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Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.

Kaieda, Shuji LU ; Setlow, Barbara; Setlow, Peter and Halle, Bertil LU (2013) In Biophysical Journal 105(9). p.2016-2023
Abstract
Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor... (More)
Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Biophysical Journal
volume
105
issue
9
pages
2016 - 2023
publisher
Cell Press
external identifiers
  • wos:000326858400011
  • pmid:24209846
  • scopus:84887332460
ISSN
1542-0086
DOI
10.1016/j.bpj.2013.09.022
language
English
LU publication?
yes
id
6bdb4dc2-da82-411c-aa4f-38dff9ea74b8 (old id 4179627)
date added to LUP
2013-12-10 16:43:57
date last changed
2019-06-04 01:21:19
@article{6bdb4dc2-da82-411c-aa4f-38dff9ea74b8,
  abstract     = {Bacterial spores in a metabolically dormant state can survive long periods without nutrients under extreme environmental conditions. The molecular basis of spore dormancy is not well understood, but the distribution and physical state of water within the spore is thought to play an important role. Two scenarios have been proposed for the spore's core region, containing the DNA and most enzymes. In the gel scenario, the core is a structured macromolecular framework permeated by mobile water. In the glass scenario, the entire core, including the water, is an amorphous solid and the quenched molecular diffusion accounts for the spore's dormancy and thermal stability. Here, we use (2)H magnetic relaxation dispersion to selectively monitor water mobility in the core of Bacillus subtilis spores in the presence and absence of core Mn(2+) ions. We also report and analyze the solid-state (2)H NMR spectrum from these spores. Our NMR data clearly support the gel scenario with highly mobile core water (∼25 ps average rotational correlation time). Furthermore, we find that the large depot of manganese in the core is nearly anhydrous, with merely 1.7% on average of the maximum sixfold water coordination.},
  author       = {Kaieda, Shuji and Setlow, Barbara and Setlow, Peter and Halle, Bertil},
  issn         = {1542-0086},
  language     = {eng},
  number       = {9},
  pages        = {2016--2023},
  publisher    = {Cell Press},
  series       = {Biophysical Journal},
  title        = {Mobility of Core Water in Bacillus subtilis Spores by (2)H NMR.},
  url          = {http://dx.doi.org/10.1016/j.bpj.2013.09.022},
  volume       = {105},
  year         = {2013},
}