-0943},
  keyword      = {cell proliferation,curcumin,sphingomyelinase,colon cancer},
  language     = {eng},
  number       = {8},
  pages        = {725--730},
  publisher    = {Georg Thieme Verlag},
  series       = {Planta Medica},
  title        = {Curcumin decreases acid sphingomyelinase activity in colon cancer caco-2 cells},
  url          = {http://dx.doi.org/10.1055/s-2007-981540},
  volume       = {73},
  year         = {2007},
}