Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.
(2015) In Scientific Reports 5.- Abstract
- Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and... (More)
- Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS. (Less)
Please use this url to cite or link to this publication:
https://lup.lub.lu.se/record/7485604
- author
- Gu Urban, Gucci Jijuan
; Friedman, Mikaela
; Ren, Ping
; Törn, Carina
LU
; Fex, Malin
LU
; Hampe, Christiane S
; Lernmark, Åke
LU
; Landegren, Ulf LU and Kamali-Moghaddam, Masood
- organization
- publishing date
- 2015
- type
- Contribution to journal
- publication status
- published
- subject
- in
- Scientific Reports
- volume
- 5
- article number
- 11196
- publisher
- Nature Publishing Group
- external identifiers
-
- pmid:26080009
- wos:000356508700001
- scopus:84935867444
- pmid:26080009
- ISSN
- 2045-2322
- DOI
- 10.1038/srep11196
- language
- English
- LU publication?
- yes
- id
- 0c1f968f-3ca3-40b6-a59b-6c88bc4e37cf (old id 7485604)
- alternative location
- http://www.ncbi.nlm.nih.gov/pubmed/26080009?dopt=Abstract
- date added to LUP
- 2016-04-01 13:32:35
- date last changed
- 2024-05-08 11:14:22
@article{0c1f968f-3ca3-40b6-a59b-6c88bc4e37cf, abstract = {{Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.}}, author = {{Gu Urban, Gucci Jijuan and Friedman, Mikaela and Ren, Ping and Törn, Carina and Fex, Malin and Hampe, Christiane S and Lernmark, Åke and Landegren, Ulf and Kamali-Moghaddam, Masood}}, issn = {{2045-2322}}, language = {{eng}}, publisher = {{Nature Publishing Group}}, series = {{Scientific Reports}}, title = {{Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.}}, url = {{https://lup.lub.lu.se/search/files/3438864/8776915}}, doi = {{10.1038/srep11196}}, volume = {{5}}, year = {{2015}}, }