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Cytokine regulation of proteoglycan production in fibroblasts : separate and synergistic effects

Tiedemann, K LU ; Malmström, A LU and Westergren-Thorsson, G LU (1997) In Matrix 15(7). p.78-469
Abstract

We have studied the effects of cytokines, separately or in combination, on the production of proteoglycans in confluent cultures of fibroblasts. The cytokines used were the transforming growth factor-beta (TGF-beta), the platelet derived growth factor-AA (PDGF-AA), the platelet derived growth factor-BB (PDGF-BB) and the epidermal growth factor (EGF). Hyaluronan production increased in cells treated with TGF-beta, PDGF-AA and PDGF-BB. Combining pairs of factors did not contribute further to hyaluronan production, whereas the triple combination of EGF, TGF-beta and PDGF-BB induced an additional 1.9-fold increase. Proteoglycan production was only increased by TGF-beta alone. As for hyaluronan, combining pairs of the cytokines had no... (More)

We have studied the effects of cytokines, separately or in combination, on the production of proteoglycans in confluent cultures of fibroblasts. The cytokines used were the transforming growth factor-beta (TGF-beta), the platelet derived growth factor-AA (PDGF-AA), the platelet derived growth factor-BB (PDGF-BB) and the epidermal growth factor (EGF). Hyaluronan production increased in cells treated with TGF-beta, PDGF-AA and PDGF-BB. Combining pairs of factors did not contribute further to hyaluronan production, whereas the triple combination of EGF, TGF-beta and PDGF-BB induced an additional 1.9-fold increase. Proteoglycan production was only increased by TGF-beta alone. As for hyaluronan, combining pairs of the cytokines had no further effect on metabolism, whereas the combination of EGF, TGF-beta and PDGF-BB induced a further 1.6-fold increase in production and secretion. Compared with the control, an extensive increase in proteoglycan production was generated by the combination of EGF, TGF-beta and PDGF-BB, 7-fold for biglycan, approximately 5-fold for versican and hyaluronan and 2.4-4-fold for heparan sulfate proteoglycan and decorin. Compared with TGF-beta alone, this combination increased, in falling order, the production of heparan sulfate proteoglycan, hyaluronan, biglycan, decorin and versican. The mRNA levels for the various proteoglycans did not completely agree with the changes in production, suggesting that changes not only in synthesis but also in rate of degradation generate these variations. The data indicate that cytokines cooperate to produce a proper and physiological response, one needed by the organism during physiological and pathophysiological remodeling.

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author
organization
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Contribution to journal
publication status
published
keywords
Anticoagulants, Cells, Cultured, Cytokines, Drug Synergism, Fibroblasts, Humans, Hyaluronic Acid, Platelet-Derived Growth Factor, Proteoglycans, Proto-Oncogene Proteins c-sis, Transforming Growth Factor beta, Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
in
Matrix
volume
15
issue
7
pages
78 - 469
publisher
Elsevier
external identifiers
  • scopus:0030905154
ISSN
0945-053X
DOI
10.1016/S0945-053X(97)90020-2
language
English
LU publication?
yes
id
72c5c635-d488-45c9-a542-7da05496df2d
date added to LUP
2017-06-27 14:16:57
date last changed
2017-07-02 05:03:54
@article{72c5c635-d488-45c9-a542-7da05496df2d,
  abstract     = {<p>We have studied the effects of cytokines, separately or in combination, on the production of proteoglycans in confluent cultures of fibroblasts. The cytokines used were the transforming growth factor-beta (TGF-beta), the platelet derived growth factor-AA (PDGF-AA), the platelet derived growth factor-BB (PDGF-BB) and the epidermal growth factor (EGF). Hyaluronan production increased in cells treated with TGF-beta, PDGF-AA and PDGF-BB. Combining pairs of factors did not contribute further to hyaluronan production, whereas the triple combination of EGF, TGF-beta and PDGF-BB induced an additional 1.9-fold increase. Proteoglycan production was only increased by TGF-beta alone. As for hyaluronan, combining pairs of the cytokines had no further effect on metabolism, whereas the combination of EGF, TGF-beta and PDGF-BB induced a further 1.6-fold increase in production and secretion. Compared with the control, an extensive increase in proteoglycan production was generated by the combination of EGF, TGF-beta and PDGF-BB, 7-fold for biglycan, approximately 5-fold for versican and hyaluronan and 2.4-4-fold for heparan sulfate proteoglycan and decorin. Compared with TGF-beta alone, this combination increased, in falling order, the production of heparan sulfate proteoglycan, hyaluronan, biglycan, decorin and versican. The mRNA levels for the various proteoglycans did not completely agree with the changes in production, suggesting that changes not only in synthesis but also in rate of degradation generate these variations. The data indicate that cytokines cooperate to produce a proper and physiological response, one needed by the organism during physiological and pathophysiological remodeling.</p>},
  author       = {Tiedemann, K and Malmström, A and Westergren-Thorsson, G},
  issn         = {0945-053X},
  keyword      = {Anticoagulants,Cells, Cultured,Cytokines,Drug Synergism,Fibroblasts,Humans,Hyaluronic Acid,Platelet-Derived Growth Factor,Proteoglycans,Proto-Oncogene Proteins c-sis,Transforming Growth Factor beta,Comparative Study,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  number       = {7},
  pages        = {78--469},
  publisher    = {Elsevier},
  series       = {Matrix},
  title        = {Cytokine regulation of proteoglycan production in fibroblasts : separate and synergistic effects},
  url          = {http://dx.doi.org/10.1016/S0945-053X(97)90020-2},
  volume       = {15},
  year         = {1997},
}