Two-enzyme system for the synthesis of 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate
(2000) In Chemistry and Physics of Lipids 104(2). p.175-184- Abstract
A combination of two enzymes, phospholipase D (PL D) and C (PL C), was investigated for the production of two lysophospholipids, 1-lauroyl-rac- glycerophosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The high transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1- lauroyl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1- MDHA), respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two-phase system was used for hydrolysis of the complex phospholipids... (More)
A combination of two enzymes, phospholipase D (PL D) and C (PL C), was investigated for the production of two lysophospholipids, 1-lauroyl-rac- glycerophosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The high transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1- lauroyl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1- MDHA), respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two-phase system was used for hydrolysis of the complex phospholipids by phospholipase C form Bacillus cereus, which released both lysophospholipids. Only trace amounts of phosphatidic acid (PA) were detected showing that the enzyme is highly selective for the release of the diacylglycerol and 1- lauroyl-rac-glycerophosphate and 1-lauroyl-dihydroxyacetonephosphate.
(Less)
- author
- Virto, Carmen LU and Adlercreutz, Patrick LU
- organization
- publishing date
- 2000-02-01
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Acyl- dihydroxyacetonephosphate, Acyl- phosphatidyldihydroxyacetone, Acyl-phosphatidylglycerol, Acyl-rac-glycerophosphate, Phospholipase C, Phospholipase D
- in
- Chemistry and Physics of Lipids
- volume
- 104
- issue
- 2
- pages
- 10 pages
- publisher
- Elsevier
- external identifiers
-
- scopus:0033987313
- pmid:10669309
- ISSN
- 0009-3084
- DOI
- 10.1016/S0009-3084(99)00123-1
- language
- English
- LU publication?
- yes
- id
- 73bf4a3d-a24e-41e0-b4a1-a7b845d30ff4
- date added to LUP
- 2019-06-20 15:57:56
- date last changed
- 2024-10-02 05:55:03
@article{73bf4a3d-a24e-41e0-b4a1-a7b845d30ff4, abstract = {{<p>A combination of two enzymes, phospholipase D (PL D) and C (PL C), was investigated for the production of two lysophospholipids, 1-lauroyl-rac- glycerophosphate (1-LGP) and 1-lauroyl-dihydroxyacetonephosphate (1-LDHAP). The high transphosphatidylation ability of phospholipase D from Streptomyces sp. allowed the formation of 1-lauroyl-phosphatidylglycerol (1-LPG) and 1- lauroyl-phosphatidyldihydroxyacetone (1-LPDHA) from phosphatidylcholine (PC) and 1-monolauroyl-rac-glycerol (1-MLG) and 1-lauroyl-dihydroxyacetone (1- MDHA), respectively. A two-phase system, diethyl ether/water, was chosen for the convenience of the recovery of the water insoluble products. A similar two-phase system was used for hydrolysis of the complex phospholipids by phospholipase C form Bacillus cereus, which released both lysophospholipids. Only trace amounts of phosphatidic acid (PA) were detected showing that the enzyme is highly selective for the release of the diacylglycerol and 1- lauroyl-rac-glycerophosphate and 1-lauroyl-dihydroxyacetonephosphate.</p>}}, author = {{Virto, Carmen and Adlercreutz, Patrick}}, issn = {{0009-3084}}, keywords = {{Acyl- dihydroxyacetonephosphate; Acyl- phosphatidyldihydroxyacetone; Acyl-phosphatidylglycerol; Acyl-rac-glycerophosphate; Phospholipase C; Phospholipase D}}, language = {{eng}}, month = {{02}}, number = {{2}}, pages = {{175--184}}, publisher = {{Elsevier}}, series = {{Chemistry and Physics of Lipids}}, title = {{Two-enzyme system for the synthesis of 1-lauroyl-rac-glycerophosphate (lysophosphatidic acid) and 1-lauroyl-dihydroxyacetonephosphate}}, url = {{http://dx.doi.org/10.1016/S0009-3084(99)00123-1}}, doi = {{10.1016/S0009-3084(99)00123-1}}, volume = {{104}}, year = {{2000}}, }