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The IgD C(H)1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID.

Samuelsson, Martin LU ; Jendholm, Johan LU ; Amisten, Stefan LU ; Morrison, Sherie L; Forsgren, Arne LU and Riesbeck, Kristian LU (2006) In European Journal of Immunology 9(Sep;36). p.2525-2534
Abstract
The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody... (More)
The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody structure. When amino acids 157-224 of the IgD C(H)1 region were substituted with IgG sequences, binding by M. catarrhalis or recombinant MID962-1200 was abolished. Subsequent smaller substitutions of IgD C(H)1 157-224 with IgG sequences led us to conclude that IgD C(H)1 amino acids 198-206 were crucial for the interaction between MID and IgD. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Moraxella catarrhalis, immunoglobulin D, binding
in
European Journal of Immunology
volume
9
issue
Sep;36
pages
2525 - 2534
publisher
John Wiley & Sons
external identifiers
  • wos:000240586100026
  • pmid:16906531
  • scopus:33748857116
ISSN
1521-4141
DOI
10.1002/eji.200535594
language
English
LU publication?
yes
id
67546171-00d8-4c30-9cda-60dbdbea8c11 (old id 160110)
alternative location
http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16906531&dopt=Abstract
date added to LUP
2007-07-27 08:27:52
date last changed
2019-01-06 06:37:32
@article{67546171-00d8-4c30-9cda-60dbdbea8c11,
  abstract     = {The Moraxella catarrhalis IgD-binding protein (MID) has a unique specificity for human IgD, and the sequence with maximal IgD binding is located within the amino acids MID962-1200. In the present paper, we examined the MID binding site on IgD using a series of recombinant Ig. Full-length IgD, IgD F(ab')(2), and an IgD F(ab') C290R mutant lacking the inter-heavy-chain cysteine 290 were manufactured. Furthermore, a series of IgD/IgG chimeras were constructed. ELISA, dot blot and flow cytometry were used to study the binding of purified Ig to native MID, recombinant MID912-1200 or to Moraxella with or without MID. MID962-1200 bound both the IgD F(ab')(2) and F(ab') C290R, indicating that the binding occurred independently of antibody structure. When amino acids 157-224 of the IgD C(H)1 region were substituted with IgG sequences, binding by M. catarrhalis or recombinant MID962-1200 was abolished. Subsequent smaller substitutions of IgD C(H)1 157-224 with IgG sequences led us to conclude that IgD C(H)1 amino acids 198-206 were crucial for the interaction between MID and IgD.},
  author       = {Samuelsson, Martin and Jendholm, Johan and Amisten, Stefan and Morrison, Sherie L and Forsgren, Arne and Riesbeck, Kristian},
  issn         = {1521-4141},
  keyword      = {Moraxella catarrhalis,immunoglobulin D,binding},
  language     = {eng},
  number       = {Sep;36},
  pages        = {2525--2534},
  publisher    = {John Wiley & Sons},
  series       = {European Journal of Immunology},
  title        = {The IgD C(H)1 region contains the binding site for the human respiratory pathogen Moraxella catarrhalis IgD-binding protein MID.},
  url          = {http://dx.doi.org/10.1002/eji.200535594},
  volume       = {9},
  year         = {2006},
}