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Safety of the probiotic strain Lactobacillus plantarum DSM 9843 (strain 299v) in an endocarditis animal model

Adawi, Diya LU ; Molin, Göran LU ; Ahrné, Siv LU and Jeppsson, Bengt LU (2002) In Microbial Ecology in Health and Disease 14(1). p.50-53
Abstract
Objective: Lactobacilli are often considered to be beneficial or non-pathogenic to man, with small numbers of human infections being reported, including septicemia and infective endocarditis. To verify the safety of Lactobacillus plantarum DSM 9843 (=strain 299v) administration, we used a well-known endocarditis animal model. Design: Experimental study using Sprague-Dawley rats. Setting: University Hospital, Sweden. Interventions: A catheter was passed down the right common carotid artery into the lumen of the left ventricle. The catheter was tied in place and the neck incision was closed. After 48 h, 0.5 ml of a bacterial suspension was injected through the tail vein. In the endocarditis control we inoculated Staphylococcus lugdunesis... (More)
Objective: Lactobacilli are often considered to be beneficial or non-pathogenic to man, with small numbers of human infections being reported, including septicemia and infective endocarditis. To verify the safety of Lactobacillus plantarum DSM 9843 (=strain 299v) administration, we used a well-known endocarditis animal model. Design: Experimental study using Sprague-Dawley rats. Setting: University Hospital, Sweden. Interventions: A catheter was passed down the right common carotid artery into the lumen of the left ventricle. The catheter was tied in place and the neck incision was closed. After 48 h, 0.5 ml of a bacterial suspension was injected through the tail vein. In the endocarditis control we inoculated Staphylococcus lugdunesis CCUG 25349T (T = type strain) and L. plantarum 299v was injected in the blood in L. plantarum group. Main outcome measures: Rats were sacrificed 96 h later and samples were taken from the heart, blood and catheter for bacterial culture and Randomly Amplified Polymorphic DNA (RAPD) evaluation. Results: L. plantarum 299v was injected in the blood but no lactobacilli were found in the heart, blood or catheter after 96 h. RAPD evaluation showed that S. lugdunesis CCUG 25349T was isolated from both blood and heart. Conclusion: The results showed that L. plantarum 299v has no role in the tested endocarditis animal model, which indicates the safety of L. plantarum 299v. (Less)
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author
; ; and
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Endocarditis, L. Plantarum 299v, Bacteraemia
in
Microbial Ecology in Health and Disease
volume
14
issue
1
pages
50 - 53
publisher
Taylor & Francis
external identifiers
  • scopus:0036230209
ISSN
0891-060X
DOI
10.1080/089106002760002766
language
English
LU publication?
yes
additional info
The information about affiliations in this record was updated in December 2015. The record was previously connected to the following departments: Surgery Research Unit (013242220), Emergency medicine/Medicine/Surgery (013240200), Department of Food Technology (011001210), Food Technology (011001017), Applied Nutrition and Food Chemistry (011001300)
id
3f03c1c3-bbcf-4f3b-ba19-89c57549276a (old id 756388)
date added to LUP
2016-04-01 12:24:51
date last changed
2023-09-02 06:50:59
@article{3f03c1c3-bbcf-4f3b-ba19-89c57549276a,
  abstract     = {{Objective: Lactobacilli are often considered to be beneficial or non-pathogenic to man, with small numbers of human infections being reported, including septicemia and infective endocarditis. To verify the safety of Lactobacillus plantarum DSM 9843 (=strain 299v) administration, we used a well-known endocarditis animal model. Design: Experimental study using Sprague-Dawley rats. Setting: University Hospital, Sweden. Interventions: A catheter was passed down the right common carotid artery into the lumen of the left ventricle. The catheter was tied in place and the neck incision was closed. After 48 h, 0.5 ml of a bacterial suspension was injected through the tail vein. In the endocarditis control we inoculated Staphylococcus lugdunesis CCUG 25349T (T = type strain) and L. plantarum 299v was injected in the blood in L. plantarum group. Main outcome measures: Rats were sacrificed 96 h later and samples were taken from the heart, blood and catheter for bacterial culture and Randomly Amplified Polymorphic DNA (RAPD) evaluation. Results: L. plantarum 299v was injected in the blood but no lactobacilli were found in the heart, blood or catheter after 96 h. RAPD evaluation showed that S. lugdunesis CCUG 25349T was isolated from both blood and heart. Conclusion: The results showed that L. plantarum 299v has no role in the tested endocarditis animal model, which indicates the safety of L. plantarum 299v.}},
  author       = {{Adawi, Diya and Molin, Göran and Ahrné, Siv and Jeppsson, Bengt}},
  issn         = {{0891-060X}},
  keywords     = {{Endocarditis; L. Plantarum 299v; Bacteraemia}},
  language     = {{eng}},
  number       = {{1}},
  pages        = {{50--53}},
  publisher    = {{Taylor & Francis}},
  series       = {{Microbial Ecology in Health and Disease}},
  title        = {{Safety of the probiotic strain Lactobacillus plantarum DSM 9843 (strain 299v) in an endocarditis animal model}},
  url          = {{http://dx.doi.org/10.1080/089106002760002766}},
  doi          = {{10.1080/089106002760002766}},
  volume       = {{14}},
  year         = {{2002}},
}