Silica seed particles improve the efficiency and throughput of nanoparticle acoustic trapping
(2024) In Physical Review Applied 21(3).- Abstract
Silica has rarely been used as a seed particle material in acoustic trapping of nanoparticles. Here we use fluorescent nanoparticles, which are frequently used as a model system, to demonstrate that throughput and nanoparticle trapping efficiency can be improved by using silica seed particles as opposed to traditionally used polystyrene seed particles. The 10 times larger dipole scattering coefficient of silica seed particles compared with polystyrene seed particles in water leads to a higher retention force against fluid flow and thus enables higher throughput. Seed particles retained at an actuation voltage of approximately 10 V p.p. can withstand flow rates up to 2100 ± 200 μl/min for silica and 200 ± 50 μl/min for polystyrene.... (More)
Silica has rarely been used as a seed particle material in acoustic trapping of nanoparticles. Here we use fluorescent nanoparticles, which are frequently used as a model system, to demonstrate that throughput and nanoparticle trapping efficiency can be improved by using silica seed particles as opposed to traditionally used polystyrene seed particles. The 10 times larger dipole scattering coefficient of silica seed particles compared with polystyrene seed particles in water leads to a higher retention force against fluid flow and thus enables higher throughput. Seed particles retained at an actuation voltage of approximately 10 V p.p. can withstand flow rates up to 2100 ± 200 μl/min for silica and 200 ± 50 μl/min for polystyrene. Furthermore, silica is found to be 40%-2000% more efficient (number of trapped nanoparticles as measured by fluorescent intensity) than polystyrene seed particles in trapping 270-nm polystyrene nanoparticles from suspensions of 1010-1011 particles/ml. Moreover, after enriching nanoparticles into a silica seed particle cluster, the washing flow rate can be increased from 30 μl/min to 200 μl/min (the flow rate at which polystyrene clusters are unstable), halving the total sample processing time without losing the silica seed particle cluster or compromising the nanoparticle trapping efficiency. Thus, material properties (particularly density) of the seed particles are critical to both nanoparticle trapping efficiency and throughput.
(Less)
- author
- Havers, Megan LU ; Baasch, Thierry LU ; Lenshof, Andreas LU ; Evander, Mikael LU and Laurell, Thomas LU
- organization
-
- LU Profile Area: Light and Materials
- LTH Profile Area: Nanoscience and Semiconductor Technology
- LTH Profile Area: Engineering Health
- MultiPark: Multidisciplinary research focused on Parkinson´s disease
- NanoLund: Centre for Nanoscience
- Department of Biomedical Engineering
- LUCC: Lund University Cancer Centre
- Acoustofluidics group (research group)
- SEBRA Sepsis and Bacterial Resistance Alliance (research group)
- publishing date
- 2024-03
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Physical Review Applied
- volume
- 21
- issue
- 3
- article number
- 034016
- publisher
- American Physical Society
- external identifiers
-
- scopus:85187540458
- ISSN
- 2331-7019
- DOI
- 10.1103/PhysRevApplied.21.034016
- language
- English
- LU publication?
- yes
- id
- 6cca7d2a-2ff6-48e7-b7ab-6d349135a86c
- date added to LUP
- 2024-04-10 13:40:53
- date last changed
- 2024-04-10 13:42:20
@article{6cca7d2a-2ff6-48e7-b7ab-6d349135a86c, abstract = {{<p>Silica has rarely been used as a seed particle material in acoustic trapping of nanoparticles. Here we use fluorescent nanoparticles, which are frequently used as a model system, to demonstrate that throughput and nanoparticle trapping efficiency can be improved by using silica seed particles as opposed to traditionally used polystyrene seed particles. The 10 times larger dipole scattering coefficient of silica seed particles compared with polystyrene seed particles in water leads to a higher retention force against fluid flow and thus enables higher throughput. Seed particles retained at an actuation voltage of approximately 10 V p.p. can withstand flow rates up to 2100 ± 200 μl/min for silica and 200 ± 50 μl/min for polystyrene. Furthermore, silica is found to be 40%-2000% more efficient (number of trapped nanoparticles as measured by fluorescent intensity) than polystyrene seed particles in trapping 270-nm polystyrene nanoparticles from suspensions of 1010-1011 particles/ml. Moreover, after enriching nanoparticles into a silica seed particle cluster, the washing flow rate can be increased from 30 μl/min to 200 μl/min (the flow rate at which polystyrene clusters are unstable), halving the total sample processing time without losing the silica seed particle cluster or compromising the nanoparticle trapping efficiency. Thus, material properties (particularly density) of the seed particles are critical to both nanoparticle trapping efficiency and throughput.</p>}}, author = {{Havers, Megan and Baasch, Thierry and Lenshof, Andreas and Evander, Mikael and Laurell, Thomas}}, issn = {{2331-7019}}, language = {{eng}}, number = {{3}}, publisher = {{American Physical Society}}, series = {{Physical Review Applied}}, title = {{Silica seed particles improve the efficiency and throughput of nanoparticle acoustic trapping}}, url = {{http://dx.doi.org/10.1103/PhysRevApplied.21.034016}}, doi = {{10.1103/PhysRevApplied.21.034016}}, volume = {{21}}, year = {{2024}}, }