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The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A2 and triacylglycerol lipase

Wadsäter, Maria LU ; Barauskas, Justas LU ; Tiberg, Fredrik LU and Nylander, Tommy LU (2017) In Chemistry and Physics of Lipids
Abstract

The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A2 (PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of... (More)

The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A2 (PLA2) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA2, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.

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organization
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type
Contribution to journal
publication status
epub
subject
keywords
Cryogenic transmission electron microscopy, Lipid liquid crystalline nano-particles, Lipolytic enzymes, Phospholipase A, Small angle X-ray diffraction, Triacylglycerol lipase
in
Chemistry and Physics of Lipids
publisher
Elsevier
external identifiers
  • scopus:85034242000
ISSN
0009-3084
DOI
10.1016/j.chemphyslip.2017.11.011
language
English
LU publication?
yes
id
742df5e9-4f51-4a02-a6d8-5a18cb349d1a
date added to LUP
2017-12-11 07:50:05
date last changed
2018-01-07 12:28:00
@article{742df5e9-4f51-4a02-a6d8-5a18cb349d1a,
  abstract     = {<p>The effects of different lipolytic enzymes on the structure of lipid liquid crystalline nano-particles (LCNP) have been investigated by cryogenic transmission electron microscopy (cryo-TEM) and synchrotron small angle X-ray diffraction (SAXD). Here we used highly structured cubic micellar (Fd3m) nanoparticles of 50/50 (wt%/wt%) soy phosphatidyl choline (SPC)/glycerol dioleate (GDO) as substrate. Two types of lipolytic enzymes were used, phospholipase A<sub>2</sub> (PLA<sub>2</sub>) that catalyses degradation of the phospholipid component, SPC, and porcine pancreatic triacylglycerol lipase (TGL) that facilitate the hydrolysis of the diglyceride, GDO. Evolution of the structure was found to be very different and linked to specificity of the two types of enzymes. PLA<sub>2</sub>, which hydrolyses the lamellar forming component, SPC, induces a reversed micellar lipid phase, while TGL which hydrolysis the reverse phase forming compound, GDO, induces a lamellar phase.</p>},
  author       = {Wadsäter, Maria and Barauskas, Justas and Tiberg, Fredrik and Nylander, Tommy},
  issn         = {0009-3084},
  keyword      = {Cryogenic transmission electron microscopy,Lipid liquid crystalline nano-particles,Lipolytic enzymes,Phospholipase A,Small angle X-ray diffraction,Triacylglycerol lipase},
  language     = {eng},
  month        = {11},
  publisher    = {Elsevier},
  series       = {Chemistry and Physics of Lipids},
  title        = {The lipolytic degradation of highly structured cubic micellar nanoparticles of soy phosphatidylcholine and glycerol dioleate by phospholipase A<sub>2</sub> and triacylglycerol lipase},
  url          = {http://dx.doi.org/10.1016/j.chemphyslip.2017.11.011},
  year         = {2017},
}