GLP-1 released to the mesenteric lymph duct in mice: Effects of glucose and fat.
(2014) In Regulatory Peptides 189(Feb 28). p.40-45- Abstract
- Using a newly developed in vivo model measuring glucagon-like peptide-1 (GLP-1) in gut lymphatics in mice, we quantified GLP-1 secretion in vivo after glucose versus fat ingestion with and without concomitant DPP-4 inhibition. The mesenteric lymphatic duct was cannulated in anesthetized C57BL6/J mice and lymph was collected in 30min intervals. Glucose or fat emulsion (Intralipid(R)) (0.03, 0.1 or 0.3kcal) with or without DPP-4-inhibition (NVP DPP728; 10μmol/kg) was administered by gastric gavage. Basal intact GLP-1 levels were 0.37±0.04pmol/l (n=61) in lymph compared to 0.07±0.03 in plasma (n=6; P=0.04) and basal DPP-4 activity was 4.7±0.3pmol/min/μl in lymph (n=23) compared to 22.3±0.9pmol/min/μl in plasma (n=8; P<0.001). Lymph flow... (More)
- Using a newly developed in vivo model measuring glucagon-like peptide-1 (GLP-1) in gut lymphatics in mice, we quantified GLP-1 secretion in vivo after glucose versus fat ingestion with and without concomitant DPP-4 inhibition. The mesenteric lymphatic duct was cannulated in anesthetized C57BL6/J mice and lymph was collected in 30min intervals. Glucose or fat emulsion (Intralipid(R)) (0.03, 0.1 or 0.3kcal) with or without DPP-4-inhibition (NVP DPP728; 10μmol/kg) was administered by gastric gavage. Basal intact GLP-1 levels were 0.37±0.04pmol/l (n=61) in lymph compared to 0.07±0.03 in plasma (n=6; P=0.04) and basal DPP-4 activity was 4.7±0.3pmol/min/μl in lymph (n=23) compared to 22.3±0.9pmol/min/μl in plasma (n=8; P<0.001). Lymph flow increased from 1.2±0.1μl/min to 2.3±02μl/min at 30min after glucose and fat administration, with no difference between type of challenge or dose (n=81). Lymph GLP-1 levels increased calorie-dependently after both glucose and fat but with different time courses in that glucose induced a transient increase which had returned to baseline after 90min whereas the lipid induced a sustained increase which was still elevated above baseline after 210min. Lymph GLP-1 appearance during 210min was two to three-fold higher after glucose (7.4±2.3fmol at 0.3kcal) than after isocaloric fat (2.9±0.8fmol at 0.3kcal; P<0.001). The slope between caloric load and lymph GLP-1 appearance was, however, identical after glucose and fat. We conclude that lymph GLP-1 is higher than plasma GLP-1 whereas lymph DPP-4 activity is lower than plasma DPP-4 activity and that both glucose and fat clearly stimulate GLP-1 secretion calorie-dependently in vivo but with different time courses. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/4384339
- author
- Ohlsson, Lena LU ; Kohan, Alison B ; Tso, Patrick and Ahrén, Bo LU
- organization
- publishing date
- 2014
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Regulatory Peptides
- volume
- 189
- issue
- Feb 28
- pages
- 40 - 45
- publisher
- Elsevier
- external identifiers
-
- pmid:24583245
- wos:000336472800007
- scopus:84895731878
- ISSN
- 1873-1686
- DOI
- 10.1016/j.regpep.2014.02.001
- language
- English
- LU publication?
- yes
- id
- 6b63b14f-2a76-425f-9619-02dbbfac8acc (old id 4384339)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/24583245?dopt=Abstract
- date added to LUP
- 2016-04-01 10:59:06
- date last changed
- 2024-01-07 05:57:01
@article{6b63b14f-2a76-425f-9619-02dbbfac8acc, abstract = {{Using a newly developed in vivo model measuring glucagon-like peptide-1 (GLP-1) in gut lymphatics in mice, we quantified GLP-1 secretion in vivo after glucose versus fat ingestion with and without concomitant DPP-4 inhibition. The mesenteric lymphatic duct was cannulated in anesthetized C57BL6/J mice and lymph was collected in 30min intervals. Glucose or fat emulsion (Intralipid(R)) (0.03, 0.1 or 0.3kcal) with or without DPP-4-inhibition (NVP DPP728; 10μmol/kg) was administered by gastric gavage. Basal intact GLP-1 levels were 0.37±0.04pmol/l (n=61) in lymph compared to 0.07±0.03 in plasma (n=6; P=0.04) and basal DPP-4 activity was 4.7±0.3pmol/min/μl in lymph (n=23) compared to 22.3±0.9pmol/min/μl in plasma (n=8; P<0.001). Lymph flow increased from 1.2±0.1μl/min to 2.3±02μl/min at 30min after glucose and fat administration, with no difference between type of challenge or dose (n=81). Lymph GLP-1 levels increased calorie-dependently after both glucose and fat but with different time courses in that glucose induced a transient increase which had returned to baseline after 90min whereas the lipid induced a sustained increase which was still elevated above baseline after 210min. Lymph GLP-1 appearance during 210min was two to three-fold higher after glucose (7.4±2.3fmol at 0.3kcal) than after isocaloric fat (2.9±0.8fmol at 0.3kcal; P<0.001). The slope between caloric load and lymph GLP-1 appearance was, however, identical after glucose and fat. We conclude that lymph GLP-1 is higher than plasma GLP-1 whereas lymph DPP-4 activity is lower than plasma DPP-4 activity and that both glucose and fat clearly stimulate GLP-1 secretion calorie-dependently in vivo but with different time courses.}}, author = {{Ohlsson, Lena and Kohan, Alison B and Tso, Patrick and Ahrén, Bo}}, issn = {{1873-1686}}, language = {{eng}}, number = {{Feb 28}}, pages = {{40--45}}, publisher = {{Elsevier}}, series = {{Regulatory Peptides}}, title = {{GLP-1 released to the mesenteric lymph duct in mice: Effects of glucose and fat.}}, url = {{https://lup.lub.lu.se/search/files/2285414/4645987.pdf}}, doi = {{10.1016/j.regpep.2014.02.001}}, volume = {{189}}, year = {{2014}}, }