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Microvesicles in plasma reflect coronary flow reserve in patients with cardiovascular disease

Bryl-Górecka, Paulina LU ; James, Kreema LU orcid ; Torngren, Kristina LU ; Haraldsson, Inger ; Gan, Li Ming ; Svedlund, Sara ; Olde, Björn LU ; Laurell, Thomas LU ; Omerovic, Elmir and Erlinge, David LU orcid (2021) In American Journal of Physiology - Heart and Circulatory Physiology 320(5). p.2147-2160
Abstract

High levels of microvesicles (MVs), a type of extracellular vesicles, are detected in several pathological conditions. We investigated the connection between coronary flow reserve (CFR), a prognostic clinical parameter that reflects blood flow in the heart, with levels of MVs and their cargo, from plasma of patients with cardiovascular disease. The PROFLOW study consists of 220 patients with prior myocardial infarction and measured CFR with transthoracic echocardiography. The patients were divided into high and low CFR groups. Plasma MVs were captured with acoustic trapping. Platelet- and endothelial-derived MVs were measured with flow cytometry, and vesicle lysates were analyzed with proteomic panels against cardiovascular biomarkers.... (More)

High levels of microvesicles (MVs), a type of extracellular vesicles, are detected in several pathological conditions. We investigated the connection between coronary flow reserve (CFR), a prognostic clinical parameter that reflects blood flow in the heart, with levels of MVs and their cargo, from plasma of patients with cardiovascular disease. The PROFLOW study consists of 220 patients with prior myocardial infarction and measured CFR with transthoracic echocardiography. The patients were divided into high and low CFR groups. Plasma MVs were captured with acoustic trapping. Platelet- and endothelial-derived MVs were measured with flow cytometry, and vesicle lysates were analyzed with proteomic panels against cardiovascular biomarkers. Flow cytometry was further applied to identify cellular origin of biomarkers. Our data show a negative correlation between MV concentration and CFR values. Platelet and endothelial MV levels were significantly increased in plasma from the low CFR group. CFR negatively correlates with the levels of several proteomic biomarkers, and the low CFR group exhibited higher concentrations of these proteins in MVs. Focused analysis of one of the MV proteins, B cell activating factor (BAFF), revealed platelet and not leukocyte origin and release upon proinflammatory stimulus. Higher levels of MVs carrying an elevated concentration of proatherogenic proteins circulate in plasma in patients with low CFR, a marker of vascular dysfunction, reduced blood flow, and poor prognosis. Our findings demonstrate a potential clinical value of MVs as biomarkers and possible therapeutic targets against endothelial deterioration.

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author
; ; ; ; ; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Atherosclerosis, Biomarkers, Coronary flow reserve, Microvesicles
in
American Journal of Physiology - Heart and Circulatory Physiology
volume
320
issue
5
pages
2147 - 2160
publisher
American Physiological Society
external identifiers
  • scopus:85106554765
  • pmid:33797274
ISSN
0363-6135
DOI
10.1152/AJPHEART.00869.2020
language
English
LU publication?
yes
id
6714de04-84bb-44b1-ba5e-aec960f379ed
date added to LUP
2021-06-09 11:08:49
date last changed
2023-11-23 03:42:53
@article{6714de04-84bb-44b1-ba5e-aec960f379ed,
  abstract     = {{<p>High levels of microvesicles (MVs), a type of extracellular vesicles, are detected in several pathological conditions. We investigated the connection between coronary flow reserve (CFR), a prognostic clinical parameter that reflects blood flow in the heart, with levels of MVs and their cargo, from plasma of patients with cardiovascular disease. The PROFLOW study consists of 220 patients with prior myocardial infarction and measured CFR with transthoracic echocardiography. The patients were divided into high and low CFR groups. Plasma MVs were captured with acoustic trapping. Platelet- and endothelial-derived MVs were measured with flow cytometry, and vesicle lysates were analyzed with proteomic panels against cardiovascular biomarkers. Flow cytometry was further applied to identify cellular origin of biomarkers. Our data show a negative correlation between MV concentration and CFR values. Platelet and endothelial MV levels were significantly increased in plasma from the low CFR group. CFR negatively correlates with the levels of several proteomic biomarkers, and the low CFR group exhibited higher concentrations of these proteins in MVs. Focused analysis of one of the MV proteins, B cell activating factor (BAFF), revealed platelet and not leukocyte origin and release upon proinflammatory stimulus. Higher levels of MVs carrying an elevated concentration of proatherogenic proteins circulate in plasma in patients with low CFR, a marker of vascular dysfunction, reduced blood flow, and poor prognosis. Our findings demonstrate a potential clinical value of MVs as biomarkers and possible therapeutic targets against endothelial deterioration.</p>}},
  author       = {{Bryl-Górecka, Paulina and James, Kreema and Torngren, Kristina and Haraldsson, Inger and Gan, Li Ming and Svedlund, Sara and Olde, Björn and Laurell, Thomas and Omerovic, Elmir and Erlinge, David}},
  issn         = {{0363-6135}},
  keywords     = {{Atherosclerosis; Biomarkers; Coronary flow reserve; Microvesicles}},
  language     = {{eng}},
  number       = {{5}},
  pages        = {{2147--2160}},
  publisher    = {{American Physiological Society}},
  series       = {{American Journal of Physiology - Heart and Circulatory Physiology}},
  title        = {{Microvesicles in plasma reflect coronary flow reserve in patients with cardiovascular disease}},
  url          = {{http://dx.doi.org/10.1152/AJPHEART.00869.2020}},
  doi          = {{10.1152/AJPHEART.00869.2020}},
  volume       = {{320}},
  year         = {{2021}},
}