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The fungal metabolite galiellalactone interferes with the nuclear import of NF-κB and inhibits HIV-1 replication.

Pérez, Moisés ; Soler-Torronteras, Rafael ; Collado, Juan A ; Limones, Carmen G ; Hellsten, Rebecka LU ; Johansson, Martin H LU ; Sterner, Olov ; Bjartell, Anders LU ; Calzado, Marco A and Muñoz, Eduardo (2014) In Chemico-Biological Interactions 214(Mar 11). p.69-76
Abstract
Galiellalactone (GL) is a metabolite produced by the fungus Galiella rufa that presents antitumor and immunomodulatory activities. GL interferes with the binding to DNA of signal transducer and activator of transcription (STAT)-3 and also inhibits other signal pathways such as NF-κB, but the mechanism of action in this pathway remains unknown. In this study we report that GL inhibits vesicular stomatitis virus-recombinant HIV-1 infection and the NF-κB-dependent transcriptional activity of the HIV-LTR promoter. We found that GL prevents the binding of NF-κB to DNA but neither affects the phosphorylation and degradation of NF-κB inhibitory protein, IκBα, nor the phosphorylation and acetylation of the NF-κB p65 subunit. However, GL prevents... (More)
Galiellalactone (GL) is a metabolite produced by the fungus Galiella rufa that presents antitumor and immunomodulatory activities. GL interferes with the binding to DNA of signal transducer and activator of transcription (STAT)-3 and also inhibits other signal pathways such as NF-κB, but the mechanism of action in this pathway remains unknown. In this study we report that GL inhibits vesicular stomatitis virus-recombinant HIV-1 infection and the NF-κB-dependent transcriptional activity of the HIV-LTR promoter. We found that GL prevents the binding of NF-κB to DNA but neither affects the phosphorylation and degradation of NF-κB inhibitory protein, IκBα, nor the phosphorylation and acetylation of the NF-κB p65 subunit. However, GL prevents the association of p65 with the importin α3 impairing the nuclear translocation of this transcription factor. Using a biotinylated probe we found that GL binds to p65 but not to importin α3. Therefore, GL is a dual NF-κB/STAT3 inhibitor that could serve as a lead compound for the development of novel drugs against HIV-1, cancer and inflammatory diseases. (Less)
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author
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organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Chemico-Biological Interactions
volume
214
issue
Mar 11
pages
69 - 76
publisher
Elsevier
external identifiers
  • pmid:24631022
  • wos:000335705800009
  • scopus:84896475169
  • pmid:24631022
ISSN
1872-7786
DOI
10.1016/j.cbi.2014.02.012
language
English
LU publication?
yes
id
69090608-f13c-47a1-a7d3-77544d5eed44 (old id 4383220)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/24631022?dopt=Abstract
date added to LUP
2016-04-01 09:53:37
date last changed
2022-02-09 20:42:38
@article{69090608-f13c-47a1-a7d3-77544d5eed44,
  abstract     = {{Galiellalactone (GL) is a metabolite produced by the fungus Galiella rufa that presents antitumor and immunomodulatory activities. GL interferes with the binding to DNA of signal transducer and activator of transcription (STAT)-3 and also inhibits other signal pathways such as NF-κB, but the mechanism of action in this pathway remains unknown. In this study we report that GL inhibits vesicular stomatitis virus-recombinant HIV-1 infection and the NF-κB-dependent transcriptional activity of the HIV-LTR promoter. We found that GL prevents the binding of NF-κB to DNA but neither affects the phosphorylation and degradation of NF-κB inhibitory protein, IκBα, nor the phosphorylation and acetylation of the NF-κB p65 subunit. However, GL prevents the association of p65 with the importin α3 impairing the nuclear translocation of this transcription factor. Using a biotinylated probe we found that GL binds to p65 but not to importin α3. Therefore, GL is a dual NF-κB/STAT3 inhibitor that could serve as a lead compound for the development of novel drugs against HIV-1, cancer and inflammatory diseases.}},
  author       = {{Pérez, Moisés and Soler-Torronteras, Rafael and Collado, Juan A and Limones, Carmen G and Hellsten, Rebecka and Johansson, Martin H and Sterner, Olov and Bjartell, Anders and Calzado, Marco A and Muñoz, Eduardo}},
  issn         = {{1872-7786}},
  language     = {{eng}},
  number       = {{Mar 11}},
  pages        = {{69--76}},
  publisher    = {{Elsevier}},
  series       = {{Chemico-Biological Interactions}},
  title        = {{The fungal metabolite galiellalactone interferes with the nuclear import of NF-κB and inhibits HIV-1 replication.}},
  url          = {{http://dx.doi.org/10.1016/j.cbi.2014.02.012}},
  doi          = {{10.1016/j.cbi.2014.02.012}},
  volume       = {{214}},
  year         = {{2014}},
}