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Modeling electroporation of the non-treated and vacuum impregnated heterogeneous tissue of spinach leaves

Dymek, Katarzyna LU ; Rems, Lea; Zorec, Barbara; Dejmek, Petr LU ; Gomez, Federico LU and Miklavcic, Damijan (2015) In Innovative Food Science & Emerging Technologies 29. p.55-64
Abstract
Uniform electroporation of the heterogeneous structure of spinach leaf cross section is a technological challenge that is addressed in this investigation. Three dimensional models were created with cells arranged in specific tissue types, considering a leaf with its air fraction and a leaf where the air fraction was replaced by a solution of known properties using vacuum impregnation. The models were validated before electroporation, in the frequency domain, where alternating voltage and current signal at frequencies from 20 Hz to I MHz were used to measure conductivity of the tissue. They were also validated through measurements of current during electroporation when a single 250 mu s rectangular pulse with amplitudes ranging from 50 to... (More)
Uniform electroporation of the heterogeneous structure of spinach leaf cross section is a technological challenge that is addressed in this investigation. Three dimensional models were created with cells arranged in specific tissue types, considering a leaf with its air fraction and a leaf where the air fraction was replaced by a solution of known properties using vacuum impregnation. The models were validated before electroporation, in the frequency domain, where alternating voltage and current signal at frequencies from 20 Hz to I MHz were used to measure conductivity of the tissue. They were also validated through measurements of current during electroporation when a single 250 mu s rectangular pulse with amplitudes ranging from 50 to 500 V was applied. Model validations show that both the frequency dependent conductivity and electroporation are well predicted. The importance of the wax layer and stomata in the model is thoroughly discussed. Industrial relevance: Our aim was to investigate electroporation of the spinach leaf by developing a model which would enable us to meet the technological challenge of achieving uniform electroporation in a highly heterogeneous structure in the context of a process aimed at improving freezing stability of plant foods. Pulsed electric field treatment may be used to introduce the cryoprotectant molecules into the cells, and hence improve the structure and properties of frozen food plants. (C) 2014 Elsevier Ltd. All rights reserved. (Less)
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author
organization
publishing date
type
Contribution to journal
publication status
published
subject
keywords
Spinach leaf, Electroporation, Numerical modeling, Vacuum impregnation
in
Innovative Food Science & Emerging Technologies
volume
29
pages
55 - 64
publisher
Elsevier
external identifiers
  • wos:000356740800008
  • scopus:84929517691
ISSN
1466-8564
DOI
10.1016/j.ifset.2014.08.006
language
English
LU publication?
yes
id
7121e502-ffb3-4b97-8f74-ab67347c4e0d (old id 7596452)
date added to LUP
2015-07-23 08:54:49
date last changed
2017-10-01 04:14:26
@article{7121e502-ffb3-4b97-8f74-ab67347c4e0d,
  abstract     = {Uniform electroporation of the heterogeneous structure of spinach leaf cross section is a technological challenge that is addressed in this investigation. Three dimensional models were created with cells arranged in specific tissue types, considering a leaf with its air fraction and a leaf where the air fraction was replaced by a solution of known properties using vacuum impregnation. The models were validated before electroporation, in the frequency domain, where alternating voltage and current signal at frequencies from 20 Hz to I MHz were used to measure conductivity of the tissue. They were also validated through measurements of current during electroporation when a single 250 mu s rectangular pulse with amplitudes ranging from 50 to 500 V was applied. Model validations show that both the frequency dependent conductivity and electroporation are well predicted. The importance of the wax layer and stomata in the model is thoroughly discussed. Industrial relevance: Our aim was to investigate electroporation of the spinach leaf by developing a model which would enable us to meet the technological challenge of achieving uniform electroporation in a highly heterogeneous structure in the context of a process aimed at improving freezing stability of plant foods. Pulsed electric field treatment may be used to introduce the cryoprotectant molecules into the cells, and hence improve the structure and properties of frozen food plants. (C) 2014 Elsevier Ltd. All rights reserved.},
  author       = {Dymek, Katarzyna and Rems, Lea and Zorec, Barbara and Dejmek, Petr and Gomez, Federico and Miklavcic, Damijan},
  issn         = {1466-8564},
  keyword      = {Spinach leaf,Electroporation,Numerical modeling,Vacuum impregnation},
  language     = {eng},
  pages        = {55--64},
  publisher    = {Elsevier},
  series       = {Innovative Food Science & Emerging Technologies},
  title        = {Modeling electroporation of the non-treated and vacuum impregnated heterogeneous tissue of spinach leaves},
  url          = {http://dx.doi.org/10.1016/j.ifset.2014.08.006},
  volume       = {29},
  year         = {2015},
}