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Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.

Gu Urban, Gucci Jijuan; Friedman, Mikaela; Ren, Ping; Törn, Carina LU ; Fex, Malin LU ; Hampe, Christiane S; Lernmark, Åke LU ; Landegren, Ulf LU and Kamali-Moghaddam, Masood (2015) In Scientific Reports 5.
Abstract
Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and... (More)
Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS. (Less)
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author
organization
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type
Contribution to journal
publication status
published
subject
in
Scientific Reports
volume
5
publisher
Nature Publishing Group
external identifiers
  • pmid:26080009
  • wos:000356508700001
  • scopus:84935867444
ISSN
2045-2322
DOI
10.1038/srep11196
language
English
LU publication?
yes
id
0c1f968f-3ca3-40b6-a59b-6c88bc4e37cf (old id 7485604)
alternative location
http://www.ncbi.nlm.nih.gov/pubmed/26080009?dopt=Abstract
date added to LUP
2015-07-08 18:17:59
date last changed
2017-01-01 05:43:49
@article{0c1f968f-3ca3-40b6-a59b-6c88bc4e37cf,
  abstract     = {Glutamic acid decarboxylase 65 (GAD65) and autoantibodies specific for GAD65 (GADA) are associated with autoimmune diseases including Stiff Person Syndrome (SPS) and Type 1 diabetes (T1D). GADA is recognized as a biomarker of value for clinical diagnosis and prognostication in these diseases. Nonetheless, it remains medically interesting to develop sensitive and specific assays to detect GAD65 preceding GADA emergence, and to monitor GADA-GAD65 immune complexes in blood samples. In the present study, we developed a highly sensitive proximity ligation assay to measure serum GAD65. This novel assay allowed detection of as little as 0.65 pg/ml GAD65. We were also able to detect immune complexes involving GAD65 and GADA. Both free GAD65 and GAD65-GADA levels were significantly higher in serum samples from SPS patients compared to healthy controls. The proximity ligation assays applied for detection of GAD65 and its immune complexes may thus enable improved diagnosis and better understanding of SPS.},
  articleno    = {11196},
  author       = {Gu Urban, Gucci Jijuan and Friedman, Mikaela and Ren, Ping and Törn, Carina and Fex, Malin and Hampe, Christiane S and Lernmark, Åke and Landegren, Ulf and Kamali-Moghaddam, Masood},
  issn         = {2045-2322},
  language     = {eng},
  publisher    = {Nature Publishing Group},
  series       = {Scientific Reports},
  title        = {Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome.},
  url          = {http://dx.doi.org/10.1038/srep11196},
  volume       = {5},
  year         = {2015},
}