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Adsorption from lipase-surfactant solutions onto methylated silica surfaces

Wannerberger, Kristin; Wahlgren, Marie LU and Arnebrant, Thomas (1996) In Colloids and Surfaces B: Biointerfaces 6(1). p.27-36
Abstract (Swedish)
In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.

From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period.... (More)
In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.

From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period. This was found for all the lipase-surfactant mixtures in the study. However, the amount of lipase adsorbed was larger after rinsing in mixture with SDS, compared with C12E5. The results from addition of surfactant after lipase adsorption indicated that the lipase was replaced by surfactant. This was found for SDS and C12E5 at both the hydrophobic surface and the surface with intermediate hydrophobicity. (Less)
Abstract
In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.

From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period.... (More)
In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.

From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period. This was found for all the lipase-surfactant mixtures in the study. However, the amount of lipase adsorbed was larger after rinsing in mixture with SDS, compared with C12E5. The results from addition of surfactant after lipase adsorption indicated that the lipase was replaced by surfactant. This was found for SDS and C12E5 at both the hydrophobic surface and the surface with intermediate hydrophobicity. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
6
issue
1
pages
10 pages
publisher
Elsevier
external identifiers
  • scopus:0029888067
ISSN
1873-4367
DOI
10.1016/0927-7765(95)01238-9
language
English
LU publication?
yes
id
6ce09746-54ae-462a-b10f-e3b67ff64df0
date added to LUP
2016-04-15 19:34:01
date last changed
2017-08-06 05:06:10
@article{6ce09746-54ae-462a-b10f-e3b67ff64df0,
  abstract     = {In situ ellipsometry was used to study the adsorption/desorption of highly purified lipase from Humicola lanuginosa in mixtures with surfactants, at the solid/liquid interface. The effect of the surfactant was studied both when it was allowed to adsorb in mixture with lipase and when added after lipase adsorption. Silica surfaces, totally or partially methylated, were used and the surfactants were SDS (anionic), C12E5 and a commercial alcohol ethoxylate (AE) (both nonionic). The experiments were carried out so as to simulate a laundry process and the pH throughout the study was kept at 9 by means of Tris-HCl buffer.<br/><br/>From the results it was shown that the lipase did not adsorb until the diluted, that is during the rinsing period. This was found for all the lipase-surfactant mixtures in the study. However, the amount of lipase adsorbed was larger after rinsing in mixture with SDS, compared with C12E5. The results from addition of surfactant after lipase adsorption indicated that the lipase was replaced by surfactant. This was found for SDS and C12E5 at both the hydrophobic surface and the surface with intermediate hydrophobicity.},
  author       = {Wannerberger, Kristin and Wahlgren, Marie and Arnebrant, Thomas},
  issn         = {1873-4367},
  language     = {eng},
  number       = {1},
  pages        = {27--36},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Adsorption from lipase-surfactant solutions onto methylated silica surfaces},
  url          = {http://dx.doi.org/10.1016/0927-7765(95)01238-9},
  volume       = {6},
  year         = {1996},
}