Increased β-cell volume in mice fed a high-fat diet: A dynamic study over 12 months.
(2010) In Islets 2(6). p.12-15- Abstract
- As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained... (More)
- As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive β-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive β-cell expansion as evident by early increased islet β-cell volume and total number of β-cells, whereas individual β-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta cell volume. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/1731627
- author
- Ahrén, Jonatan ; Ahrén, Bo LU and Wierup, Nils LU
- organization
- publishing date
- 2010
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Islets
- volume
- 2
- issue
- 6
- pages
- 12 - 15
- publisher
- Landes Bioscience
- external identifiers
-
- pmid:21099337
- scopus:78751563884
- ISSN
- 1938-2022
- language
- English
- LU publication?
- yes
- additional info
- The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Neuroendocrine Cell Biology (013212008), Medicine (Lund) (013230025)
- id
- 65834e6c-cff6-4c67-a17b-d4f22e6d87fc (old id 1731627)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/21099337?dopt=Abstract
- date added to LUP
- 2016-04-04 08:57:07
- date last changed
- 2024-02-11 04:00:44
@article{65834e6c-cff6-4c67-a17b-d4f22e6d87fc,
abstract = {{As we previously demonstrated, there is an adaptive increase in insulin secretion in insulin resistance in the model of high-fat fed female mice. Since it is assumed that islets also adapt to insulin resistance with β-cell expansion, we have now examined beta volume in this experimental model. Female C57BL/6JBomTac mice were therefore fed a high-fat diet (60% fat from lard) for three, six or twelve months and beta cell volume was estimated as β-cell area per islet, individual β-cell size, and β-cell number per islet. Control animals were fed a normal chow (11% fat). We found that β-cell area per islet and total number of beta cells per islet were increased already after three months of high-fat feeding and that this increase was sustained throughout the twelve month study period. In contrast, individual beta cell size showed a dynamic pattern with a reduction after three months followed by increase after six and twelve months. The number of apoptosis (caspase-3) positive β-cells was reduced after three months, whereas there was no difference in proliferation (Ki-67) positive cells, although these were generally rarely observed. Thus, we conclude that insulin resistance accompanying high-fat feeding in mice is followed by progressive β-cell expansion as evident by early increased islet β-cell volume and total number of β-cells, whereas individual β-cell size showed a dynamic response. The model is also associated with an early reduced apoptosis, which may contribute to the increased beta cell volume.}},
author = {{Ahrén, Jonatan and Ahrén, Bo and Wierup, Nils}},
issn = {{1938-2022}},
language = {{eng}},
number = {{6}},
pages = {{12--15}},
publisher = {{Landes Bioscience}},
series = {{Islets}},
title = {{Increased β-cell volume in mice fed a high-fat diet: A dynamic study over 12 months.}},
url = {{http://www.ncbi.nlm.nih.gov/pubmed/21099337?dopt=Abstract}},
volume = {{2}},
year = {{2010}},
}