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Sequential intracerebral transplantation of allogeneic and syngeneic fetal dopamine-rich neuronal tissue in adult rats : will the first graft be rejected?

Widner, H LU and Brundin, P LU (1993) In Cell Transplantation 2(4). p.17-307
Abstract

The immune response against intracerebral grafts of allogeneic fetal dopamine-rich tissue was assessed in adult rats. Sprague-Dawley rats, now outbred, but originating from an inbred stock, were given unilateral 6-hydroxy-dopamine lesions of the mesostriatal pathway, and grafted intrastriatally with mechanically dissociated ventral mesencephalic tissue (embryonic day 13-15) obtained from an inbred Lewis strain. Graft survival was assessed by functional recovery of amphetamine-induced rotational behavior on four different occasions postsurgery, and histologically using catecholamine histofluorescence and tyrosine hydroxylase immunohistochemistry. The following groups were analysed: long-term survival of a single allogeneic graft;... (More)

The immune response against intracerebral grafts of allogeneic fetal dopamine-rich tissue was assessed in adult rats. Sprague-Dawley rats, now outbred, but originating from an inbred stock, were given unilateral 6-hydroxy-dopamine lesions of the mesostriatal pathway, and grafted intrastriatally with mechanically dissociated ventral mesencephalic tissue (embryonic day 13-15) obtained from an inbred Lewis strain. Graft survival was assessed by functional recovery of amphetamine-induced rotational behavior on four different occasions postsurgery, and histologically using catecholamine histofluorescence and tyrosine hydroxylase immunohistochemistry. The following groups were analysed: long-term survival of a single allogeneic graft; survival of a first allogeneic graft with a syngeneic second graft; survival of a first allograft combined with a second allogeneic graft; the survival of bilateral allogeneic grafts following a subsequent orthotopic allogeneic skin graft. Evidence for recipient immunization was obtained using an indirect fluorescent antibody detection technique, Simonsen's Spleen Index (S I) test. Viable grafts, giving rise to behavioral compensation, were present after 40 wk in rats from all groups. The "first" allograft always displayed good survival and function, even following a second intracerebral allograft. However, five of nine "second" allogeneic intracerebral grafts survived poorly. In contrast, all secondary syngeneic grafts survived well. Following the application of a subsequent orthotopic allogeneic skin graft in a subgroup of rats, there was a significantly lower survival of grafted dopamine neurons in the "first" graft.

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published
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keywords
Animals, Brain Tissue Transplantation, Corpus Striatum, Dopamine, Female, Fetal Tissue Transplantation, Graft Rejection, Graft Survival, Immunosuppression, Mesencephalon, Neuroimmunomodulation, Oxidopamine, Pregnancy, Rats, Rats, Inbred Lew, Rats, Sprague-Dawley, Reoperation, Skin Transplantation, Transplantation, Homologous, Transplantation, Isogeneic, Journal Article, Research Support, Non-U.S. Gov't
in
Cell Transplantation
volume
2
issue
4
pages
11 pages
publisher
Cognizant Communication Corporation
external identifiers
  • scopus:0027273274
ISSN
0963-6897
language
English
LU publication?
yes
id
73c21fa2-a86a-4a4a-aab9-e17c367513a9
date added to LUP
2017-04-19 18:29:18
date last changed
2017-05-05 14:27:23
@article{73c21fa2-a86a-4a4a-aab9-e17c367513a9,
  abstract     = {<p>The immune response against intracerebral grafts of allogeneic fetal dopamine-rich tissue was assessed in adult rats. Sprague-Dawley rats, now outbred, but originating from an inbred stock, were given unilateral 6-hydroxy-dopamine lesions of the mesostriatal pathway, and grafted intrastriatally with mechanically dissociated ventral mesencephalic tissue (embryonic day 13-15) obtained from an inbred Lewis strain. Graft survival was assessed by functional recovery of amphetamine-induced rotational behavior on four different occasions postsurgery, and histologically using catecholamine histofluorescence and tyrosine hydroxylase immunohistochemistry. The following groups were analysed: long-term survival of a single allogeneic graft; survival of a first allogeneic graft with a syngeneic second graft; survival of a first allograft combined with a second allogeneic graft; the survival of bilateral allogeneic grafts following a subsequent orthotopic allogeneic skin graft. Evidence for recipient immunization was obtained using an indirect fluorescent antibody detection technique, Simonsen's Spleen Index (S I) test. Viable grafts, giving rise to behavioral compensation, were present after 40 wk in rats from all groups. The "first" allograft always displayed good survival and function, even following a second intracerebral allograft. However, five of nine "second" allogeneic intracerebral grafts survived poorly. In contrast, all secondary syngeneic grafts survived well. Following the application of a subsequent orthotopic allogeneic skin graft in a subgroup of rats, there was a significantly lower survival of grafted dopamine neurons in the "first" graft.</p>},
  author       = {Widner, H and Brundin, P},
  issn         = {0963-6897},
  keyword      = {Animals,Brain Tissue Transplantation,Corpus Striatum,Dopamine,Female,Fetal Tissue Transplantation,Graft Rejection,Graft Survival,Immunosuppression,Mesencephalon,Neuroimmunomodulation,Oxidopamine,Pregnancy,Rats,Rats, Inbred Lew,Rats, Sprague-Dawley,Reoperation,Skin Transplantation,Transplantation, Homologous,Transplantation, Isogeneic,Journal Article,Research Support, Non-U.S. Gov't},
  language     = {eng},
  month        = {07},
  number       = {4},
  pages        = {17--307},
  publisher    = {Cognizant Communication Corporation},
  series       = {Cell Transplantation},
  title        = {Sequential intracerebral transplantation of allogeneic and syngeneic fetal dopamine-rich neuronal tissue in adult rats : will the first graft be rejected?},
  volume       = {2},
  year         = {1993},
}