Understanding the characteristic behaviour of the wild-type and mutant structure of FLT3 protein by computational methods
Younus, Saleena LU ; Tatli, Özge LU ; Nasimian, Ahmad LU ; Kazi, Julhash U. LU
and Rönnstrand, Lars
LU
(2025)
In Computational and Structural Biotechnology Journal
27.
p.4526-4542
- Abstract
FLT3 is a critical prognostic marker in acute myeloid leukemia (AML) due to its high frequency of mutation. Primary mutations in FLT3 include point mutations or deletions in the tyrosine kinase domain (TKD) and internal tandem duplications (ITD) in the juxtamembrane (JM) region. These mutations lead to ligand-independent activation, disrupting the JM region and kinase domain interaction, resulting in autophosphorylation of FLT3 and activation of signalling pathways such as STAT5, MAPK, and AKT, which promotes increased proliferation and resistance to apoptosis. So developing effective therapeutics against these mutant FLT3 proteins is crucial for AML treatment. Here we employed computational approaches, including Molecular Dynamics (MD)... (More)
FLT3 is a critical prognostic marker in acute myeloid leukemia (AML) due to its high frequency of mutation. Primary mutations in FLT3 include point mutations or deletions in the tyrosine kinase domain (TKD) and internal tandem duplications (ITD) in the juxtamembrane (JM) region. These mutations lead to ligand-independent activation, disrupting the JM region and kinase domain interaction, resulting in autophosphorylation of FLT3 and activation of signalling pathways such as STAT5, MAPK, and AKT, which promotes increased proliferation and resistance to apoptosis. So developing effective therapeutics against these mutant FLT3 proteins is crucial for AML treatment. Here we employed computational approaches, including Molecular Dynamics (MD) simulation, cluster analysis, and Normal Mode Analysis, to investigate the dynamic behaviours of FLT3 mutant structures (TKD mutants Y842C/F in FLT3-WT, FLT3- ITD, and ITD mutant in FLT3-WT). The molecular dynamics (MD) simulation studies revealed that all these mutations significantly affect the optimized state, flexibility, and compactness of the FLT3 protein. Cluster analysis also confirmed that these mutations significantly impacted protein flexibility. Specifically, the ITD mutation in the JM region increased the overall flexibility of the FLT3 structure, whereas the point mutations in the TKD domain (TKD; Y842C and Y842F) and the combined mutations of ITD and TKD in the FLT3 protein (ITD + Y842C/F) resulted in more rigid conformations. These findings highlight the distinct conformational effects of FLT3 mutations that contribute to kinase deregulation and underscore their potential as therapeutic targets in AML.
(Less)
- author
- Younus, Saleena
LU
; Tatli, Özge
LU
; Nasimian, Ahmad
LU
; Kazi, Julhash U.
LU
and Rönnstrand, Lars
LU
- organization
- publishing date
- 2025-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- AML, Combination mutation, Flexibility, FLT3 protein, Leukemia, Point mutation, Protein dynamics, RTK's, Structural conformations
- in
- Computational and Structural Biotechnology Journal
- volume
- 27
- pages
- 4526 - 4542
- publisher
- Research Network of Computational and Structural Biotechnology
- external identifiers
-
- pmid:41215958
- scopus:105019933293
- ISSN
- 2001-0370
- DOI
- 10.1016/j.csbj.2025.10.023
- language
- English
- LU publication?
- yes
- additional info
- Publisher Copyright: © 2025 The Authors
- id
- 73238c99-920e-46a6-8601-b6f27bbe72ce
- date added to LUP
- 2025-11-16 16:33:54
- date last changed
- 2025-11-18 03:20:27
@article{73238c99-920e-46a6-8601-b6f27bbe72ce,
abstract = {{<p>FLT3 is a critical prognostic marker in acute myeloid leukemia (AML) due to its high frequency of mutation. Primary mutations in FLT3 include point mutations or deletions in the tyrosine kinase domain (TKD) and internal tandem duplications (ITD) in the juxtamembrane (JM) region. These mutations lead to ligand-independent activation, disrupting the JM region and kinase domain interaction, resulting in autophosphorylation of FLT3 and activation of signalling pathways such as STAT5, MAPK, and AKT, which promotes increased proliferation and resistance to apoptosis. So developing effective therapeutics against these mutant FLT3 proteins is crucial for AML treatment. Here we employed computational approaches, including Molecular Dynamics (MD) simulation, cluster analysis, and Normal Mode Analysis, to investigate the dynamic behaviours of FLT3 mutant structures (TKD mutants Y842C/F in FLT3-WT, FLT3- ITD, and ITD mutant in FLT3-WT). The molecular dynamics (MD) simulation studies revealed that all these mutations significantly affect the optimized state, flexibility, and compactness of the FLT3 protein. Cluster analysis also confirmed that these mutations significantly impacted protein flexibility. Specifically, the ITD mutation in the JM region increased the overall flexibility of the FLT3 structure, whereas the point mutations in the TKD domain (TKD; Y842C and Y842F) and the combined mutations of ITD and TKD in the FLT3 protein (ITD + Y842C/F) resulted in more rigid conformations. These findings highlight the distinct conformational effects of FLT3 mutations that contribute to kinase deregulation and underscore their potential as therapeutic targets in AML.</p>}},
author = {{Younus, Saleena and Tatli, Özge and Nasimian, Ahmad and Kazi, Julhash U. and Rönnstrand, Lars}},
issn = {{2001-0370}},
keywords = {{AML; Combination mutation; Flexibility; FLT3 protein; Leukemia; Point mutation; Protein dynamics; RTK's; Structural conformations}},
language = {{eng}},
pages = {{4526--4542}},
publisher = {{Research Network of Computational and Structural Biotechnology}},
series = {{Computational and Structural Biotechnology Journal}},
title = {{Understanding the characteristic behaviour of the wild-type and mutant structure of FLT3 protein by computational methods}},
url = {{http://dx.doi.org/10.1016/j.csbj.2025.10.023}},
doi = {{10.1016/j.csbj.2025.10.023}},
volume = {{27}},
year = {{2025}},
}