Biogeographical variation in antimicrobial resistance in rivers is influenced by agriculture and is spread through bacteriophages
Andersson, Tilde LU ; Adell, Aiko D. ; Moreno-Switt, Andrea I. ; Spégel, Peter LU ; Turner, Charlotta LU
; Overballe-Petersen, Søren
; Fuursted, Kurt
and Lood, Rolf
LU
(2022)
In Environmental Microbiology
24(10).
p.4869-4884
- Abstract
Antibiotic resistance is currently an extensive medical challenge worldwide, with global numbers increasing steadily. Recent data have highlighted wastewater treatment plants as a reservoir of resistance genes. The impact of these findings for human health can best be summarized using a One Health concept. However, the molecular mechanisms impacting resistance spread have not been carefully evaluated. Bacterial viruses, that is bacteriophages, have recently been shown to be important mediators of bacterial resistance genes in environmental milieus and are transferrable to human pathogens. Herein, we investigated the biogeographical impact on resistance spread through river-borne bacteriophages using amplicon deep sequencing of the... (More)
Antibiotic resistance is currently an extensive medical challenge worldwide, with global numbers increasing steadily. Recent data have highlighted wastewater treatment plants as a reservoir of resistance genes. The impact of these findings for human health can best be summarized using a One Health concept. However, the molecular mechanisms impacting resistance spread have not been carefully evaluated. Bacterial viruses, that is bacteriophages, have recently been shown to be important mediators of bacterial resistance genes in environmental milieus and are transferrable to human pathogens. Herein, we investigated the biogeographical impact on resistance spread through river-borne bacteriophages using amplicon deep sequencing of the microbiota, absolute quantification of resistance genes using ddPCR, and phage induction capacity within wastewater. Microbial biodiversity of the rivers is significantly affected by river site, surrounding milieu and time of sampling. Furthermore, areas of land associated with agriculture had a significantly higher ability to induce bacteriophages carrying antibiotic resistance genes, indicating their impact on resistance spread. It is imperative that we continue to analyse global antibiotic resistance problem from a One Health perspective to gain novel insights into mechanisms of resistance spread.
(Less)
- author
- Andersson, Tilde
LU
; Adell, Aiko D.
; Moreno-Switt, Andrea I.
; Spégel, Peter
LU
; Turner, Charlotta
LU
; Overballe-Petersen, Søren
; Fuursted, Kurt
and Lood, Rolf
LU
- organization
- publishing date
- 2022-07-07
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Environmental Microbiology
- volume
- 24
- issue
- 10
- pages
- 4869 - 4884
- publisher
- Wiley-Blackwell
- external identifiers
-
- scopus:85133499317
- pmid:35799549
- ISSN
- 1462-2912
- DOI
- 10.1111/1462-2920.16122
- language
- English
- LU publication?
- yes
- id
- 6c61dea8-6e3f-4be2-a845-cad57d6b2bb8
- date added to LUP
- 2022-10-04 09:22:26
- date last changed
- 2024-05-02 14:16:59
@article{6c61dea8-6e3f-4be2-a845-cad57d6b2bb8,
abstract = {{<p>Antibiotic resistance is currently an extensive medical challenge worldwide, with global numbers increasing steadily. Recent data have highlighted wastewater treatment plants as a reservoir of resistance genes. The impact of these findings for human health can best be summarized using a One Health concept. However, the molecular mechanisms impacting resistance spread have not been carefully evaluated. Bacterial viruses, that is bacteriophages, have recently been shown to be important mediators of bacterial resistance genes in environmental milieus and are transferrable to human pathogens. Herein, we investigated the biogeographical impact on resistance spread through river-borne bacteriophages using amplicon deep sequencing of the microbiota, absolute quantification of resistance genes using ddPCR, and phage induction capacity within wastewater. Microbial biodiversity of the rivers is significantly affected by river site, surrounding milieu and time of sampling. Furthermore, areas of land associated with agriculture had a significantly higher ability to induce bacteriophages carrying antibiotic resistance genes, indicating their impact on resistance spread. It is imperative that we continue to analyse global antibiotic resistance problem from a One Health perspective to gain novel insights into mechanisms of resistance spread.</p>}},
author = {{Andersson, Tilde and Adell, Aiko D. and Moreno-Switt, Andrea I. and Spégel, Peter and Turner, Charlotta and Overballe-Petersen, Søren and Fuursted, Kurt and Lood, Rolf}},
issn = {{1462-2912}},
language = {{eng}},
month = {{07}},
number = {{10}},
pages = {{4869--4884}},
publisher = {{Wiley-Blackwell}},
series = {{Environmental Microbiology}},
title = {{Biogeographical variation in antimicrobial resistance in rivers is influenced by agriculture and is spread through bacteriophages}},
url = {{http://dx.doi.org/10.1111/1462-2920.16122}},
doi = {{10.1111/1462-2920.16122}},
volume = {{24}},
year = {{2022}},
}