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Impact of Individual Process Parameters on Extraction of Polysaccharides from Saccharina latissima

Khajavi Ahmadi, Elmira ; Al-Hamimi, Said LU ; Jönsson, Madeleine and Sardari, Roya R. R. LU (2025) In Marine Drugs 23(11).
Abstract
While numerous extraction methods have been applied to the brown algae Saccharina latissima, a systematic evaluation of how individual extraction parameters influence the extraction of each target polysaccharide has not previously been reported. Accordingly, this study compared conventional and advanced techniques for extracting fucoidan, laminarin, and alginate from pre-treated biomass. Conventional methods employed diluted acid (0.01 M and 0.1 M HCl), diluted alkali (0.01 M and 0.1 M NaOH), and hot water (121 °C for 30/60 min) for extraction. Advanced techniques involved pressurized liquid extraction (PLE) using water and moderate electric field (MEF) extraction with conditions optimized by statistical experimental design. Pre-treatment... (More)
While numerous extraction methods have been applied to the brown algae Saccharina latissima, a systematic evaluation of how individual extraction parameters influence the extraction of each target polysaccharide has not previously been reported. Accordingly, this study compared conventional and advanced techniques for extracting fucoidan, laminarin, and alginate from pre-treated biomass. Conventional methods employed diluted acid (0.01 M and 0.1 M HCl), diluted alkali (0.01 M and 0.1 M NaOH), and hot water (121 °C for 30/60 min) for extraction. Advanced techniques involved pressurized liquid extraction (PLE) using water and moderate electric field (MEF) extraction with conditions optimized by statistical experimental design. Pre-treatment with aqueous ethanol removed 30% ash and eliminated mannitol, improving extraction selectivity. The results demonstrated fucoidan yields of 31% with 0.01 M HCl and 46% with 0.1 M NaOH, while 0.01 M NaOH facilitated laminarin co-extraction (45%). Alginate, as a mannuronic acid polymer, was obtained at 9% yield with 0.1 M HCl, 42% yield with 0.1 M NaOH, and 27% with pressurized hot water for 30 min. High-temperature, short-duration PLE further improved alginate yield, while MEF showed limited gains due to high ionic content but demonstrated potential under optimized settings. The results support a cascading biorefinery approach in which different polysaccharide fractions can be sequentially obtained, contributing to more sustainable seaweed valorization. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Marine Drugs
volume
23
issue
11
article number
435
pages
18 pages
publisher
MDPI AG
external identifiers
  • scopus:105022824347
  • pmid:41295403
ISSN
1660-3397
DOI
10.3390/md23110435
language
English
LU publication?
yes
id
695a943c-8cd5-4a27-9f2d-305a736b247b
date added to LUP
2025-12-20 14:10:22
date last changed
2025-12-23 03:00:08
@article{695a943c-8cd5-4a27-9f2d-305a736b247b,
  abstract     = {{While numerous extraction methods have been applied to the brown algae Saccharina latissima, a systematic evaluation of how individual extraction parameters influence the extraction of each target polysaccharide has not previously been reported. Accordingly, this study compared conventional and advanced techniques for extracting fucoidan, laminarin, and alginate from pre-treated biomass. Conventional methods employed diluted acid (0.01 M and 0.1 M HCl), diluted alkali (0.01 M and 0.1 M NaOH), and hot water (121 °C for 30/60 min) for extraction. Advanced techniques involved pressurized liquid extraction (PLE) using water and moderate electric field (MEF) extraction with conditions optimized by statistical experimental design. Pre-treatment with aqueous ethanol removed 30% ash and eliminated mannitol, improving extraction selectivity. The results demonstrated fucoidan yields of 31% with 0.01 M HCl and 46% with 0.1 M NaOH, while 0.01 M NaOH facilitated laminarin co-extraction (45%). Alginate, as a mannuronic acid polymer, was obtained at 9% yield with 0.1 M HCl, 42% yield with 0.1 M NaOH, and 27% with pressurized hot water for 30 min. High-temperature, short-duration PLE further improved alginate yield, while MEF showed limited gains due to high ionic content but demonstrated potential under optimized settings. The results support a cascading biorefinery approach in which different polysaccharide fractions can be sequentially obtained, contributing to more sustainable seaweed valorization.}},
  author       = {{Khajavi Ahmadi, Elmira and Al-Hamimi, Said and Jönsson, Madeleine and Sardari, Roya R. R.}},
  issn         = {{1660-3397}},
  language     = {{eng}},
  number       = {{11}},
  publisher    = {{MDPI AG}},
  series       = {{Marine Drugs}},
  title        = {{Impact of Individual Process Parameters on Extraction of Polysaccharides from <i>Saccharina latissima</i>}},
  url          = {{http://dx.doi.org/10.3390/md23110435}},
  doi          = {{10.3390/md23110435}},
  volume       = {{23}},
  year         = {{2025}},
}