GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination
(2007) In Diabetic Medicine 24(5). p.521-526- Abstract
- Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level... (More)
- Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/663643
- author
- Bekris, L. M.
; Jensen, R. A.
; Lagerquist, E.
; Hall, T. R.
; Agardh, Carl-David
LU
; Cilio, Corrado
LU
; Lethagen, ÅsaLinda
LU
; Lernmark, Åke
LU
; Robertson, J. A. and Hampe, C. S.
- organization
- publishing date
- 2007
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- autoimmunity, therapy, Type 1 diabetes
- in
- Diabetic Medicine
- volume
- 24
- issue
- 5
- pages
- 521 - 526
- publisher
- Wiley-Blackwell
- external identifiers
-
- wos:000246029400010
- scopus:34247574292
- pmid:17367313
- ISSN
- 1464-5491
- DOI
- 10.1111/j.1464-5491.2007.02091.x
- language
- English
- LU publication?
- yes
- id
- 7d10d2f0-4796-4712-99f4-4c3f7a6c53a8 (old id 663643)
- date added to LUP
- 2016-04-01 16:31:36
- date last changed
- 2024-03-13 08:30:34
@article{7d10d2f0-4796-4712-99f4-4c3f7a6c53a8, abstract = {{Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern.}}, author = {{Bekris, L. M. and Jensen, R. A. and Lagerquist, E. and Hall, T. R. and Agardh, Carl-David and Cilio, Corrado and Lethagen, ÅsaLinda and Lernmark, Åke and Robertson, J. A. and Hampe, C. S.}}, issn = {{1464-5491}}, keywords = {{autoimmunity; therapy; Type 1 diabetes}}, language = {{eng}}, number = {{5}}, pages = {{521--526}}, publisher = {{Wiley-Blackwell}}, series = {{Diabetic Medicine}}, title = {{GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination}}, url = {{http://dx.doi.org/10.1111/j.1464-5491.2007.02091.x}}, doi = {{10.1111/j.1464-5491.2007.02091.x}}, volume = {{24}}, year = {{2007}}, }