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GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination

Bekris, L. M.; Jensen, R. A.; Lagerquist, E.; Hall, T. R.; Agardh, Carl-David LU ; Cilio, Corrado LU ; Lethagen, ÅsaLinda LU ; Lernmark, Åke LU ; Robertson, J. A. and Hampe, C. S. (2007) In Diabetic Medicine 24(5). p.521-526
Abstract
Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level... (More)
Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
autoimmunity, therapy, Type 1 diabetes
in
Diabetic Medicine
volume
24
issue
5
pages
521 - 526
publisher
Wiley-Blackwell
external identifiers
  • wos:000246029400010
  • scopus:34247574292
ISSN
1464-5491
DOI
10.1111/j.1464-5491.2007.02091.x
language
English
LU publication?
yes
id
7d10d2f0-4796-4712-99f4-4c3f7a6c53a8 (old id 663643)
date added to LUP
2007-12-07 15:08:05
date last changed
2017-01-01 07:07:46
@article{7d10d2f0-4796-4712-99f4-4c3f7a6c53a8,
  abstract     = {Aims Subcutaneous injection of recombinant human GAD65 (rhGAD65) in patients with latent autoimmune diabetes in adults (LADA) correlates with an increase in C-peptide levels. In this study we analysed the effect of rhGAD65 administration on the GAD65-specific autoimmune response. Methods Longitudinal serum samples obtained from LADA patients (n = 47) who received 4, 20, 100 or 500 mu g alum-formulated rhGAD65 or placebo by subcutaneous injection twice (4 weeks apart) were analysed for their epitope recognition using GAD65-specific recombinant Fab and GAD65/67 fusion proteins. Results Overall, minor changes in the epitope pattern were observed using either approach. Only in the 500-mu g dosage group was an increase in GAD65Ab level associated with a significant increase in the binding to a conformational epitope located at the middle part of GAD65. Conclusions Our data suggest that the apparent beneficial effects of 20 mu g alum-formulated recombinant human GAD65 is not explained by changes in the GAD65Ab epitope pattern.},
  author       = {Bekris, L. M. and Jensen, R. A. and Lagerquist, E. and Hall, T. R. and Agardh, Carl-David and Cilio, Corrado and Lethagen, ÅsaLinda and Lernmark, Åke and Robertson, J. A. and Hampe, C. S.},
  issn         = {1464-5491},
  keyword      = {autoimmunity,therapy,Type 1 diabetes},
  language     = {eng},
  number       = {5},
  pages        = {521--526},
  publisher    = {Wiley-Blackwell},
  series       = {Diabetic Medicine},
  title        = {GAD65 autoantibody epitopes in adult patients with latent autoimmune diabetes following GAD65 vaccination},
  url          = {http://dx.doi.org/10.1111/j.1464-5491.2007.02091.x},
  volume       = {24},
  year         = {2007},
}