Dietary thylakoids reduce visceral fat mass and increase expression of genes involved in intestinal fatty acid oxidation in high-fat fed rats
(2016) In American Journal of Physiology - Regulatory Integrative and Comparative Physiology 311(3). p.618-627- Abstract
Thylakoids reduce body weight gain and body fat accumulation in rodents. This study investigated whether an enhanced oxidation of dietary fat-derived fatty acids in the intestine contributes to the thylakoid effects. Male Sprague-Dawley rats were fed a high-fat diet with (n = 8) or without thylakoids (n = 8) for 2 wk. Body weight, food intake, and body fat were measured, and intestinal mucosa was collected and analyzed. Quantitative realtime PCR was used to measure gene expression levels of key enzymes involved in fatty acid transport, fatty acid oxidation, and ketogenesis. Another set of thylakoid-treated (n = 10) and control rats (n = 10) went through indirect calorimetry. In the first experiment, thylakoidtreated rats (n = 8)... (More)
Thylakoids reduce body weight gain and body fat accumulation in rodents. This study investigated whether an enhanced oxidation of dietary fat-derived fatty acids in the intestine contributes to the thylakoid effects. Male Sprague-Dawley rats were fed a high-fat diet with (n = 8) or without thylakoids (n = 8) for 2 wk. Body weight, food intake, and body fat were measured, and intestinal mucosa was collected and analyzed. Quantitative realtime PCR was used to measure gene expression levels of key enzymes involved in fatty acid transport, fatty acid oxidation, and ketogenesis. Another set of thylakoid-treated (n = 10) and control rats (n = 10) went through indirect calorimetry. In the first experiment, thylakoidtreated rats (n = 8) accumulated 25% less visceral fat than controls. Furthermore, fatty acid translocase (Fat/Cd36), carnitine palmitoyltransferase 1a (Cpt1a), and mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2 (Hmgcs2) genes were upregulated in the jejunum of the thylakoid-treated group. In the second experiment, thylakoid-treated rats (n = 10) gained 17.5% less weight compared with controls and their respiratory quotient was lower, 0.86 compared with 0.91. Thylakoid-intake resulted in decreased food intake and did not cause steatorrhea. These results suggest that thylakoids stimulated intestinal fatty acid oxidation and ketogenesis, resulting in an increased ability of the intestine to handle dietary fat. The increased fatty acid oxidation and the resulting reduction in food intake may contribute to the reduced fat accumulation in thylakoid-treated animals.
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- author
- Stenblom, Eva Lena LU ; Egecioglu, Emil LU ; Montelius, Caroline LU ; Ramachandran, Deepti ; Bonn, Britta ; Weström, Björn LU ; Mansouri, Abdelhak ; Langhans, Wolfgang and Erlanson-Albertsson, Charlotte LU
- organization
- publishing date
- 2016
- type
- Contribution to journal
- publication status
- published
- subject
- keywords
- Energy expenditure, Fat metabolism, Food intake, Plant extracts, Steatorrhea
- in
- American Journal of Physiology - Regulatory Integrative and Comparative Physiology
- volume
- 311
- issue
- 3
- pages
- 618 - 627
- publisher
- American Physiological Society
- external identifiers
-
- pmid:27488889
- wos:000384771800019
- scopus:84986592885
- ISSN
- 0363-6119
- DOI
- 10.1152/ajpregu.00212.2016
- language
- English
- LU publication?
- yes
- id
- 7511f960-79e4-4e57-865a-b3f7ce2020f4
- date added to LUP
- 2016-10-06 10:56:39
- date last changed
- 2024-05-17 13:36:57
@article{7511f960-79e4-4e57-865a-b3f7ce2020f4,
abstract = {{<p>Thylakoids reduce body weight gain and body fat accumulation in rodents. This study investigated whether an enhanced oxidation of dietary fat-derived fatty acids in the intestine contributes to the thylakoid effects. Male Sprague-Dawley rats were fed a high-fat diet with (n = 8) or without thylakoids (n = 8) for 2 wk. Body weight, food intake, and body fat were measured, and intestinal mucosa was collected and analyzed. Quantitative realtime PCR was used to measure gene expression levels of key enzymes involved in fatty acid transport, fatty acid oxidation, and ketogenesis. Another set of thylakoid-treated (n = 10) and control rats (n = 10) went through indirect calorimetry. In the first experiment, thylakoidtreated rats (n = 8) accumulated 25% less visceral fat than controls. Furthermore, fatty acid translocase (Fat/Cd36), carnitine palmitoyltransferase 1a (Cpt1a), and mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase 2 (Hmgcs2) genes were upregulated in the jejunum of the thylakoid-treated group. In the second experiment, thylakoid-treated rats (n = 10) gained 17.5% less weight compared with controls and their respiratory quotient was lower, 0.86 compared with 0.91. Thylakoid-intake resulted in decreased food intake and did not cause steatorrhea. These results suggest that thylakoids stimulated intestinal fatty acid oxidation and ketogenesis, resulting in an increased ability of the intestine to handle dietary fat. The increased fatty acid oxidation and the resulting reduction in food intake may contribute to the reduced fat accumulation in thylakoid-treated animals.</p>}},
author = {{Stenblom, Eva Lena and Egecioglu, Emil and Montelius, Caroline and Ramachandran, Deepti and Bonn, Britta and Weström, Björn and Mansouri, Abdelhak and Langhans, Wolfgang and Erlanson-Albertsson, Charlotte}},
issn = {{0363-6119}},
keywords = {{Energy expenditure; Fat metabolism; Food intake; Plant extracts; Steatorrhea}},
language = {{eng}},
number = {{3}},
pages = {{618--627}},
publisher = {{American Physiological Society}},
series = {{American Journal of Physiology - Regulatory Integrative and Comparative Physiology}},
title = {{Dietary thylakoids reduce visceral fat mass and increase expression of genes involved in intestinal fatty acid oxidation in high-fat fed rats}},
url = {{http://dx.doi.org/10.1152/ajpregu.00212.2016}},
doi = {{10.1152/ajpregu.00212.2016}},
volume = {{311}},
year = {{2016}},
}