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Decellularization of the Murine Cardiopulmonary Complex

Mayorca-guiliani, Alejandro E ; Rafaeva, Maria ; Willacy, Oliver ; Madsen, Chris LU ; Reuten, Raphael and Erler, Janine T. (2021) In Journal of visualized experiments : JoVE 171.
Abstract
We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.

The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to... (More)
We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.

The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease. (Less)
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author
; ; ; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Journal of visualized experiments : JoVE
volume
171
publisher
JoVE
external identifiers
  • scopus:85108189346
  • pmid:34125099
ISSN
1940-087X
DOI
10.3791/61854
language
English
LU publication?
yes
id
68b460c4-2ce4-4adf-8117-d51c2674cf2d
date added to LUP
2021-05-31 08:07:34
date last changed
2022-08-02 08:08:16
@article{68b460c4-2ce4-4adf-8117-d51c2674cf2d,
  abstract     = {{We present here a decellularization protocol for mouse heart and lungs. It produces structural ECM scaffolds that can be used to analyze ECM topology and composition. It is based on a microsurgical procedure designed to catheterize the trachea and aorta of a euthanized mouse to perfuse the heart and lungs with decellularizing agents. The decellularized cardiopulmonary complex can subsequently be immunostained to reveal the location of structural ECM proteins. The whole procedure can be completed in 4 days.<br/><br/>The ECM scaffolds resulting from this protocol are free of dimensional distortions. The absence of cells enables structural examination of ECM structures down to submicron resolution in 3D. This protocol can be applied to healthy and diseased tissue from mice as young as 4-weeks old, including mouse models of fibrosis and cancer, opening the way to determine ECM remodeling associated with cardiopulmonary disease.}},
  author       = {{Mayorca-guiliani, Alejandro E and Rafaeva, Maria and Willacy, Oliver and Madsen, Chris and Reuten, Raphael and Erler, Janine T.}},
  issn         = {{1940-087X}},
  language     = {{eng}},
  month        = {{05}},
  publisher    = {{JoVE}},
  series       = {{Journal of visualized experiments : JoVE}},
  title        = {{Decellularization of the Murine Cardiopulmonary Complex}},
  url          = {{http://dx.doi.org/10.3791/61854}},
  doi          = {{10.3791/61854}},
  volume       = {{171}},
  year         = {{2021}},
}