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Validation of an air–liquid interface toxicological set-up using Cu, Pd, and Ag well-characterized nanostructured aggregates and spheres

Svensson, Christian LU ; Ameer, Shegufta LU ; Ludvigsson, Linus LU ; Ali, Neserin LU orcid ; Alhamdow, Ayman LU ; Messing, Maria LU ; Pagels, Joakim LU ; Gudmundsson, Anders LU ; Bohgard, Mats LU and Sanfins, E. , et al. (2016) In Journal of Nanoparticle Research 18(4).
Abstract

Abstract: Systems for studying the toxicity of metal aggregates on the airways are normally not suited for evaluating the effects of individual particle characteristics. This study validates a set-up for toxicological studies of metal aggregates using an air–liquid interface approach. The set-up used a spark discharge generator capable of generating aerosol metal aggregate particles and sintered near spheres. The set-up also contained an exposure chamber, The Nano Aerosol Chamber for In Vitro Toxicity (NACIVT). The system facilitates online characterization capabilities of mass mobility, mass concentration, and number size distribution to determine the exposure. By dilution, the desired exposure level was controlled. Primary and... (More)

Abstract: Systems for studying the toxicity of metal aggregates on the airways are normally not suited for evaluating the effects of individual particle characteristics. This study validates a set-up for toxicological studies of metal aggregates using an air–liquid interface approach. The set-up used a spark discharge generator capable of generating aerosol metal aggregate particles and sintered near spheres. The set-up also contained an exposure chamber, The Nano Aerosol Chamber for In Vitro Toxicity (NACIVT). The system facilitates online characterization capabilities of mass mobility, mass concentration, and number size distribution to determine the exposure. By dilution, the desired exposure level was controlled. Primary and cancerous airway cells were exposed to copper (Cu), palladium (Pd), and silver (Ag) aggregates, 50–150 nm in median diameter. The aggregates were composed of primary particles 2, respectively, were achieved. Viability was measured by WST-1 assay, cytokines (Il-6, Il-8, TNF-a, MCP) by Luminex technology. Statistically significant effects and dose response on cytokine expression were observed for SAEC cells after exposure to Cu, Pd, or Ag particles. Also, a positive dose response was observed for SAEC viability after Cu exposure. For A549 cells, statistically significant effects on viability were observed after exposure to Cu and Pd particles. The set-up produced a stable flow of aerosol particles with an exposure and dose expressed in terms of number, mass, and surface area. Exposure-related effects on the airway cellular models could be asserted. Graphical Abstract: [Figure not available: see fulltext.]

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organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
A549, Aggregates, Air–liquid interface, Cytokines, DMA-APM, Health effects, NACIVT, SAEC, Toxicity
in
Journal of Nanoparticle Research
volume
18
issue
4
article number
86
publisher
Springer
external identifiers
  • pmid:27069401
  • wos:000373156500004
  • scopus:84961659569
ISSN
1388-0764
DOI
10.1007/s11051-016-3389-y
language
English
LU publication?
yes
id
745a8380-23a0-4d99-aa7d-a2000e12334d
date added to LUP
2016-06-16 11:59:28
date last changed
2025-01-12 07:21:06
@article{745a8380-23a0-4d99-aa7d-a2000e12334d,
  abstract     = {{<p>Abstract: Systems for studying the toxicity of metal aggregates on the airways are normally not suited for evaluating the effects of individual particle characteristics. This study validates a set-up for toxicological studies of metal aggregates using an air–liquid interface approach. The set-up used a spark discharge generator capable of generating aerosol metal aggregate particles and sintered near spheres. The set-up also contained an exposure chamber, The Nano Aerosol Chamber for In Vitro Toxicity (NACIVT). The system facilitates online characterization capabilities of mass mobility, mass concentration, and number size distribution to determine the exposure. By dilution, the desired exposure level was controlled. Primary and cancerous airway cells were exposed to copper (Cu), palladium (Pd), and silver (Ag) aggregates, 50–150 nm in median diameter. The aggregates were composed of primary particles 2, respectively, were achieved. Viability was measured by WST-1 assay, cytokines (Il-6, Il-8, TNF-a, MCP) by Luminex technology. Statistically significant effects and dose response on cytokine expression were observed for SAEC cells after exposure to Cu, Pd, or Ag particles. Also, a positive dose response was observed for SAEC viability after Cu exposure. For A549 cells, statistically significant effects on viability were observed after exposure to Cu and Pd particles. The set-up produced a stable flow of aerosol particles with an exposure and dose expressed in terms of number, mass, and surface area. Exposure-related effects on the airway cellular models could be asserted. Graphical Abstract: [Figure not available: see fulltext.]</p>}},
  author       = {{Svensson, Christian and Ameer, Shegufta and Ludvigsson, Linus and Ali, Neserin and Alhamdow, Ayman and Messing, Maria and Pagels, Joakim and Gudmundsson, Anders and Bohgard, Mats and Sanfins, E. and Kåredal, Monica and Broberg Palmgren, Karin and Rissler, Jenny}},
  issn         = {{1388-0764}},
  keywords     = {{A549; Aggregates; Air–liquid interface; Cytokines; DMA-APM; Health effects; NACIVT; SAEC; Toxicity}},
  language     = {{eng}},
  month        = {{04}},
  number       = {{4}},
  publisher    = {{Springer}},
  series       = {{Journal of Nanoparticle Research}},
  title        = {{Validation of an air–liquid interface toxicological set-up using Cu, Pd, and Ag well-characterized nanostructured aggregates and spheres}},
  url          = {{http://dx.doi.org/10.1007/s11051-016-3389-y}},
  doi          = {{10.1007/s11051-016-3389-y}},
  volume       = {{18}},
  year         = {{2016}},
}