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Removal of Lysozyme from Methylated Silica Surfaces by a Nonionic Surfactant Pentaethyleneglycol Mono n-Dodecyl Ether (C12E5)

Wahlgren, M LU and Arnebrant, T (1996) In Colloids and Surfaces B: Biointerfaces 6(2). p.63-69
Abstract (Swedish)
The removal of lysozyme adsorbed to a hydrophobic surface by a non-ionic surfactant, C12E5, was investigated using in situ ellipsometry. Both the adsorption and removal of protein were studied at different protein and surfactant concentrations. The surfaces used were methylated silica surfaces and the experiments were carried out at pH 5.6 in 0.01 M NaCl solution. The adsorption isotherm of lysozyme did not reach a plateau level within the concentration range investigated and judging from the adsorbed amount at high protein concentrations the protein adsorbs at least in a bilayer. The adsorbed protein was only to a minor extent removed upon rinsing with buffer and addition of surfactant gave partial removal which was dependent on... (More)
The removal of lysozyme adsorbed to a hydrophobic surface by a non-ionic surfactant, C12E5, was investigated using in situ ellipsometry. Both the adsorption and removal of protein were studied at different protein and surfactant concentrations. The surfaces used were methylated silica surfaces and the experiments were carried out at pH 5.6 in 0.01 M NaCl solution. The adsorption isotherm of lysozyme did not reach a plateau level within the concentration range investigated and judging from the adsorbed amount at high protein concentrations the protein adsorbs at least in a bilayer. The adsorbed protein was only to a minor extent removed upon rinsing with buffer and addition of surfactant gave partial removal which was dependent on surfactant concentration and the amount of protein adsorbed. An increase in the surface concentration of protein led to a decrease in the fraction of the adsorbate that was removed due to surfactants. However, the absolute amount removed did not decrease but levelled off at 1.3 mg m−2. The removal of adsorbed lysozyme started at about the same surfactant concentration as that required for the surfactant to adsorb at the interface and the amount removed by surfactant increased with surfactant concentration up to half its cmc. (Less)
Abstract
The removal of lysozyme adsorbed to a hydrophobic surface by a non-ionic surfactant, C12E5, was investigated using in situ ellipsometry. Both the adsorption and removal of protein were studied at different protein and surfactant concentrations. The surfaces used were methylated silica surfaces and the experiments were carried out at pH 5.6 in 0.01 M NaCl solution. The adsorption isotherm of lysozyme did not reach a plateau level within the concentration range investigated and judging from the adsorbed amount at high protein concentrations the protein adsorbs at least in a bilayer. The adsorbed protein was only to a minor extent removed upon rinsing with buffer and addition of surfactant gave partial removal which was dependent on... (More)
The removal of lysozyme adsorbed to a hydrophobic surface by a non-ionic surfactant, C12E5, was investigated using in situ ellipsometry. Both the adsorption and removal of protein were studied at different protein and surfactant concentrations. The surfaces used were methylated silica surfaces and the experiments were carried out at pH 5.6 in 0.01 M NaCl solution. The adsorption isotherm of lysozyme did not reach a plateau level within the concentration range investigated and judging from the adsorbed amount at high protein concentrations the protein adsorbs at least in a bilayer. The adsorbed protein was only to a minor extent removed upon rinsing with buffer and addition of surfactant gave partial removal which was dependent on surfactant concentration and the amount of protein adsorbed. An increase in the surface concentration of protein led to a decrease in the fraction of the adsorbate that was removed due to surfactants. However, the absolute amount removed did not decrease but levelled off at 1.3 mg m−2. The removal of adsorbed lysozyme started at about the same surfactant concentration as that required for the surfactant to adsorb at the interface and the amount removed by surfactant increased with surfactant concentration up to half its cmc. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
in
Colloids and Surfaces B: Biointerfaces
volume
6
issue
2
pages
7 pages
publisher
Elsevier
external identifiers
  • scopus:0029886160
ISSN
0927-7765
DOI
10.1016/0927-7765(95)01245-1
language
English
LU publication?
yes
id
6f4a45a7-1838-4c08-96c4-77d64f937e08
date added to LUP
2016-04-15 19:34:24
date last changed
2017-06-26 18:04:30
@article{6f4a45a7-1838-4c08-96c4-77d64f937e08,
  abstract     = {The removal of lysozyme adsorbed to a hydrophobic surface by a non-ionic surfactant, C12E5, was investigated using in situ ellipsometry. Both the adsorption and removal of protein were studied at different protein and surfactant concentrations. The surfaces used were methylated silica surfaces and the experiments were carried out at pH 5.6 in 0.01 M NaCl solution. The adsorption isotherm of lysozyme did not reach a plateau level within the concentration range investigated and judging from the adsorbed amount at high protein concentrations the protein adsorbs at least in a bilayer. The adsorbed protein was only to a minor extent removed upon rinsing with buffer and addition of surfactant gave partial removal which was dependent on surfactant concentration and the amount of protein adsorbed. An increase in the surface concentration of protein led to a decrease in the fraction of the adsorbate that was removed due to surfactants. However, the absolute amount removed did not decrease but levelled off at 1.3 mg m−2. The removal of adsorbed lysozyme started at about the same surfactant concentration as that required for the surfactant to adsorb at the interface and the amount removed by surfactant increased with surfactant concentration up to half its cmc.},
  author       = {Wahlgren, M and Arnebrant, T},
  issn         = {0927-7765},
  language     = {eng},
  number       = {2},
  pages        = {63--69},
  publisher    = {Elsevier},
  series       = {Colloids and Surfaces B: Biointerfaces},
  title        = {Removal of Lysozyme from Methylated Silica Surfaces by a Nonionic Surfactant Pentaethyleneglycol Mono n-Dodecyl Ether (C12E5)},
  url          = {http://dx.doi.org/10.1016/0927-7765(95)01245-1},
  volume       = {6},
  year         = {1996},
}