Advanced

Effect of P availability on temporal dynamics of carbon allocation and Glomus intraradices high-affinity P transporter gene induction in arbuscular mycorrhiza

Olsson, Pål Axel LU ; Hansson, Maria LU and Burleigh, Stephen LU (2006) In Applied and Environmental Microbiology 72(6). p.4115-4120
Abstract
Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but... (More)
Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but we found that C How to the fungus was initially highest at the high P level. Only at later harvests, after 100 days of in vitro. culture, were C flow and fungal growth limited at high P availability. Thus, AM fungi can benefit initially from P-enriched environments in terms of plant C allocation. As expected, the P transporter induction was significantly greater at low P availability and greatest in very young mycelia. We found no direct link between C How to the fungus and the P transporter transcription level, which indicates that a good C supply is not essential for induction of the high-affinity P transporter. We describe a mechanism by which P regulates symbiotic C allocation, and we discuss how this mechanism may have evolved in a competitive environment. (Less)
Please use this url to cite or link to this publication:
author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Applied and Environmental Microbiology
volume
72
issue
6
pages
4115 - 4120
publisher
American Society for Microbiology
external identifiers
  • wos:000238620100039
  • pmid:16751522
  • scopus:33745159330
ISSN
0099-2240
DOI
10.1128/AEM.02154-05
language
English
LU publication?
yes
id
69d69ae1-b2e9-4400-acf4-1444589b333a (old id 159676)
date added to LUP
2007-06-26 11:09:16
date last changed
2019-02-20 04:17:39
@article{69d69ae1-b2e9-4400-acf4-1444589b333a,
  abstract     = {Arbuscular mycorrhizal (AM) fungi depend on a C supply from the plant host and simultaneously provide phosphorus to the colonized plant. We therefore evaluated the influence of external P on C allocation in monoxenic Daucus carota-Glomus intraradices cultures in an AM symbiosis. Fungal hyphae proliferated from a solid minimal medium containing colonized roots into a C-free liquid minimal medium with high or low P availability. Roots and hyphae were harvested periodically, and the flow of C from roots to fungus was measured by isotope labeling. We also measured induction of a G. intraradices high-affinity P transporter to estimate fungal P demand. The prevailing hypothesis is that high P availability reduces mycorrhizal fungal growth, but we found that C How to the fungus was initially highest at the high P level. Only at later harvests, after 100 days of in vitro. culture, were C flow and fungal growth limited at high P availability. Thus, AM fungi can benefit initially from P-enriched environments in terms of plant C allocation. As expected, the P transporter induction was significantly greater at low P availability and greatest in very young mycelia. We found no direct link between C How to the fungus and the P transporter transcription level, which indicates that a good C supply is not essential for induction of the high-affinity P transporter. We describe a mechanism by which P regulates symbiotic C allocation, and we discuss how this mechanism may have evolved in a competitive environment.},
  author       = {Olsson, Pål Axel and Hansson, Maria and Burleigh, Stephen},
  issn         = {0099-2240},
  language     = {eng},
  number       = {6},
  pages        = {4115--4120},
  publisher    = {American Society for Microbiology},
  series       = {Applied and Environmental Microbiology},
  title        = {Effect of P availability on temporal dynamics of carbon allocation and Glomus intraradices high-affinity P transporter gene induction in arbuscular mycorrhiza},
  url          = {http://dx.doi.org/10.1128/AEM.02154-05},
  volume       = {72},
  year         = {2006},
}