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The secreted serine protease xHtrA1 stimulates long-range FGF signaling in the early Xenopus embryo

Hou, Shirui LU ; Maccarana, Marco LU ; Tan Grahn, Hooi Min LU ; Strate, Ina LU and Pera, Edgar LU (2007) In Developmental Cell 13(2). p.226-241
Abstract
We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the... (More)
We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGIF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cellsurface-bound FGF ligands and stimulates long-range FGF signaling. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Developmental Cell
volume
13
issue
2
pages
226 - 241
publisher
Cell Press
external identifiers
  • wos:000248664300010
  • scopus:34547475712
ISSN
1534-5807
DOI
10.1016/j.devcel.2007.07.001
language
English
LU publication?
yes
id
7bcce079-e86e-4cf5-8034-8204fcee06a4 (old id 692758)
date added to LUP
2007-12-12 16:14:40
date last changed
2017-01-01 04:29:36
@article{7bcce079-e86e-4cf5-8034-8204fcee06a4,
  abstract     = {We found that the secreted serine protease xHtrA1, expressed in the early embryo and transcriptionally activated by FGF signals, promotes posterior development in mRNA-injected Xenopus embryos. xHtrA1 mRNA led to the induction of secondary tail-like structures, expansion of mesoderm, and formation of ectopic neurons in an FGF-dependent manner. An antisense morpholino oligonucleotide or a neutralizing antibody against xHtrA1 had the opposite effects. xHtrA1 activates FGF/ ERK signaling and the transcription of FGF genes. We show that Xenopus Biglycan, Syndecan-4, and Glypican-4 are proteolytic targets of xHtrA1 and that heparan sulfate and dermatan sulfate trigger posteriorization, mesoderm induction, and neuronal differentiation via the FGIF signaling pathway. The results are consistent with a mechanism by which xHtrA1, through cleaving proteoglycans, releases cellsurface-bound FGF ligands and stimulates long-range FGF signaling.},
  author       = {Hou, Shirui and Maccarana, Marco and Tan Grahn, Hooi Min and Strate, Ina and Pera, Edgar},
  issn         = {1534-5807},
  language     = {eng},
  number       = {2},
  pages        = {226--241},
  publisher    = {Cell Press},
  series       = {Developmental Cell},
  title        = {The secreted serine protease xHtrA1 stimulates long-range FGF signaling in the early Xenopus embryo},
  url          = {http://dx.doi.org/10.1016/j.devcel.2007.07.001},
  volume       = {13},
  year         = {2007},
}