4-quinolone antibiotics : Positive genotoxic screening tests despite an apparent lack of mutation induction
Bredberg, Anders LU ; Brant, Marta LU ; Riesbeck, Kristian LU
; Azou, Yannick
and Forsgren, Arne
LU
(1989)
In Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
211(1).
p.171-180
- Abstract
The effects of different 4-quinolone antibiotic derivatives (4-Qs) in a number of short-term tests commonly employed for the evaluation of genetic toxicity were studied. Incorporation of [3H]thymidine into mitogen-stimulated peripheral blood lymphocytes was strongly enhanced at a low concentration (1.56 μg/ml) for most of the tested 4-Qs, whereas DNA strand breakage in lymphoblastoid cells was evident only for ciprofloxacin (10 μg/ml and upwards), ofloxacin (80 μg/ml) and norfloxacin (160 μg/ml). Ciphrofloxacin induced a significant amount of unscheduled DNA synthesis, but was found to be negative in a shuttle vector plasmid mutation test. Ciprofloxacin (80 μg/ml) did not inhibit enzymes involved in the early steps of... (More)
The effects of different 4-quinolone antibiotic derivatives (4-Qs) in a number of short-term tests commonly employed for the evaluation of genetic toxicity were studied. Incorporation of [3H]thymidine into mitogen-stimulated peripheral blood lymphocytes was strongly enhanced at a low concentration (1.56 μg/ml) for most of the tested 4-Qs, whereas DNA strand breakage in lymphoblastoid cells was evident only for ciprofloxacin (10 μg/ml and upwards), ofloxacin (80 μg/ml) and norfloxacin (160 μg/ml). Ciphrofloxacin induced a significant amount of unscheduled DNA synthesis, but was found to be negative in a shuttle vector plasmid mutation test. Ciprofloxacin (80 μg/ml) did not inhibit enzymes involved in the early steps of pyrimidine biosynthesis. Cell growth was slightly depressed at a concentration of 20 μg/ml, becoming marked at 80 μg/ml. In conculsion, this study seeks to contribute to an improved evaluation of genotoxic screening test data, by focuding attention on the conflicting effects imposed by the 4-Qs on a battery of such tests.
(Less)
- author
- Bredberg, Anders
LU
; Brant, Marta
LU
; Riesbeck, Kristian
LU
; Azou, Yannick
and Forsgren, Arne
LU
- organization
- publishing date
- 1989-01-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- 4-Quinolones, DNA repair, DNA synthesis, Gene mutation, Genetic toxicity
- in
- Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
- volume
- 211
- issue
- 1
- pages
- 171 - 180
- publisher
- Elsevier
- external identifiers
-
- pmid:2921999
- scopus:0024586601
- ISSN
- 1879-2871
- DOI
- 10.1016/0027-5107(89)90117-6
- language
- English
- LU publication?
- yes
- id
- 7376ff6b-f3dd-4a80-8cf6-d7c0c956f196
- date added to LUP
- 2019-03-27 14:24:07
- date last changed
- 2024-01-01 00:13:48
@article{7376ff6b-f3dd-4a80-8cf6-d7c0c956f196,
abstract = {{<p>The effects of different 4-quinolone antibiotic derivatives (4-Qs) in a number of short-term tests commonly employed for the evaluation of genetic toxicity were studied. Incorporation of [<sup>3</sup>H]thymidine into mitogen-stimulated peripheral blood lymphocytes was strongly enhanced at a low concentration (1.56 μg/ml) for most of the tested 4-Qs, whereas DNA strand breakage in lymphoblastoid cells was evident only for ciprofloxacin (10 μg/ml and upwards), ofloxacin (80 μg/ml) and norfloxacin (160 μg/ml). Ciphrofloxacin induced a significant amount of unscheduled DNA synthesis, but was found to be negative in a shuttle vector plasmid mutation test. Ciprofloxacin (80 μg/ml) did not inhibit enzymes involved in the early steps of pyrimidine biosynthesis. Cell growth was slightly depressed at a concentration of 20 μg/ml, becoming marked at 80 μg/ml. In conculsion, this study seeks to contribute to an improved evaluation of genotoxic screening test data, by focuding attention on the conflicting effects imposed by the 4-Qs on a battery of such tests.</p>}},
author = {{Bredberg, Anders and Brant, Marta and Riesbeck, Kristian and Azou, Yannick and Forsgren, Arne}},
issn = {{1879-2871}},
keywords = {{4-Quinolones; DNA repair; DNA synthesis; Gene mutation; Genetic toxicity}},
language = {{eng}},
month = {{01}},
number = {{1}},
pages = {{171--180}},
publisher = {{Elsevier}},
series = {{Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis}},
title = {{4-quinolone antibiotics : Positive genotoxic screening tests despite an apparent lack of mutation induction}},
url = {{http://dx.doi.org/10.1016/0027-5107(89)90117-6}},
doi = {{10.1016/0027-5107(89)90117-6}},
volume = {{211}},
year = {{1989}},
}